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Pablo NAVARRO, Developmental and Stem Cell Biology, EPIC unit, Institut Pasteur, Paris, France
| When |
Jun 21, 2019
from 11:00 to 12:00 |
|---|---|
| Contact Name | Equipe Bernard - Amphi Schrodinger |
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Replication and mitosis ensure the equal partition of the genetic information between daughter cells. However, both events entail major chromatin reorganisation, the loss of TF binding, and the invasion of regulatory elements by nucleosomes. While the importance of self-propagated epigenetic marks in guiding the re-establishment of gene regulatory complexes after replication and mitosis is commonly accepted, whether and how sequence-specific Transcription Factors (TFs) are directly involved is unclear. We will show that the TFs Esrrb and CTCF, but not others like Oct4 and Sox2, display an extremely efficient capacity to displace nucleosomes from their binding sites and locally organise large and phased nucleosomal arrays in interphase, minutes after the passage of the replication fork, and also during mitosis. These results unequivocally link TF activity to the preservation of nucleosome organisation throughout the cell-cycle, complementing epigenetic mechanisms that propagate chromatin modifications associated with transcriptional control.
Contact : Pascal Bernard