SD+Dropout
(For 1L)
Difco Yeast Nitrogen Base w/o Amino Acids 6,7g
Glucose 20g
Bacto Agar (if plates) 20g
Dropout (aa mix) 2g
Distilled water qsp 1L
Add NaOH 1N to have pH=5,8 (about 1mL)
Autoclave at 0,5bar
S+Dropout
(without sugar) (For 500L)
Difco Yeast Nitrogen Base w/o Amino Acids 3,35g
Bacto Agar (if plates) 10g
Dropout (aa mix) 1g
Distilled water qsp 450mL
Add NaOH 1N to have pH=5,8 (about 500mL)
Autoclave at 0,5bar
YPD
(for 1L)
Bacto Peptone Difco 10g
Bacto Yeast Extract Difco 10g
Glucose 20g
Bacto Agar (if plates) 20g
Distilled water qsp 1L
Autoclave at 0,5bar
YPD+G418
(for 1L)
Same recipe as above.
After autoclavage let cool down until 55°C
Add G418 to have a final concentration of 200mg/L
(1 mL of 200mg/mL G418 in 1L of YPD)
YPD+NAT
(for 1L)
Same recipe as above.
After autoclavage let cool down until 55°C
Add NAT to have a final concentration of 60mg/L
(300uL of 200mg/mL NAT in 1L of YPD)
YP Gal/Raf
(for 1L
Bacto Peptone Difco 10g
Bacto Yeast Extract Difco 10g
Galactose 20g
Raffinose 10g
Bacto Agar (if plates) 20g
Distilled water qsp 1L
Autoclave at 0,5bar
Kac (for Sporulation)
(for 1L)
Potassium acetate 20g
Bacto Agar 20g
Distilled water qsp 1L
Adjust to have pH=7
Autoclave at 0,5bar
5 FoA
(For 500mL)
Difco Yeast Nitrogen Base w/o Amino Acids 3.35g
Glucose 10g
Bacto Agar 10g
Dropout mix+ALL 1g
Distilled water qsp 500mL
Autoclave at 0,5bar.
Dissolve 500mg of 5FoA.
DROPOUT
Add all the amino acids except 1 or more according to the expected mixture .
Adénine (Ad) 1 g
Uracile (U) 2 g
Alanine (A) 2 g
Arginine (R) 2 g
Aspartate (D) 2 g
Asparagine (N) 2 g
Cystéine (C) 2 g
Glutamate (E) 2 g
Glutamine (Q) 2 g
Glycine (G) 2 g
Histidine (H) 2 g
Isoleucine (I) 2 g
Leucine (L) 4 g
Lysine (K) 2 g
Méthionine (M) 2 g
Phénylalanine (F) 2 g
Proline (P) 2 g
Sérine (S) 2 g
Thréonine (T) 2 g
Tryptophane (W) 2 g
Tyrosine (Y) 2 g
Valine (V) 2 g
DRUGS
Kanamycin (G418 Sulfate)
P25-011, PAA
Stock solution : 200mg/mL ; Final concentration : 200mg/L (1mL in 1L)
NAT (Nourseothricin-sulfate or clonNAT)
5-1000, Gisela Werner
Stock solution : 200mg/mL ; Final concentration : 60mg/L (300uL in 1L)
Hygromycin B
843 555 1g (20mL) in PBS, Roche
Stock solution : 50mg/mL ; Final concentration : 200mg/L (4mL in 1L)
Doxycycline
D-9891, Sigma
Stock solution : 5mg/mL ; Final concentration : 10mg/L (2mL in 1L)
Dernière mise à jour : ( 11-03-2010 )