LOCUS       pH7m34GW,0             10585 bp    DNA     circular      4-FEB-2008
SOURCE      
  ORGANISM  
COMMENT     http://www.informaxinc.com/
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|342095904|
COMMENT     VNTDBDATE|473164802|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pH7m34GW,0|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTAUTHOREML|manka@psb.ugent.be|
COMMENT     VNTOAUTHORNAME|Mansour Karimi|
COMMENT     VNTOAUTHORTEL|+3193313850|
COMMENT     VNTOAUTHOREML|mansour.karimi@psb.ugent.be|
FEATURES             Location/Qualifiers
     primer          complement(2051..2067)
                     /vntifkey="27"
                     /label=M13\R
     primer          complement(304..319)
                     /vntifkey="27"
                     /label=M13F
     misc_recomb     complement(1907..2031)
                     /vntifkey="86"
                     /label=attR4
     misc_feature    complement(453..1906)
                     /vntifkey="21"
                     /label=ccdB
     misc_recomb     complement(328..452)
                     /vntifkey="86"
                     /label=attR3
     terminator      21..246
                     /vntifkey="43"
                     /label=T35S
     misc_signal     2095..2294
                     /vntifkey="87"
                     /label=RB
     misc_marker     complement(7198..8447)
                     /vntifkey="22"
                     /label=Sm/SpR
     misc_signal     8453..8785
                     /vntifkey="87"
                     /label=LB
     misc_marker     complement(8789..10575)
                     /vntifkey="22"
                     /label=Hyg
BASE COUNT     2556 a      2813 c      2851 g      2365 t 
ORIGIN
        1 ctcccatatg gtcgacctgc aggtcactgg attttggttt taggaattag aaattttatt 
       61 gatagaagta ttttacaaat acaaatacat actaagggtt tcttatatgc tcaacacatg 
      121 agcgaaaccc tataagaacc ctaattccct tatctgggaa ctactcacac attattctgg 
      181 agaaaaatag agagagatag atttgtagag agagactggt gatttttgcg gactctagca 
      241 tggccgctcc cggccgccat ggccgcggct agtcagttag atcgttttcc cagtcacgac 
      301 gttgtaaaac gacggccagt gaattatcaa ctatgtataa taaagttgaa cgagaaacgt 
      361 aaaatgatat aaatatcaat atattaaatt agattttgca taaaaaacag actacataat 
      421 actgtaaaac acaacatatc cagtcactat ggcggccgca ttaggcaccc ccaggcttta 
      481 cactttatgc ttccggctcg tataatgtgt ggattttgag ttaggatccg tcgagatttt 
      541 caggagctaa ggaagctaaa atggagaaaa aaatcactgg atataccacc gttgatatat 
      601 cccaatggca tcgtaaagaa cattttgagg catttcagtc agttgctcaa tgtacctata 
      661 accagaccgt tcagctggat attacggcct ttttaaagac cgtaaagaaa aataagcaca 
      721 agttttatcc ggcctttatt cacattcttg cccgcctgat gaatgctcat ccggaattcc 
      781 gtatggcaat gaaagacggt gagctggtga tatgggatag tgttcaccct tgttacaccg 
      841 ttttccatga gcaaactgaa acgttttcat cgctctggag tgaataccac gacgatttcc 
      901 ggcagtttct acacatatat tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt 
      961 tccctaaagg gtttattgag aatatgtttt tcgtctcagc caatccctgg gtgagtttca 
