LOCUS       1xTUBBYp3'              3434 bp    DNA     circular     13-OCT-2010
DEFINITION  attL3-attR2.
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     VNTDATE|567965161|
COMMENT     VNTDBDATE|567965161|
COMMENT     LSOWNER|
COMMENT     VNTNAME|1xTUBBYp3'|
COMMENT     VNTAUTHORNAME|Demo User|
FEATURES             Location/Qualifiers
     misc_feature    complement(3427..88)
                     /vntifkey="21"
                     /label=attL3
     misc_feature    complement(2508..2631)
                     /vntifkey="21"
                     /label=attR2
     terminator      2152..2179
                     /invitrogen="2590000"
                     /vntifkey="43"
                     /label=rrnB\T2\transcription\terminator
     terminator      2311..2354
                     /invitrogen="2580000"
                     /vntifkey="43"
                     /label=rrnB\T1\transcription\terminator
     promoter        complement(128..144)
                     /invitrogen="2370000"
                     /vntifkey="30"
                     /label=T7\promoter
     primer          complement(125..144)
                     /invitrogen="2100000"
                     /vntifkey="27"
                     /label=T7\primer
     primer          complement(149..165)
                     /invitrogen="2010000"
                     /vntifkey="27"
                     /label=M13\reverse\primer
     primer          2401..2417
                     /invitrogen="2000000"
                     /vntifkey="27"
                     /label=M13\(-40)\forward\primer
     primer          2421..2436
                     /invitrogen="1980000"
                     /vntifkey="27"
                     /label=M13\(-20)\forward\primer
     CDS             278..1087
                     /invitrogen="1210000"
                     /vntifkey="4"
                     /label=Kan(R)
     misc_feature    2634..3422
                     /vntifkey="21"
                     /label=Tubby\domain
BASE COUNT      873 a       827 c       854 g       880 t 
ORIGIN
        1 ttatacaaag ttggcattat aaaaaagcat tgcttatcaa tttgttgcaa cgaacaggtc 
       61 actatcagtc aaaataaaat cattatttgg agctccatgg tagcgttaac gcggccgcga 
      121 tatcccctat agtgagtcgt attacatggt catagctgtt tcctggcagc tctggcccgt 
      181 gtctcaaaat ctctgatgtt acattgcaca agataaaaat atatcatcat gaacaataaa 
      241 actgtctgct tacataaaca gtaatacaag gggtgttatg agccatattc aacgggaaac 
      301 gtcgaggccg cgattaaatt ccaacatgga tgctgattta tatgggtata aatgggctcg 
      361 cgataatgtc gggcaatcag gtgcgacaat ctatcgcttg tatgggaagc ccgatgcgcc 
      421 agagttgttt ctgaaacatg gcaaaggtag cgttgccaat gatgttacag atgagatggt 
      481 cagactaaac tggctgacgg aatttatgcc tcttccgacc atcaagcatt ttatccgtac 
      541 tcctgatgat gcatggttac tcaccactgc gatccccgga aaaacagcat tccaggtatt 
      601 agaagaatat cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg 
      661 gttgcattcg attcctgttt gtaattgtcc ttttaacagc gatcgcgtat ttcgtctcgc 
      721 tcaggcgcaa tcacgaatga ataacggttt ggttgatgcg agtgattttg atgacgagcg 
      781 taatggctgg cctgttgaac aagtctggaa agaaatgcat aaacttttgc cattctcacc 
      841 ggattcagtc gtcactcatg gtgatttctc acttgataac cttatttttg acgaggggaa 
      901 attaataggt tgtattgatg ttggacgagt cggaatcgca gaccgatacc aggatcttgc 
      961 catcctatgg aactgcctcg gtgagttttc tccttcatta cagaaacggc tttttcaaaa 
     1021 atatggtatt gataatcctg atatgaataa attgcagttt catttgatgc tcgatgagtt 
     1081 tttctaatca gaattggtta attggttgta acactggcag agcattacgc tgacttgacg 
     1141 ggacggcgca agctcatgac caaaatccct taacgtgagt tacgcgtcgt tccactgagc 
     1201 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat 
     1261 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga 
     1321 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt 
     1381 ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata 
     1441 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac 
     1501 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg 
     1561 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg 
     1621 tgagcattga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag 
     1681 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct 
     1741 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc 
     1801 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt 
     1861 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg 
     1921 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga 
     1981 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg 
     2041 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg 
     2101 caacgcaatt aatacgcgta ccgctagcca ggaagagttt gtagaaacgc aaaaaggcca 
     2161 tccgtcagga tggccttctg cttagtttga tgcctggcag tttatggcgg gcgtcctgcc 
     2221 cgccaccctc cgggccgttg cttcacaacg ttcaaatccg ctcccggcgg atttgtccta 
     2281 ctcaggagag cgttcaccga caaacaacag ataaaacgaa aggcccagtc ttccgactga 
     2341 gcctttcgtt ttatttgatg cctggcagtt ccctactctc gcgttaacgc tagcatggat 
     2401 gttttcccag tcacgacgtt gtaaaacgac ggccagtctt aagctcgggc cctgcagctc 
     2461 tagagctcga attctacagg tcactaatac catctaagta gttgattcat agtgactgca 
     2521 tatgttgtgt tttacagtat tatgtagtct gttttttatg caaaatctaa tttaatatat 
     2581 tgatatttat atcattttac gtttctcgtt caactttctt gtacaaagtg gctgtggata 
     2641 ttgaggtcca ggatctagag gagtttgcac tgaggccagc cccacaaggg atcaccatca 
     2701 aatgccgcat cactcgggac aagaagggga tggaccgcgg catgtacccc acctactttc 
     2761 tgcacctaga ccgtgaggat ggcaagaagg tgttcctcct ggcgggcagg aagagaaaga 
     2821 agagtaaaac ttccaattac ctcatctctg tggacccaac agacttgtct cggggaggcg 
     2881 atagctatat cgggaaattg cggtccaacc tgatgggcac caagttcacc gtttatgaca 
     2941 atggcgtcaa ccctcagaag gcatcctctt ccacgctgga aagcggaacc ttgcgccagg 
     3001 agctggcagc ggtgtgctat gagacaaatg tcctaggctt caagggacct cggaagatga 
     3061 gtgtgatcgt cccaggcatg aacatggttc atgagagagt ctgtatccgc ccccgcaatg 
     3121 aacatgagac cctgttagca cgctggcaga acaagaacac ggagagcatc attgagctgc 
     3181 agaacaagac gccagtctgg aatgatgaca cacagtccta tgtacttaac ttccacggcc 
     3241 gtgtcacaca ggcttctgtg aagaacttcc agatcatcca cggcaatgac ccggactaca 
     3301 tcgtcatgca gtttggccgg gtagcagaag atgtgttcac catggattac aactacccac 
     3361 tgtgtgcact gcaggccttt gccattgctc tgtccagctt tgacagcaag ctggcctgcg 
     3421 agtaggcaac ttta 
//
