Laboratoire JOLIOT-CURIE https://www.ens-lyon.fr/Joliot-Curie/ fr SPIP - www.spip.net Jose Paulo Farinha - Very bright, very stable : very useful http://www.ens-lyon.fr/Joliot-Curie/spip.php?article299 http://www.ens-lyon.fr/Joliot-Curie/spip.php?article299 2015-08-24T15:37:07Z text/html fr Séminaires 2015 Speaker : Jose Paulo FARINHA - Centro de Quimica-Fisica Molecular & Institute of Nanosciences and Nanotechnology, IST, University of Lisbon, PORTUGAL <br />When : Thursday 27th august at 11 am <br />Where : Salle CO23 (grande salle de réunions du CBP rez-de-chaussée LR6) <br />Title : Very bright, very stable : very useful <br />Luminescent nanomaterials are used in many applications, from advanced imaging techniques to biodiagnostic, etc. The ability to increase the brightness and photostability of these materials (...) - <a href="http://www.ens-lyon.fr/Joliot-Curie/spip.php?rubrique75" rel="directory">Séminaires 2015</a> <div class='rss_texte'><p class="spip">Speaker : Jose Paulo FARINHA - Centro de Quimica-Fisica Molecular & Institute of Nanosciences and Nanotechnology, IST, University of Lisbon, PORTUGAL</p> <p class="spip">When : Thursday 27th august at 11 am</p> <p class="spip">Where : Salle CO23 (grande salle de réunions du CBP rez-de-chaussée LR6)</p> <p class="spip">Title : Very bright, very stable : very useful</p> <p class="spip">Luminescent nanomaterials are used in many applications, from advanced imaging techniques to biodiagnostic, etc. The ability to increase the brightness and photostability of these materials have direct impact on their performance, lowering the limit of detection in diagnostic applications and allowing their use in demanding laser scanning imaging techniques. This communication will focus on the use of different approaches to obtain highly fluorescent materials, based on polymers, silica nanoparticles and hybrid nanostructures.</p></div> Xiaobo Wang - Controlling mechanism of basal actomyosin oscillation during Drosophila ovary development http://www.ens-lyon.fr/Joliot-Curie/spip.php?article298 http://www.ens-lyon.fr/Joliot-Curie/spip.php?article298 2015-05-07T13:31:39Z text/html fr Séminaires 2015 Speaker : Xiaobo Wang, LBCMCP Université de Toulouse <br />When : Thursday 28th May at 11 am <br />Where : Salle CO23 (grande salle de réunions du CBP rez-de-chaussée LR6) <br />Title : Controlling mechanism of basal actomyosin oscillation during Drosophila ovary development <br />In epithelia, individual cell is split into apical and basolateral regions. Recently, periodic contraction of actomyosin network has been described to occur in apical domain of different epithelial cells in order to achieve various (...) - <a href="http://www.ens-lyon.fr/Joliot-Curie/spip.php?rubrique75" rel="directory">Séminaires 2015</a> <div class='rss_texte'><p class="spip">Speaker : Xiaobo Wang, LBCMCP Université de Toulouse</p> <p class="spip">When : Thursday 28th May at 11 am</p> <p class="spip">Where : Salle CO23 (grande salle de réunions du CBP rez-de-chaussée LR6)</p> <p class="spip">Title : Controlling mechanism of basal actomyosin oscillation during Drosophila ovary development</p> <p class="spip">In epithelia, individual cell is split into apical and basolateral regions. Recently, periodic contraction of actomyosin network has been described to occur in apical domain of different epithelial cells in order to achieve various morphological processes, such as apical constriction and germ band intercalation. Previously we demonstrated a pulsatile actomyosin contraction in epithelial basal surface to induce the underlying tissue elongation during Drosophila ovary development. However, the molecular mechanism controlling this basal oscillation remains unclear. Here, we firstly explored the controlling mechanism of basal actomyosin oscillation. We found that Integrin/Talin complex, but not E-cadherin,function to control the upstream Rho1 activity at plasma membrane. Within the Rho1 downstream signaling pathway, ROCK was the functional link between plasma membrane Rho1 and cortex Myo-II. During a periodic contraction cycle, ROCK shuttled between plasma membrane and cytosol, which was followed byactomyosin contraction flow. This indicates that shuttle movements of ROCK might control their activation and inhibition status. In addition, we recently started to explore the signals that might control the distribution patterns of myosin-oscillating epithelial cells, and our preliminary data indicate that both Dpp signaling pathway and Toll2 are critical in this control. Finally, we found that an interesting intercellular filopodia structure, controlled by Cdc42 activity, is distributed between myosin-oscillating epithelial cells ; and this structure seems to be important in control of coordinated actomyosin oscillation between cells.