     1021 ccagttttga tttaaacgtg gccaatatgg acaacttctt cgcccccgtt ttcaccatgg 
     1081 gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg 
     1141 ccgtttgtga tggcttccat gtcggcagaa tgcttaatga attacaacag tactgcgatg 
     1201 agtggcaggg cggggcgtaa tctagaggat ccggcttact aaaagccaga taacagtatg 
     1261 cgtatttgcg cgctgatttt tgcggtataa gaatatatac tgatatgtat acccgaagta 
     1321 tgtcaaaaag aggtatgcta tgaagcagcg tattacagtg acagttgaca gcgacagcta 
     1381 tcagttgctc aaggcatata tgatgtcaat atctccggtt cggtaagcac aaccatgcag 
     1441 aatgaagccc gtcgtctgcg tgccgaacgc tggaaagcgg aaaatcagga agggatggct 
     1501 gaggtcgccc ggtttattga aatgaacggc tcttttgccg acgagaacag gggctggtga 
     1561 aatgcagttt aaggtttaca cctataaaag agagagccgt tatcgtctgt ttgtggatgt 
     1621 acagagtgat attattgaca cgcccgggcg acggatggtg atccccctgg ccagtgcacg 
     1681 tctgctgtca gataaagtcc cccgtgaact ttacccggtg gtgcatatcg gggatgaaag 
     1741 ctggcgcatg atgaccaccg atatggccag tgtgccggtc tccgttatcg gggaagaagt 
     1801 ggctgatctc agccaccgcg aaaatgacat caaaaacgcc attaacctga tgttctgggg 
     1861 aatataaatg tcaggctccc ttatacacag ccagtctgca ggtcgaccat agtgactgga 
     1921 tatgttgtgt tttacagtat tatgtagtct gttttttatg caaaatctaa tttaatatat 
     1981 tgatatttat atcattttac gtttctcgtt caacttttct atacaaagtt gatagcttgg 
     2041 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcggg cccaagctta 
     2101 gcttgagctt ggatcagatt gtcgtttccc gccttcagtt taaactatca gtgtttgaca 
     2161 ggatatattg gcgggtaaac ctaagagaaa agagcgttta ttagaataac ggatatttaa 
     2221 aagggcgtga aaaggtttat ccgttcgtcc atttgtatgt gcatgccaac cacagggttc 
     2281 ccctcgggat caaagtactt tgatccaacc cctccgctgc tatagtgcag tcggcttctg 
     2341 acgttcagtg cagccgtctt ctgaaaacga catgtcgcac aagtcctaag ttacgcgaca 
     2401 ggctgccgcc ctgccctttt cctggcgttt tcttgtcgcg tgttttagtc gcataaagta 
     2461 gaatacttgc gactagaacc ggagacatta cgccatgaac aagagcgccg ccgctggcct 
     2521 gctgggctat gcccgcgtca gcaccgacga ccaggacttg accaaccaac gggccgaact 
     2581 gcacgcggcc ggctgcacca agctgttttc cgagaagatc accggcacca ggcgcgaccg 
     2641 cccggagctg gccaggatgc ttgaccacct acgccctggc gacgttgtga cagtgaccag 
     2701 gctagaccgc ctggcccgca gcacccgcga cctactggac attgccgagc gcatccagga 
     2761 ggccggcgcg ggcctgcgta gcctggcaga gccgtgggcc gacaccacca cgccggccgg 
     2821 ccgcatggtg ttgaccgtgt tcgccggcat tgccgagttc gagcgttccc taatcatcga 
     2881 ccgcacccgg agcgggcgcg aggccgccaa ggcccgaggc gtgaagtttg gcccccgccc 
     2941 taccctcacc ccggcacaga tcgcgcacgc ccgcgagctg atcgaccagg aaggccgcac 
     3001 cgtgaaagag gcggctgcac tgcttggcgt gcatcgctcg accctgtacc gcgcacttga 
     3061 gcgcagcgag gaagtgacgc ccaccgaggc caggcggcgc ggtgccttcc gtgaggacgc 
     3121 attgaccgag gccgacgccc tggcggccgc cgagaatgaa cgccaagagg aacaagcatg 
     3181 aaaccgcacc aggacggcca ggacgaaccg tttttcatta ccgaagagat cgaggcggag 
     3241 atgatcgcgg ccgggtacgt gttcgagccg cccgcgcacg tctcaaccgt gcggctgcat 
     3301 gaaatcctgg ccggtttgtc tgatgccaag ctggcggcct ggccggccag cttggccgct 
