Aller au contenu. | Aller à la navigation

Outils personnels

Navigation
Vous êtes ici : Accueil / Équipes / Regulation of Genome Architecture and Dynamics of Splicing (ReGArDS) - D. Auboeuf / C. Bourgeois / Publications / A conserved splicing mechanism of the LMNA gene controls premature aging.

A conserved splicing mechanism of the LMNA gene controls premature aging.

Isabel C Lopez-Mejia, Valentin Vautrot, Marion De Toledo, Isabelle Behm-Ansmant, Cyril F Bourgeois, Claire L Navarro, Fernando G Osorio, Jose MP Freije, James Stevenin, Annachiara De Sandre-Giovannoli, Carlos Lopez-Otin, Nicolas Levy, Christiane Branlant, and Jamal Tazi (2011)

Hum Mol Genet, 20(23):4540-55.

Hutchinson-Gilford progeria syndrome (HGPS) is a rare genetic disorder phenotypically characterized by many features of premature aging. Most cases of HGPS are due to a heterozygous silent mutation (c.1824C>T; p.Gly608Gly) that enhances the use of an internal 5' splice site (5'SS) in exon 11 of the LMNA pre-mRNA and leads to the production of a truncated protein (progerin) with a dominant negative effect. Here we show that HGPS mutation changes the accessibility of the 5'SS of LMNA exon 11 which is sequestered in a conserved RNA structure. Our results also reveal a regulatory role of a subset of serine-arginine (SR)-rich proteins, including serine-arginine rich splicing factor 1 (SRSF1) and SRSF6, on utilization of the 5'SS leading to lamin A or progerin production and a modulation of this regulation in the presence of the c.1824C>T mutation is shown directly on HGPS patient cells. Mutant mice carryingthe equivalent mutation in the LMNA gene (c.1827C>T) also accumulate progerin and phenocopy the main cellular alterations and clinical defects of HGPS patients. RNAi-induced depletion of SRSF1 in the HGPS-like mouse embryonic fibroblasts (MEFs) allowed progerin reduction and dysmorphic nuclei phenotype correction, whereas SRSF6 depletion aggravated the HGPS-like MEF's phenotype. We demonstratethat changes in the splicing ratio between lamin A and progerin are key factors for lifespan since heterozygous mice harboring the mutation lived longer than homozygous littermates but less than the wild-type. Genetic and biochemical datatogether favor the view that physiological progerin production is under tight control of a conserved splicing mechanism to avoid precocious aging.

 
automatic medline import

Actions sur le document