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You are here: Home / Teams / Posttranscriptional Regulation in Infection and Oncogenesis - Jalinot/Mocquet / Thèmes de recherche / Control of histone expression by INT6 and SLBP (Pierre Jalinot- projet 3)

Control of histone expression by INT6 and SLBP (Pierre Jalinot- projet 3)

Strenghthening the role of INT6 in genome stability, we have shown that INT6 is needed for efficient expression of the histones during the S phase of the cell cycle.

Strenghthening the role of translation in genome stability, we have shown that INT6 is needed for efficient expression of the histones during the S phase of the cell cycle. Histone transcripts are characterized by the absence of a poly(A) tail and the presence at their 3’ end of a stem-loop that binds SLBP. This RNA binding protein plays a key role in maturation, nuclear export and cell cycle-specific translation of the histone mRNAs. By interacting with both MIF4GD and SLBP, INT6 helps to the recruitment of eIF3 and consecutively to translation initiation (Neusiedler et al., RNA, 2012).To further study the degradation of SLBP and the role of INT6, we developed recently an assay based on fluorescent proteins in which SLBP is fused to a RFP variant and coexpressed with EGFP taken as control. Measurement of EGFP and RFP fluorescences by FACS provides a simple assay to assess SLBP stability.