     3361 gaagaaaccg agcgccgccg tctaaaaagg tgatgtgtat ttgagtaaaa cagcttgcgt 
     3421 catgcggtcg ctgcgtatat gatgcgatga gtaaataaac aaatacgcaa ggggaacgca 
     3481 tgaaggttat cgctgtactt aaccagaaag gcgggtcagg caagacgacc atcgcaaccc 
     3541 atctagcccg cgccctgcaa ctcgccgggg ccgatgttct gttagtcgat tccgatcccc 
     3601 agggcagtgc ccgcgattgg gcggccgtgc gggaagatca accgctaacc gttgtcggca 
     3661 tcgaccgccc gacgattgac cgcgacgtga aggccatcgg ccggcgcgac ttcgtagtga 
     3721 tcgacggagc gccccaggcg gcggacttgg ctgtgtccgc gatcaaggca gccgacttcg 
     3781 tgctgattcc ggtgcagcca agcccttacg acatatgggc caccgccgac ctggtggagc 
     3841 tggttaagca gcgcattgag gtcacggatg gaaggctaca agcggccttt gtcgtgtcgc 
     3901 gggcgatcaa aggcacgcgc atcggcggtg aggttgccga ggcgctggcc gggtacgagc 
     3961 tgcccattct tgagtcccgt atcacgcagc gcgtgagcta cccaggcact gccgccgccg 
     4021 gcacaaccgt tcttgaatca gaacccgagg gcgacgctgc ccgcgaggtc caggcgctgg 
     4081 ccgctgaaat taaatcaaaa ctcatttgag ttaatgaggt aaagagaaaa tgagcaaaag 
     4141 cacaaacacg ctaagtgccg gccgtccgag cgcacgcagc agcaaggctg caacgttggc 
     4201 cagcctggca gacacgccag ccatgaagcg ggtcaacttt cagttgccgg cggaggatca 
     4261 caccaagctg aagatgtacg cggtacgcca aggcaagacc attaccgagc tgctatctga 
     4321 atacatcgcg cagctaccag agtaaatgag caaatgaata aatgagtaga tgaattttag 
     4381 cggctaaagg aggcggcatg gaaaatcaag aacaaccagg caccgacgcc gtggaatgcc 
     4441 ccatgtgtgg aggaacgggc ggttggccag gcgtaagcgg ctgggttgtc tgccggccct 
     4501 gcaatggcac tggaaccccc aagcccgagg aatcggcgtg acggtcgcaa accatccggc 
     4561 ccggtacaaa tcggcgcggc gctgggtgat gacctggtgg agaagttgaa ggccgcgcag 
     4621 gccgcccagc ggcaacgcat cgaggcagaa gcacgccccg gtgaatcgtg gcaagcggcc 
     4681 gctgatcgaa tccgcaaaga atcccggcaa ccgccggcag ccggtgcgcc gtcgattagg 
     4741 aagccgccca agggcgacga gcaaccagat tttttcgttc cgatgctcta tgacgtgggc 
     4801 acccgcgata gtcgcagcat catggacgtg gccgttttcc gtctgtcgaa gcgtgaccga 
     4861 cgagctggcg aggtgatccg ctacgagctt ccagacgggc acgtagaggt ttccgcaggg 
     4921 ccggccggca tggccagtgt gtgggattac gacctggtac tgatggcggt ttcccatcta 
     4981 accgaatcca tgaaccgata ccgggaaggg aagggagaca agcccggccg cgtgttccgt 
     5041 ccacacgttg cggacgtact caagttctgc cggcgagccg atggcggaaa gcagaaagac 
     5101 gacctggtag aaacctgcat tcggttaaac accacgcacg ttgccatgca gcgtacgaag 
     5161 aaggccaaga acggccgcct ggtgacggta tccgagggtg aagccttgat tagccgctac 
     5221 aagatcgtaa agagcgaaac cgggcggccg gagtacatcg agatcgagct agctgattgg 
     5281 atgtaccgcg agatcacaga aggcaagaac ccggacgtgc tgacggttca ccccgattac 
     5341 tttttgatcg atcccggcat cggccgtttt ctctaccgcc tggcacgccg cgccgcaggc 
     5401 aaggcagaag ccagatggtt gttcaagacg atctacgaac gcagtggcag cgccggagag 
     5461 ttcaagaagt tctgtttcac cgtgcgcaag ctgatcgggt caaatgacct gccggagtac 
     5521 gatttgaagg aggaggcggg gcaggctggc ccgatcctag tcatgcgcta ccgcaacctg 
     5581 atcgagggcg aagcatccgc cggttcctaa tgtacggagc agatgctagg gcaaattgcc 
     5641 ctagcagggg aaaaaggtcg aaaaggtctc tttcctgtgg atagcacgta cattgggaac 
     5701 ccaaagccgt acattgggaa ccggaacccg tacattggga acccaaagcc gtacattggg 
