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RNA polymerase II CTD S2P is dispensable for embryogenesis but mediates exit from developmental diapause in C. elegans.
Sci Adv, 6(50).
Serine 2 phosphorylation (S2P) within the CTD of RNA polymerase II is considered aCdk9/Cdk12-dependent mark required for 3'-end processing. However, the relevance ofCTD S2P in metazoan development is unknown. We show that cdk-12 lesions or afull-length CTD S2A substitution results in an identical phenotype in Caenorhabditiselegans Embryogenesis occurs in the complete absence of S2P, but the hatched larvaearrest development, mimicking the diapause induced when hatching occurs in theabsence of food. Genome-wide analyses indicate that when CTD S2P is inhibited, onlya subset of growth-related genes is not properly expressed. These genes correspondto SL2 trans-spliced mRNAs located in position 2 and over within operons. We showthat CDK-12 is required for maximal occupancy of cleavage stimulatory factornecessary for SL2 trans-splicing. We propose that CTD S2P functions as agene-specific signaling mark ensuring the nutritional control of the C. elegansdevelopmental program.
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