Annuaire
Pascale LESAGE - Hopital Saint Louis - Transposition and spatial genome organization
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Le 06/11/2020, de 11:00 à 12:00 |
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| S'adresser à | Salle des Thèses |
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Transposable elements (TEs) are present in most genomes and have the ability to move from one chromosome location to another through independent replication of their sequences. Although new integrations may be detrimental to genetic integrity and cell fate, TEs have also catalyzed the evolution of genome and chromatin structure, gene-regulatory networks and gene functions. Deciphering whether TEs have a target-site preference and how this is achieved is critical to understand their implication in genome plasticity and host–parasite interactions but also for TE-based genome engineering applications.
Retrotransposons are the main active TEs in mammals and yeasts, and are structurally and functionally related to retroviruses. The Ty1 LTR-retrotransposon of S. cerevisiae preferentially integrates into a 1-kb window upstream of RNA polymerase III-transcribed genes, which are essentially the genes encoding tRNAs. Because these genes are in multicopy and thus are individually non-essential, this integration pattern allows Ty1 to replicate and yet minimizes genetic damage to its host. Our current work aims at characterizing the multiple factors that cooperate in guiding Ty1 to its preferred sites of integration.
Address : Team Genome Biology : from mobile DNA to chromosome dynamics (GeBi), INSERM U944, CNRS UMR 7212, Genomes & Cell Biology of Disease Unit, Institut de Recherche Saint-Louis, Université de Paris, Hôpital Saint-Louis, Paris, France.
https://gencelldis.fr
pascale.lesage@inserm.fr
Hôte : A. Piazza