     5761 aaccggtcac acatgtaagt gactgatata aaagagaaaa aaggcgattt ttccgcctaa 
     5821 aactctttaa aacttattaa aactcttaaa acccgcctgg cctgtgcata actgtctggc 
     5881 cagcgcacag ccgaagagct gcaaaaagcg cctacccttc ggtcgctgcg ctccctacgc 
     5941 cccgccgctt cgcgtcggcc tatcgcggcc gctggccgct caaaaatggc tggcctacgg 
     6001 ccaggcaatc taccagggcg cggacaagcc gcgccgtcgc cactcgaccg ccggcgccca 
     6061 catcaaggca ccctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca 
     6121 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca 
     6181 gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga 
     6241 tagcggagtg tatactggct taactatgcg gcatcagagc agattgtact gagagtgcac 
     6301 catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct 
     6361 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca 
     6421 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac 
     6481 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt 
     6541 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg 
     6601 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc 
     6661 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc 
     6721 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc 
     6781 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac 
     6841 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt 
     6901 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct 
     6961 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc 
     7021 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt 
     7081 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg 
     7141 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc 
     7201 atgcatgata tatctcccaa tttgtgtagg gcttattatg cacgcttaaa aataataaaa 
     7261 gcagacttga cctgatagtt tggctgtgag caattatgtg cttagtgcat ctaatcgctt 
     7321 gagttaacgc cggcgaagcg gcgtcggctt gaacgaattt ctagctagac attatttgcc 
     7381 gactaccttg gtgatctcgc ctttcacgta gtggacaaat tcttccaact gatctgcgcg 
     7441 cgaggccaag cgatcttctt cttgtccaag ataagcctgt ctagcttcaa gtatgacggg 
     7501 ctgatactgg gccggcaggc gctccattgc ccagtcggca gcgacatcct tcggcgcgat 
     7561 tttgccggtt actgcgctgt accaaatgcg ggacaacgta agcactacat ttcgctcatc 
     7621 gccagcccag tcgggcggcg agttccatag cgttaaggtt tcatttagcg cctcaaatag 
     7681 atcctgttca ggaaccggat caaagagttc ctccgccgct ggacctacca aggcaacgct 
     7741 atgttctctt gcttttgtca gcaagatagc cagatcaatg tcgatcgtgg ctggctcgaa 
     7801 gatacctgca agaatgtcat tgcgctgcca ttctccaaat tgcagttcgc gcttagctgg 
     7861 ataacgccac ggaatgatgt cgtcgtgcac aacaatggtg acttctacag cgcggagaat 
     7921 ctcgctctct ccaggggaag ccgaagtttc caaaaggtcg ttgatcaaag ctcgccgcgt 
     7981 tgtttcatca agccttacgg tcaccgtaac cagcaaatca atatcactgt gtggcttcag 
     8041 gccgccatcc actgcggagc cgtacaaatg tacggccagc aacgtcggtt cgagatggcg 
     8101 ctcgatgacg ccaactacct ctgatagttg agtcgatact tcggcgatca ccgcttcccc 
     8161 catgatgttt aactttgttt tagggcgact gccctgctgc gtaacatcgt tgctgctcca 
