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Accueil du site > Animations Scientifiques > Séminaires 2015 > Xiaobo Wang - Controlling mechanism of basal actomyosin oscillation during Drosophila ovary development

Xiaobo Wang - Controlling mechanism of basal actomyosin oscillation during Drosophila ovary development

Speaker : Xiaobo Wang, LBCMCP Université de Toulouse

When : Thursday 28th May at 11 am

Where : Salle CO23 (grande salle de réunions du CBP rez-de-chaussée LR6)

Title : Controlling mechanism of basal actomyosin oscillation during Drosophila ovary development

In epithelia, individual cell is split into apical and basolateral regions. Recently, periodic contraction of actomyosin network has been described to occur in apical domain of different epithelial cells in order to achieve various morphological processes, such as apical constriction and germ band intercalation. Previously we demonstrated a pulsatile actomyosin contraction in epithelial basal surface to induce the underlying tissue elongation during Drosophila ovary development. However, the molecular mechanism controlling this basal oscillation remains unclear. Here, we firstly explored the controlling mechanism of basal actomyosin oscillation. We found that Integrin/Talin complex, but not E-cadherin,function to control the upstream Rho1 activity at plasma membrane. Within the Rho1 downstream signaling pathway, ROCK was the functional link between plasma membrane Rho1 and cortex Myo-II. During a periodic contraction cycle, ROCK shuttled between plasma membrane and cytosol, which was followed byactomyosin contraction flow. This indicates that shuttle movements of ROCK might control their activation and inhibition status. In addition, we recently started to explore the signals that might control the distribution patterns of myosin-oscillating epithelial cells, and our preliminary data indicate that both Dpp signaling pathway and Toll2 are critical in this control. Finally, we found that an interesting intercellular filopodia structure, controlled by Cdc42 activity, is distributed between myosin-oscillating epithelial cells ; and this structure seems to be important in control of coordinated actomyosin oscillation between cells.