LOCUS       UBQ10prom/pDONRP        4632 bp    DNA     circular      5-FEB-2008
DEFINITION  attL4-attR1.
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     VNTDATE|473263309|
COMMENT     VNTDBDATE|473263309|
COMMENT     LSOWNER|
COMMENT     VNTNAME|UBQ10prom/pDONRP4-P1R|
COMMENT     VNTAUTHORNAME|Demo User|
FEATURES             Location/Qualifiers
     misc_feature    2540..2635
                     /vntifkey="21"
                     /label=attL4
     misc_feature    4625..116
                     /vntifkey="21"
                     /label=attR1
     terminator      2215..2242
                     /invitrogen="2590000"
                     /vntifkey="43"
                     /label=rrnB\T2\transcription\terminator
     terminator      2374..2417
                     /invitrogen="2580000"
                     /vntifkey="43"
                     /label=rrnB\T1\transcription\terminator
     promoter        complement(191..207)
                     /invitrogen="2370000"
                     /vntifkey="30"
                     /label=T7\promoter
     primer          complement(188..207)
                     /invitrogen="2100000"
                     /vntifkey="27"
                     /label=T7\primer
     primer          complement(212..228)
                     /invitrogen="2010000"
                     /vntifkey="27"
                     /label=M13\reverse\primer
     primer          2464..2480
                     /invitrogen="2000000"
                     /vntifkey="27"
                     /label=M13\(-40)\forward\primer
     primer          2484..2499
                     /invitrogen="1980000"
                     /vntifkey="27"
                     /label=M13\(-20)\forward\primer
     CDS             341..1150
                     /invitrogen="1210000"
                     /vntifkey="4"
                     /label=Kan(R)
     promoter        2638..4234
                     /vntifkey="29"
                     /label=5'\Promoter\region
     intron          4320..4623
                     /vntifkey="15"
                     /label=Intron\1.1
     misc_feature    4235..4319
                     /vntifkey="21"
                     /label=Exon\1.1
     5'UTR           4124..4623
                     /vntifkey="52"
                     /label=5'UTR
BASE COUNT     1297 a      1045 c       944 g      1346 t 
ORIGIN
        1 tacaaaaaag ttgaacgaga aacgtaaaat gatataaata tcaatatatt aaattagatt 
       61 ttgcataaaa aacagactac ataatactgt aaaacacaac atatgcagtc actatgaatc 
      121 aactacttag atggtattag tgacctgtag aattcgagct ctagagctgc agggcggccg 
      181 cgatatcccc tatagtgagt cgtattacat ggtcatagct gtttcctggc agctctggcc 
      241 cgtgtctcaa aatctctgat gttacattgc acaagataaa aatatatcat catgaacaat 
      301 aaaactgtct gcttacataa acagtaatac aaggggtgtt atgagccata ttcaacggga 
      361 aacgtcgagg ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc 
      421 tcgcgataat gtcgggcaat caggtgcgac aatctatcgc ttgtatggga agcccgatgc 
      481 gccagagttg tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat 
      541 ggtcagacta aactggctga cggaatttat gcctcttccg accatcaagc attttatccg 
      601 tactcctgat gatgcatggt tactcaccac tgcgatcccc ggaaaaacag cattccaggt 
      661 attagaagaa tatcctgatt caggtgaaaa tattgttgat gcgctggcag tgttcctgcg 
      721 ccggttgcat tcgattcctg tttgtaattg tccttttaac agcgatcgcg tatttcgtct 
      781 cgctcaggcg caatcacgaa tgaataacgg tttggttgat gcgagtgatt ttgatgacga 
      841 gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg cataaacttt tgccattctc 
      901 accggattca gtcgtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg 
      961 gaaattaata ggttgtattg atgttggacg agtcggaatc gcagaccgat accaggatct 
     1021 tgccatccta tggaactgcc tcggtgagtt ttctccttca ttacagaaac ggctttttca 
     1081 aaaatatggt attgataatc ctgatatgaa taaattgcag tttcatttga tgctcgatga 
     1141 gtttttctaa tcagaattgg ttaattggtt gtaacactgg cagagcatta cgctgacttg 
     1201 acgggacggc gcaagctcat gaccaaaatc ccttaacgtg agttacgcgt cgttccactg 
     1261 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt 
     1321 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca 