     8221 taacatcaaa catcgaccca cggcgtaacg cgcttgctgc ttggatgccc gaggcataga 
     8281 ctgtacccca aaaaaacatg tcataacaag aagccatgaa aaccgccact gcgccgttac 
     8341 caccgctgcg ttcggtcaag gttctggacc agttgcgtga cggcagttac gctacttgca 
     8401 ttacagctta cgaaccgaac gaggcttatg tccactgggt tcgtgcccga attgatcaca 
     8461 ggcagcaacg ctctgtcatc gttacaatca acatgctacc ctccgcgaga tcatccgtgt 
     8521 ttcaaacccg gcagcttagt tgccgttctt ccgaatagca tcggtaacat gagcaaagtc 
     8581 tgccgcctta caacggctct cccgctgacg ccgtcccgga ctgatgggct gcctgtatcg 
     8641 agtggtgatt ttgtgccgag ctgccggtcg gggagctgtt ggctggctgg tggcaggata 
     8701 tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg gacgttttta 
     8761 atgtactgaa ttaacgccga attgaattat cagcttgcat gccggtcgat ctagtaacat 
     8821 agatgacacc gcgcgcgata atttatccta gtttgcgcgc tatattttgt tttctatcgc 
     8881 gtattaaatg tataattgcg ggactctaat cataaaaacc catctcataa ataacgtcat 
     8941 gcattacatg ttaattatta catgcttaac gtaattcaac agaaattata tgataatcat 
     9001 cgcaagaccg gcaacaggat tcaatcttaa gaaactttat tgccaaatgt ttgaacgatc 
     9061 tgcttgactc tagggaatta attcctgaat cactgcgacc ggccctcccg cgacccagcc 
     9121 gagcgagctt agcgaactgt ggacgagaac tgtgccacca agcgtaaggc cgttctctcg 
     9181 catttgcctt gctaggctcg cgcgagttgc tggctgaggc gttctcgaaa tcagctcttg 
     9241 ttcggtcggc atctactcta ttcctttgcc ctcggacgag tgctggggcg tcggtttcca 
     9301 ctatcggcga gtacttctac acagccatcg gtccagacgg ccgcgcttct gcgggcgatt 
     9361 tgtgtacgcc cgacagtccc ggctccggat cggacgattg cgtcgcatcg accctgcgcc 
     9421 caagctgcat catcgaaatt gccgtcaacc aagctctgat agagttggtc aagaccaatg 
     9481 cggagcatat acgcccggag ccttggcgat cctgcaagct ccggatgcct ccgctcgaag 
     9541 tagcgcgtct gctgctccat acaagccaac cacggcctcc agaagaagat gttggcgacc 
     9601 tcgtattggg aatccccgaa catcgcctcg ctccagtcaa tgaccgctgt tatgcggcca 
     9661 ttgtccgtca ggacattgtt ggagccgaat ccgcgtgcac gagtgccgga cttcggggca 
     9721 gtcctcgccc aaagcatcag ctcatcgaga gcctgcgcga cggacgcact gacggtgtcg 
     9781 tccatcacag tttgccagtg atacacatgg ggatcagcaa tcgcgcatat gaaatcacgc 
     9841 catgtagtgt attgaccgat tccttgcggt ccgaatgggc cgaacccgct cgtctggcta 
     9901 agatcggccg cagcgatagc atccatagcc tccgcgaccg gctgaagaac agcgggcagt 
     9961 tcggtttcag gcaggtcttg caacgtgaca ccctgtgcac ggcgggagat gcaataggtc 
    10021 aggctctcgc tgaactcccc aatgtcaagc acttccggaa tcgggagcgc ggccgatgca 
    10081 aagtgccgat aaacataacg atctttgtag aaaccatcgg cgcagctatt tacccgcagg 
    10141 acatatccac gccctcctac atcgaagctg aaagcacgag attcttcgcc ctccgagagc 
    10201 tgcatcaggt cggagacgct gtcgaacttt tcgatcagaa acttctcgac agacgtcgcg 
    10261 gtgagttcag gctttttcat aggggggatt cgagttgaga gtgaatatga gactctaatt 
    10321 ggataccgag gggaatttat ggaacgtcag tggagcattt ttgacaagaa atatttgcta 
    10381 gctgatagtg accttaggcg acttttgaac gcgcaataat ggtttctgac gtatgtgctt 
    10441 agctcattaa actccagaaa cccgcggctc agtggctcct tcaacgttgc ggttctgtca 
    10501 gttccaaacg taaaacggct tgtcccgcgt catcggcggg gtcataacgt gactccctta 
    10561 attctcatgt atgataattc gagct 
//
