Recruitment

Environnement scientifique : Localisé à l’ENS de Lyon, dans un environnement multidisciplinaire riche, le Laboratoire de Biologie et de Modélisation de la Cellule (LBMC) est un laboratoire de biologie expérimentale dont l’objet d’étude est la cellule, l’unité fondamentale du vivant. Les 15 équipes du LBMC intègrent dans leurs recherches différents aspects de la vie cellulaire comme la prolifération, la différenciation, et l’interaction avec l’environnement cellulaire dans un contexte normal ou pathologique.

Notre équipe de recherche « Mort cellulaire régulée et génétique de la neurodégénérescence » est dirigée par le Professeur Bertrand Mollereau (http://www.ens-lyon.fr/LBMC/equipes/ApoDroso). Nous nous intéressons aux processus cellulaires conduisant à la mort des neurones dans les processus neurodégénératifs en utilisant des modèles cellulaires et drosophiles de la maladie de Parkinson. Nos résultats soulignent l’importance du métabolisme lipidique et de l’accumulation neuronale de gouttelettes lipidiques au cours de la progression de la pathologie. Nous travaillons actuellement sur les mécanismes conduisant à l’accumulation des gouttelettes lipidiques dans les neurones.

Mots clés : Drosophile, maladie de Parkinson, neurones, métabolisme lipidique

Missions Vous réaliserez des expériences de croisements génétiques, des dissections de rétines et de cerveaux de drosophile, des immuno-marquages. Vous imagerez les résultats des expériences à l’aide d’un microscope confocal de type LSM800 au plateau technique d’imagerie de l’ENS de Lyon (Platim). Finalement, vous analyserez et quantifierez vos résultats à l’aide du logiciel Image J. Vous utiliserez également les techniques de western blot et de qPCR pour évaluer l’expression de gènes candidats. Vous participerez par ailleurs à des missions transversales relatives au fonctionnement de l’équipe comme l’entretien des lignées de drosophile, la gestion des stocks ou les commandes de réactifs.

Profil : Vous êtes motivé-e par la recherche fondamentale, rigoureux-se, autonome, respecteux-se de vos collègues de travail. Une expérience d’au moins 6 mois dans un laboratoire de recherche utilisant le modèle génétique de la drosophile est requise. Des connaissances en microscopie seront appréciées.

Pour postuler : adresser avant le 31 Mars 2023 un CV et une lettre de motivation soulignant l’adéquation entre le profil du poste et votre candidature à nathalie.davoust-nataf[at]ens-lyon.fr et à bertrand.mollereau[at]ens-lyon.fr

Publications du laboratoire ou revue recommandée sur le sujet :

Girard V., Jollivet F., Knittelfelder O., Celle M., Arsac JN, Chatelain G., Van den Brink DM, Baron T., Shevchenko A., Kühnlein R., Davoust N* and Mollereau B.* (*co-corresponding author). Neuronal lipid droplets promote a pathological conversion in alpha-synuclein via a feed-forward mechanism. PloS Genet. 2021

Girard V., Goubard V., Querenet M., Seugnet L., Pays L., Nataf S., Dufourd E., Cluet D., Mollereau B. and Davoust N. Split-ends is modulating lipid droplet content in adult Drosophila glial cells and is protective against paraquat toxicity. Sci. Rep. Nov 18;10(1):20023. 2020

Van Den Brink DM, Cubizolle A, Chatelain G, Davoust N, Girard V., Johansen S, Napoletano F, Dourlen P, Guillou L, Angebault-Prouteau C, Bernoud-Hubac N, Guichardant M, Brabet P, Mollereau B. Physiological and pathological roles of FATP-mediated lipid droplets in Drosophila and mice retina. PLoS Genet. 14(9):e1007627. 2018

Islimye E., Girard V., Gould A. P. Functions of stress-induced lipid droplets in the nervous system, Front Cell Dev Biol. Apr 14;10:863907. 2022

Adresse:

Laboratoire de Biologie et de Modélisation de la Cellule
ENS de Lyon
46 allée d’Italie
69007 LYON

Photoreceptors rhabdomers are stained by phalloidin stainings (magenta) and lipid droplets by BODIPY (green).

M2 internship/Funded PhD position in biological physics: Understanding how condensin folds genomes during mitosis

Our group is looking for a PhD candidate in biological physics or computational biology to work on chromosome folding in close collaboration with experimentalists.