     1381 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac 
     1441 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac 
     1501 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct 
     1561 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg 
     1621 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca 
     1681 gcgtgagcat tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt 
     1741 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta 
     1801 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc 
     1861 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc 
     1921 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa 
     1981 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag 
     2041 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg 
     2101 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga 
     2161 gcgcaacgca attaatacgc gtaccgctag ccaggaagag tttgtagaaa cgcaaaaagg 
     2221 ccatccgtca ggatggcctt ctgcttagtt tgatgcctgg cagtttatgg cgggcgtcct 
     2281 gcccgccacc ctccgggccg ttgcttcaca acgttcaaat ccgctcccgg cggatttgtc 
     2341 ctactcagga gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtcttccgac 
     2401 tgagcctttc gttttatttg atgcctggca gttccctact ctcgcgttaa cgctagcatg 
     2461 gatgttttcc cagtcacgac gttgtaaaac gacggccagt cttaagctcg ggcccgcgtt 
     2521 aacgctacca tggagctcca aataatgatt ttattttgac tgatagtgac ctgttcgttg 
     2581 caacaaattg ataagcaatg cttttttata atgccaactt tgtatagaaa agttgctagt 
     2641 ctagctcaac agagctttta acccaaattg gtacaataga atacaacttt agatcataat 
     2701 tctcaaaaga aagagattcc ttagctattc tatctgccac tccatttcct tctcggcttg 
     2761 tatgcacaag cataaaatcc tcaaacttgc taagtagata ctttatgtct tggataattg 
     2821 gattgagact tgacaagcat aactttcatg taaccaaaga cacaagttgc tgagaatcca 
     2881 cctcaaaaat gatcttccta taattgaatc gggataatga cagcacagcc catctaagag 
     2941 cctccacttc tacttccagc acgcttctta cttttaccac agctcttgca cctaaccata 
     3001 acaccttccc tgtatgatcg cgaagcaccc accctaagcc acattttaat ccttctgttg 
     3061 gccatgcccc atcaaagttg cacttaaccc aagattgtgg tggagcttcc catgtttctc 
     3121 gtctgtcccg acggtgttgt ggttggtgct ttccttacat tctgagcctc tttccttcta 
     3181 atccactcat ctgcatcttc ttgtgtcctt actaatacct cattggttcc aaattccctc 
     3241 cctttaagca ccagctcgtt tctgttcttc cacagcctcc caagtatcca agggactaaa 
     3301 gcctccacat tcttcagatc aggatattct tgtttaagat gttgaactct atggaggttt 
     3361 gtatgaactg atgatctagg accggataag ttcccttctt catagcgaac ttattcaaag 
     3421 aatgttttgt gtatcattct tgttacattg ttattaatga aaaaatatta ttggtcattg 
     3481 gactgaacac gagtgttaaa tatggaccag gccccaaata agatccattg atatatgaat 
     3541 taaataacaa gaataaatcg agtcaccaaa ccacttgcct tttttaacga gacttgttca 
     3601 ccaacttgat acaaaagtca ttatcctatg caaatcaata atcatacaaa aatatccaat 
     3661 aacactaaaa aattaaaaga aatggataat ttcacaatat gttatacgat aaagaagtta 
     3721 cttttccaag aaattcactg attttataag cccacttgca ttagataaat ggcaaaaaaa 
     3781 aacaaaaagg aaaagaaata aagcacgaag aattctagaa aatacgaaat acgcttcaat 
     3841 gcagtgggac ccacggttca attattgcca attttcagct ccaccgtata tttaaaaaat 
     3901 aaaacgataa tgctaaaaaa atataaatcg taacgatcgt taaatctcaa cggctggatc 
     3961 ttatgacgac cgttagaaat tgtggttgtc gacgagtcag taataaacgg cgtcaaagtg 
     4021 gttgcagccg gcacacacga gtcgtgttta tcaactcaaa gcacaaatac ttttcctcaa 
     4081 cctaaaaata aggcaattag ccaaaaacaa ctttgcgtgt aaacaacgct caatacacgt 
     4141 gtcattttat tattagctat tgcttcaccg ccttagcttt ctcgtgacct agtcgtcctc 
     4201 gtcttttctt cttcttcttc tataaaacaa tacccaaaga gctcttcttc ttcacaattc 
     4261 agatttcaat ttctcaaaat cttaaaaact ttctctcaat tctctctacc gtgatcaagg 
     4321 taaatttctg tgttccttat tctctcaaaa tcttcgattt tgttttcgtt cgatcccaat 
     4381 ttcgtatatg ttctttggtt tagattctgt taatcttaga tcgaagacga ttttctgggt 
     4441 ttgatcgtta gatatcatct taattctcga ttagggtttc atagatatca tccgatttgt 
     4501 tcaaataatt tgagttttgt cgaataatta ctcttcgatt tgtgatttct atctagatct 
     4561 ggtgttagtt tctagtttgt gcgatcgaat ttgtcgatta atctgagttt ttctgattaa 
     4621 caggcaagtt tg 
//