</p></div> Laurent Heux - Bioinspired materials obtained by self-assembling properties of elementary bricks : from the primary wall to the iridescence of beetle shells. http://www.ens-lyon.fr/Joliot-Curie/spip.php?article297 http://www.ens-lyon.fr/Joliot-Curie/spip.php?article297 2015-01-12T15:45:32Z text/html fr Séminaires 2015 Speaker : Laurent Heux, CERMAV-CNRS, Grenoble heux(at)cermav.cnrs.fr <br />When : Thursday 22nd January at 11 am <br />Where : Salle CO23 (grande salle de réunions du CBP rez-de-chaussée LR6) <br />Title : Bioinspired materials obtained by self-assembling properties of elementary bricks : from the primary wall to the iridescence of beetle shells. <br />Natural structures exhibit spectacular properties like, for instance, the iridescence of beetle shells, and can therefore be a source of inspiration to design (...) - <a href="http://www.ens-lyon.fr/Joliot-Curie/spip.php?rubrique75" rel="directory">Séminaires 2015</a> <div class='rss_texte'><p class="spip">Speaker : Laurent Heux, CERMAV-CNRS, Grenoble heux(at)cermav.cnrs.fr</p> <p class="spip">When : Thursday 22nd January at 11 am</p> <p class="spip">Where : Salle CO23 (grande salle de réunions du CBP rez-de-chaussée LR6)</p> <p class="spip">Title : Bioinspired materials obtained by self-assembling properties of elementary bricks : from the primary wall to the iridescence of beetle shells.</p> <p class="spip">Natural structures exhibit spectacular properties like, for instance, the iridescence of beetle shells, and can therefore be a source of inspiration to design innovative materials. In several situations, those structures are obtained by smart assemblies of crystalline polysaccharides in association with other polymers. In vivo, those systems are designed to ensure support and protection of a great number of organisms like in plant cells and exoskeletons of insects or crustaceans, and are probably the most widespread architectures in the living world. Starting from elementary bricks, it's possible to reproduce in vitro at least some of the natural structures. For instance, we have elaborated multilayered assemblies mimicking the primary wall , taking advantage of the specific interaction between cellulose nanocrystals and xyloglucan, a common hemicelluloses present in dicotyledons. By deposition of this 2D films on templates like emulsion droplets or vesicles, artificial plant cells, at least simplified models with spherical shape, can be obtained. A great number of parameters can be then modified in a controlled manner, like the nature of the hemicelluloses, their potential covalent binding to cellulose and the deposition method in presence of external constraints. In a totally different domain, the self-organization of cellulose nanocrystals in chiral nematic structures can be used to reproduce the helical structures that can be found in certain organisms. By tuning the interaction between the particles and using external fields, it is possible to mimick the iridescent properties exhibited by the beetle shells or some plants like the Pollia fruit.</p></div> Nouvel article http://www.ens-lyon.fr/Joliot-Curie/spip.php?article296 http://www.ens-lyon.fr/Joliot-Curie/spip.php?article296 2015-01-08T12:42:26Z text/html fr Séminaires 2015 - <a href="http://www.ens-lyon.fr/Joliot-Curie/spip.php?rubrique75" rel="directory">Séminaires 2015</a> Magali Suzanne - Développement de la patte de la drosophile. Implication de la mort cellulaire. Modelisation du processus. http://www.ens-lyon.fr/Joliot-Curie/spip.php?article295 http://www.ens-lyon.fr/Joliot-Curie/spip.php?article295 2014-10-23T16:33:08Z text/html fr Séminaires 2014 Speaker : Magali Suzanne - LBCMCP - Toulouse <br />When : Thursday 13th november at 11 am <br />Where : Salle CO23 (grande salle de réunions du CBP rez-de-chaussée LR6) <br />Title : Développement de la patte de la drosophile. Implication de la mort cellulaire. Modelisation du processus. <br />Epithelium folding is a basic morphogenetic event essential to transform simple 2D epithelial sheets into 3D structures, in both vertebrates and invertebrates1,2. Folding has been shown to rely either on apical junction basal (...) - <a href="http://www.ens-lyon.fr/Joliot-Curie/spip.php?rubrique70" rel="directory">Séminaires 2014</a> <div class='rss_texte'><p class="spip">Speaker : Magali Suzanne - LBCMCP - Toulouse</p> <p class="spip">When : Thursday 13th november at 11 am</p> <p class="spip">Where : Salle CO23 (grande salle de réunions du CBP rez-de-chaussée LR6)</p> <p class="spip">Title : Développement de la patte de la drosophile. Implication de la mort cellulaire. Modelisation du processus.