Context:

Inside the cellular nucleus, DNA is tightly packed into a polymer-like structure called chromatin. Characterizing how chromatin self-organizes is one of the major challenges faced in recent years by biology. During the last decade, thanks to the development of advanced experimental techniques, major progresses have been realized in our understanding of the multi-scale chromosome organization during interphase. An increasing number of experimental evidences has suggested that the spatio-temporal organization of the genome may play a decisive role in the regulation of gene expression and in diseases. It is therefore of high importance to better characterize the mechanisms driving such organization. In particular, a conserved principle is the folding of the genome into loops by active translocating motors such as condensin and cohesin. However, the nature and the consequences of the interplay between such DNA-binding motors and chromatin remain largely unknown. In this project, we aim to address that question by focusing on the condensin complex during mitosis using an interdisciplinary approach.

Objectives:

The student will develop a research activity on the modeling of chromosome folding and dynamics in eukaryotes to better characterize the role of histones and other chromatin-binding proteins in regulating the condensin loop extrusion activity. It will involve the development of original models coupling statistical and polymer physics, of efficient simulation schemes, and of statistical tools to analyze experimental data. The project will be realized in close collaboration with the group of Pascal Bernard for experimental biology (LBMC, ENS de Lyon) and Olivier Cuvier (CBI, Toulouse) for data analysis.  

Environment:

The candidate will integrate our group ‘Physical Biology of Chromatin’ that mainly focuses on understanding the fundamental bases of chromatin and gene regulation using physical modeling and computational approaches.  Our innovative research is conducted in close interaction with top-leader experimental partners. The group is integrated within the Laboratory of Biology and Modeling of the Cell that aims to characterize the molecular bases underlying the organization and functioning of cellular processes in normal and pathological conditions . It is based at Ecole Normale Supérieure de Lyon, a French top-leading research and educational institute.

Profile of the candidate:

We are looking for a creative and highly motivated candidate with a background in statistical or polymer physics, in computer science or in computational biology. Advanced skills in programming is required and a previous interdisciplinary experience in connection with biological issues would be a plus.

To apply, please send your CV and a motivation letter to Daniel Jost at daniel.jost[at]ens-lyon.fr

Physical Biology of Chromatin’ group
Laboratory of the Biology and Modeling of the Cell
CNRS, ENS de Lyon, France
http://www.ens-lyon.fr/LBMC/equipes/biologie-physique-de-la-chromatine

Postdoc position in biological physics:  Polymer modeling of chromosome organization

Our group is looking for a postdoc in biological physics to work on chromosome folding in close collaboration with experimentalists.

Context:

Inside the cellular nucleus, DNA is tightly packed into a polymer-like structure called chromatin. Characterizing how chromatin self-organizes is one of the major challenges faced in recent years by biology. During the last decade, thanks to the development of advanced experimental techniques, major progresses have been realized in our understanding of the multi-scale chromosome organization during interphase. An increasing number of experimental evidences has suggested that the spatio-temporal organization of the genome may play a decisive role in the regulation of gene expression and in diseases. It is therefore of high importance to better characterize the mechanisms driving such organization.

Objectives:

The postdoctoral fellow will develop a research activity on the modeling of chromosome folding and dynamics in somatic and germ cells. It will involve the development of original models coupling statistical and polymer physics, of efficient simulation schemes, and of statistical tools to analyze experimental data. The candidate will have the opportunity to collaborate with experimental biology groups, working on various species and systems.  

Environment:

The candidate will integrate our group ‘Physical Biology of Chromatin’ that mainly focuses on understanding the fundamental bases of chromatin and gene regulation using physical modeling and computational approaches within a strong interdisciplinary environment.  Our innovative research is conducted in close interaction with top-leader experimental partners. The group is integrated within the Laboratory of Biology and Modeling of the Cell that aims to characterize the molecular bases underlying the organization and functioning of cellular processes in normal and pathological conditions . It is based at Ecole Normale Supérieure de Lyon, a French top-leading research and educational institute.

Profile of the candidate:

We are looking for a creative and highly motivated candidate with a strong background in statistical or polymer physics, in computer science or in computational biology. Advanced skills in programming is required and a previous interdisciplinary experience in connection with biological issues would be a plus.

To apply, please send your CV and a motivation letter to Daniel Jost at daniel.jost[at]ens-lyon.fr

Physical Biology of Chromatin’ group
Laboratory of the Biology and Modeling of the Cell
CNRS, ENS de Lyon, France
http://www.ens-lyon.fr/LBMC/equipes/biologie-physique-de-la-chromatine