Annuaire
TCTP and CSN4 control cell cycle progression and development by regulating CULLIN1 neddylation in plants and animals.
PLoS Genet, 15(1):e1007899.
Translationally Controlled Tumor Protein (TCTP) controls growth by regulating theG1/S transition during cell cycle progression. Our genetic interaction studies showthat TCTP fulfills this role by interacting with CSN4, a subunit of the COP9Signalosome complex, known to influence CULLIN-RING ubiquitin ligases activity bycontrolling CULLIN (CUL) neddylation status. In agreement with these data,downregulation of CSN4 in Arabidopsis and in tobacco cells leads to delayed G1/Stransition comparable to that observed when TCTP is downregulated. Loss-of-functionof AtTCTP leads to increased fraction of deneddylated CUL1, suggesting that AtTCTPinterferes negatively with COP9 function. Similar defects in cell proliferation andCUL1 neddylation status were observed in Drosophila knockdown for dCSN4 or dTCTP,respectively, demonstrating a conserved mechanism between plants and animals.Together, our data show that CSN4 is the missing factor linking TCTP to the controlof cell cycle progression and cell proliferation during organ development and openperspectives towards understanding TCTP's role in organ development and disordersassociated with TCTP miss-expression.
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