</p> <p class="spip">Epithelium folding is a basic morphogenetic event essential to transform simple 2D epithelial sheets into 3D structures, in both vertebrates and invertebrates1,2. Folding has been shown to rely either on apical junction basal shifting or apical constriction that can be associated with baso-lateral shortening 3-6. The resulting cell shape changes depend on Myosin II redistribution which could be driven by mechanical signals7. Yet, the initial cellular mechanisms that trigger and coordinate cell remodelling remain largely unknown.</p> <p class="spip">Here, we unravel the active role of apoptotic cells in initiating morphogenesis, thus revealing a novel mechanism of epithelium folding. We show that, in a live developing tissue, apoptotic cells exert a transient pulling force upon the apical surface of the epithelium through an unexpected maintenance of their adherens junctions which link up with an highly dynamic apico-basal Myosin II cable, specific to apoptotic cells. The apoptotic cells then induce a non-autonomous increase in tissue tension and apical constriction in the surrounding tissue, eventually resulting in epithelium folding. By integrating data from a theoretical biophysical 3D model and in vivo ectopic apoptosis experiments, our data further reveal the importance of the cumulative effect of apoptotic cells in converting a naive, flat epithelium into a folded one.</p> <p class="spip">Together, our results identify an apoptotic Myosin II dependent signal as the initial signal responsible for cortical Myosin II apical stabilisation, leading to cell reorganisation and tissue folding. This work further reveals that, far from being passively eliminated as generally assumed (e.g. during digit individualisation8), apoptotic cells actively influence their surroundings and trigger tissue remodelling through regulation of tissue tension.</p></div> Grégoire Malandain - Towards population studies in embryogenesis : a cell-to-cell mapping method for ascidian embryos http://www.ens-lyon.fr/Joliot-Curie/spip.php?article294 http://www.ens-lyon.fr/Joliot-Curie/spip.php?article294 2014-10-23T16:29:30Z text/html fr Séminaires 2014 Speaker : Grégoire Malandain - Morpheme team Laboratoire I3S - INRIA <br />When : Thursday 6th november at 11 am <br />Where : Salle CO23 (grande salle de réunions du CBP rez-de-chaussée LR6) <br />Title : Towards population studies in embryogenesis : a cell-to-cell mapping method for ascidian embryos <br />Nowadays, microscopy techniques allow to image temporal 3D stacks of developing organs or embryos at the cellular level and with an acquisition frequency that enables to retrieve the cell lineages. Imaging a (...) - <a href="http://www.ens-lyon.fr/Joliot-Curie/spip.php?rubrique70" rel="directory">Séminaires 2014</a> <div class='rss_texte'><p class="spip">Speaker : Grégoire Malandain - Morpheme team Laboratoire I3S - INRIA</p> <p class="spip">When : Thursday 6th november at 11 am</p> <p class="spip">Where : Salle CO23 (grande salle de réunions du CBP rez-de-chaussée LR6)</p> <p class="spip">Title : Towards population studies in embryogenesis : a cell-to-cell mapping method for ascidian embryos</p> <p class="spip">Nowadays, microscopy techniques allow to image temporal 3D stacks of developing organs or embryos at the cellular level and with an acquisition frequency that enables to retrieve the cell lineages. Imaging a number of organs or embryos with several conditions may help to decipher the various components that drive the development, however this requires to distinguish between the inter-individual variability and the components' effects, enforcing the need for the comparison between individuals.</p> <p class="spip">We will present a method that allows first to segment the cells from microscopy images, second to extract the symmetry plane of embryos at early stages, and third to retrieve a cell-to-cell mapping between two embryos.</p></div> Sebastian Schornack - How to be a good host – microbial and host strategies to control colonisation http://www.ens-lyon.fr/Joliot-Curie/spip.php?article293 http://www.ens-lyon.fr/Joliot-Curie/spip.php?article293 2014-10-23T16:26:26Z text/html fr Séminaires 2014 Speaker :Sebastian Schornack - Sainsbury lab, Cambridge UK (http://www.schornacklab.net) <br />When : Thursday 4th december at 11 am <br />Where : Salle CO23 (grande salle de réunions du CBP rez-de-chaussée LR6) <br />Title : How to be a good host – microbial and host strategies to control colonisation <br />Animals and plants are constantly exposed to a plethora of microbes. Pathogens successfully break the hosts defence mechanisms, enter and colonise the tissues causing diseases. However, some microorganisms are (...) - <a href="http://www.ens-lyon.fr/Joliot-Curie/spip.php?rubrique70" rel="directory">Séminaires 