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The fluorescent protein stability assay: an efficient method for monitoring intracellular protein stability.

Armelle Roisin, Samuel Buchsbaum, Vincent Mocquet, and Pierre Jalinot (2021)

Biotechniques, 70(6):336-344.

The stability of intracellular proteins is highly variable, from a few minutes toseveral hours, and can be tightly regulated to respond to external and internalcellular environment changes. Several techniques can be used to study thestability of a specific protein, including pulse-chase labeling and blocking oftranslation. Another approach that has gained interest in recent years is fusinga protein of interest to a fluorescent reporter. In this report, the authorspresent a new version of this approach aimed at optimizing expression andcomparison of the two reporter proteins. The authors show that the system worksefficiently in various cells and can be useful for studying changes in proteinstability and assessing the effects of drugs.

 
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