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Vous êtes ici : Accueil / Équipes / Métabolisme des ARNs au cours de la réponse immunitaire et de l’infection - E. Ricci / Publications / System-wide Profiling of RNA-Binding Proteins Uncovers Key Regulators of Virus Infection.

System-wide Profiling of RNA-Binding Proteins Uncovers Key Regulators of Virus Infection.

Manuel Garcia-Moreno, Marko Noerenberg, Shuai Ni, Aino I Jarvelin, Esther Gonzalez-Almela, Caroline E Lenz, Marcel Bach-Pages, Victoria Cox, Rosario Avolio, Thomas Davis, Svenja Hester, Thibault JM Sohier, Bingnan Li, Gregory Heikel, Gracjan Michlewski, Miguel A Sanz, Luis Carrasco, Emiliano P Ricci, Vicent Pelechano, Ilan Davis, Bernd Fischer, Shabaz Mohammed, and Alfredo Castello (2019)

Mol Cell, 74(1):196-211.e11.

The compendium of RNA-binding proteins (RBPs) has been greatly expanded by the development of RNA-interactome capture (RIC). However, it remained unknown if the complement of RBPs changes in response to environmental perturbations and whether these rearrangements are important. To answer these questions, we developed "comparative RIC" and applied it to cells challenged with an RNA virus called sindbis (SINV). Over 200 RBPs display differential interaction with RNA upon SINV infection. These alterations are mainly driven by the loss of cellular mRNAs andthe emergence of viral RNA. RBPs stimulated by the infection redistribute to viral replication factories and regulate the capacity of the virus to infect. For example, ablation of XRN1 causes cells to be refractory to SINV, while GEMIN5 moonlights as a regulator of SINV gene expression. In summary, RNA availability controls RBP localization and function in SINV-infected cells.

 
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