2014</a> <div class='rss_texte'><p class="spip">Speaker :Sebastian Schornack - Sainsbury lab, Cambridge UK (http://www.schornacklab.net)</p> <p class="spip">When : Thursday 4th december at 11 am</p> <p class="spip">Where : Salle CO23 (grande salle de réunions du CBP rez-de-chaussée LR6)</p> <p class="spip">Title : How to be a good host – microbial and host strategies to control colonisation</p> <p class="spip">Animals and plants are constantly exposed to a plethora of microbes. Pathogens successfully break the hosts defence mechanisms, enter and colonise the tissues causing diseases. However, some microorganisms are hosted for beneficial purposes.Almost all land plants team up with fungi to establish long lasting beneficial symbiosis in roots termed mycorrhiza. A hallmark of successful pathogens of animals and plants are effector proteins.These microbial proteins are transferred into the host cell, where they “convince” the host to permit microbial colonisation. Studying processes targeted by microbial effectors allows us to reveal these strategies.Good examples are bacterial TAL effectors (http://www.scoop.it/tal-effector-science), which travel into the host cell nucleus to induce host susceptibility genes. My group's current research focusses on understanding plant processes which support entry and colonisation by fungi and other filamentous microbes. Their modulationwill enable us to support beneficial fungi and to protect our crops from diseases.We study interactions of mycorrhiza fungi as well as pathogenic oomycetes with legumes, cereals and tobacco. In this talk I would like to introduce our research, provide some cool examples how microbes reprogram plants and which plant processes are required for colonisation. I will also touch on the potential of plant-microbe studies to intersect with and generate tools for other plant and animal research.</p></div> soutenance HDR Karine Monier Décembre 2014 http://www.ens-lyon.fr/Joliot-Curie/spip.php?article292 http://www.ens-lyon.fr/Joliot-Curie/spip.php?article292 2014-10-01T13:08:10Z text/html fr Habilitations à Diriger les Recherches - <a href="http://www.ens-lyon.fr/Joliot-Curie/spip.php?rubrique30" rel="directory">Habilitations à Diriger les Recherches</a> Anne-Françoise Mingotaud - Polymeric self-assemblies for photodynamic therapy http://www.ens-lyon.fr/Joliot-Curie/spip.php?article291 http://www.ens-lyon.fr/Joliot-Curie/spip.php?article291 2014-10-01T13:06:42Z text/html fr Séminaires 2014 Speaker : Anne-Françoise Mingotaud, Laboratoire des IMRCP, Université Paul Sabatier, Toulouse <br />When : Thursday 23rd october at 11 am <br />Where : Salle CO23 (grande salle de réunions du CBP rez-de-chaussée LR6) <br />Title : Polymeric self-assemblies for photodynamic therapy <br />Thanks to the easy access to wide families of synthetic biocompatible polymers, polymeric self-assemblies are increasingly assessed in biomedical applications, such as drug delivery or imaging. The presentation will enlighten our (...) - <a href="http://www.ens-lyon.fr/Joliot-Curie/spip.php?rubrique70" rel="directory">Séminaires 2014</a> <div class='rss_texte'><p class="spip">Speaker : Anne-Françoise Mingotaud, Laboratoire des IMRCP, Université Paul Sabatier, Toulouse</p> <p class="spip">When : Thursday 23rd october at 11 am</p> <p class="spip">Where : Salle CO23 (grande salle de réunions du CBP rez-de-chaussée LR6)</p> <p class="spip">Title : Polymeric self-assemblies for photodynamic therapy</p> <p class="spip">Thanks to the easy access to wide families of synthetic biocompatible polymers, polymeric self-assemblies are increasingly assessed in biomedical applications, such as drug delivery or imaging. The presentation will enlighten our expertise in such self-assemblies and their use for photodynamic therapy. Polymers such as poly(ethyleneoxide-b--caprolactone), poly(ethyleneoxide-b-lactide), poly(ethyleneoxide-b-styrene) or poly(ethyleneoxide-b-methylmethacrylate) are routinely used in our team. The presentation will enlighten the different characterizations we perform, going from classical ones such as light scattering or electron microscopy, to more unsusual ones like asymmettrical flow field flow fractionation. Exemples of biological tests will also be presented, such as photodynamic therapy efficiency on either 2D or 3D cell culture.</p></div> Nouvel article http://www.ens-lyon.fr/Joliot-Curie/spip.php?article290 http://www.ens-lyon.fr/Joliot-Curie/spip.php?article290 2014-05-21T16:38:32Z text/html fr Biophysics and development. Project Leader : Arezki Boudaoud. - <a href="http://www.ens-lyon.fr/Joliot-Curie/spip.php?rubrique53" rel="directory">Biophysics and development. Project Leader : Arezki Boudaoud.</a>