LOCUS 2xPH\EVECTIN-2wSTOP(GIBSON)/pDONRP2R-P3\(p3') 3323 bp DNA circular 18-NOV-2013 DEFINITION attL3-attR2. SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT VNTDATE|678987823| COMMENT VNTDBDATE|678989057| COMMENT LSOWNER| COMMENT VNTNAME|2xPH EVECTIN-2wSTOP(GIBSON)/pDONRP2R-P3 (p3')| COMMENT VNTAUTHORNAME|RDP-Z400-SI| FEATURES Location/Qualifiers terminator 2152..2179 /invitrogen="2590000" /vntifkey="43" /label=rrnB\T2\transcription\terminator terminator 2311..2354 /invitrogen="2580000" /vntifkey="43" /label=rrnB\T1\transcription\terminator promoter complement(128..144) /invitrogen="2370000" /vntifkey="30" /label=T7\promoter primer complement(125..144) /invitrogen="2100000" /vntifkey="27" /label=T7\primer primer complement(149..165) /invitrogen="2010000" /vntifkey="27" /label=M13\reverse\primer primer 2401..2417 /invitrogen="2000000" /vntifkey="27" /label=M13\(-40)\forward\primer primer 2421..2436 /invitrogen="1980000" /vntifkey="27" /label=M13\(-20)\forward\primer CDS 278..1087 /invitrogen="1210000" /vntifkey="4" /label=Kan(R) misc_feature 2637..2966 /vntifkey="21" /label=PH\domain\EVICTIN2 misc_feature complement(3316..88) /vntifkey="21" /label=attL3 misc_feature complement(2508..2631) /vntifkey="21" /label=attR2 misc_feature 2967..2978 /vntifkey="21" /label=Linker misc_feature 2979..3308 /vntifkey="21" /label=PH\domain\EVECTIN2 misc_feature 2967..2978 /vntifkey="21" /label=Linker BASE COUNT 857 a 756 c 829 g 881 t ORIGIN 1 ttatacaaag ttggcattat aaaaaagcat tgcttatcaa tttgttgcaa cgaacaggtc 61 actatcagtc aaaataaaat cattatttgg agctccatgg tagcgttaac gcggccgcga 121 tatcccctat agtgagtcgt attacatggt catagctgtt tcctggcagc tctggcccgt 181 gtctcaaaat ctctgatgtt acattgcaca agataaaaat atatcatcat gaacaataaa 241 actgtctgct tacataaaca gtaatacaag gggtgttatg agccatattc aacgggaaac 301 gtcgaggccg cgattaaatt ccaacatgga tgctgattta tatgggtata aatgggctcg 361 cgataatgtc gggcaatcag gtgcgacaat ctatcgcttg tatgggaagc ccgatgcgcc 421 agagttgttt ctgaaacatg gcaaaggtag cgttgccaat gatgttacag atgagatggt 481 cagactaaac tggctgacgg aatttatgcc tcttccgacc atcaagcatt ttatccgtac 541 tcctgatgat gcatggttac tcaccactgc gatccccgga aaaacagcat tccaggtatt 601 agaagaatat cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg 661 gttgcattcg attcctgttt gtaattgtcc ttttaacagc gatcgcgtat ttcgtctcgc 721 tcaggcgcaa tcacgaatga ataacggttt ggttgatgcg agtgattttg atgacgagcg 781 taatggctgg cctgttgaac aagtctggaa agaaatgcat aaacttttgc cattctcacc 841 ggattcagtc gtcactcatg gtgatttctc acttgataac cttatttttg acgaggggaa 901 attaataggt tgtattgatg ttggacgagt cggaatcgca gaccgatacc aggatcttgc 961 catcctatgg aactgcctcg gtgagttttc tccttcatta cagaaacggc tttttcaaaa 1021 atatggtatt gataatcctg atatgaataa attgcagttt catttgatgc tcgatgagtt 1081 tttctaatca gaattggtta attggttgta acactggcag agcattacgc tgacttgacg 1141 ggacggcgca agctcatgac caaaatccct taacgtgagt tacgcgtcgt tccactgagc 1201 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat 1261 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga 1321 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt 1381 ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata 1441 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac 1501 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg 1561 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg 1621 tgagcattga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag 1681 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct 1741 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc 1801 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt 1861 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg 1921 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga 1981 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg 2041 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg 2101 caacgcaatt aatacgcgta ccgctagcca ggaagagttt gtagaaacgc aaaaaggcca 2161 tccgtcagga tggccttctg cttagtttga tgcctggcag tttatggcgg gcgtcctgcc 2221 cgccaccctc cgggccgttg cttcacaacg ttcaaatccg ctcccggcgg atttgtccta 2281 ctcaggagag cgttcaccga caaacaacag ataaaacgaa aggcccagtc ttccgactga 2341 gcctttcgtt ttatttgatg cctggcagtt ccctactctc gcgttaacgc tagcatggat 2401 gttttcccag tcacgacgtt gtaaaacgac ggccagtctt aagctcgggc cctgcagctc 2461 tagagctcga attctacagg tcactaatac catctaagta gttgattcat agtgactgca 2521 tatgttgtgt tttacagtat tatgtagtct gttttttatg caaaatctaa tttaatatat 2581 tgatatttat atcattttac gtttctcgtt caactttctt gtacaaagtg gctaccatgg 2641 cgtttgtgaa gagtggctgg ttgctgcgac agagtactat tttgaagcgc tggaagaaga 2701 actggtttga tctgtggtcg gatggtcacc tgatctatta tgatgaccag actcggcaga 2761 atatcgagga taaggtccac atgccaatgg actgcatcaa catccgcacg gggcaggaat 2821 gtcgggatac tcagcccccg gatggaaagt caaaagactg catgctccag attgtttgtc 2881 gagatgggaa aacaattagt ctttgtgcag aaagcacaga tgattgcttg gcctggaaat 2941 ttacactcca agattctagg acaaacgtcg acggtaccat ggcgtttgtg aagagtggct 3001 ggttgctgcg acagagtact attttgaagc gctggaagaa gaactggttt gatctgtggt 3061 cggatggtca cctgatctat tatgatgacc agactcggca gaatatcgag gataaggtcc 3121 acatgccaat ggactgcatc aacatccgca cggggcagga atgtcgggat actcagcccc 3181 cggatggaaa gtcaaaagac tgcatgctcc agattgtttg tcgagatggg aaaacaatta 3241 gtctttgtgc agaaagcaca gatgattgct tggcctggaa atttacactc caagattcta 3301 ggacaaacta gctagcaact tta //