LOCUS UBQ10prom/pDONRP4-P1R 4632 bp DNA circular 5-FEB-2008 DEFINITION attL4-attR1. SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT VNTDATE|473263309| COMMENT VNTDBDATE|473263309| COMMENT LSOWNER| COMMENT VNTNAME|UBQ10prom/pDONRP4-P1R| COMMENT VNTAUTHORNAME|RDP-Z400-SI| FEATURES Location/Qualifiers misc_feature 2540..2635 /vntifkey="21" /label=attL4 misc_feature 4625..116 /vntifkey="21" /label=attR1 terminator 2215..2242 /invitrogen="2590000" /vntifkey="43" /label=rrnB\T2\transcription\terminator terminator 2374..2417 /invitrogen="2580000" /vntifkey="43" /label=rrnB\T1\transcription\terminator promoter complement(191..207) /invitrogen="2370000" /vntifkey="30" /label=T7\promoter primer complement(188..207) /invitrogen="2100000" /vntifkey="27" /label=T7\primer primer complement(212..228) /invitrogen="2010000" /vntifkey="27" /label=M13\reverse\primer primer 2464..2480 /invitrogen="2000000" /vntifkey="27" /label=M13\(-40)\forward\primer primer 2484..2499 /invitrogen="1980000" /vntifkey="27" /label=M13\(-20)\forward\primer CDS 341..1150 /invitrogen="1210000" /vntifkey="4" /label=Kan(R) promoter 2638..4234 /vntifkey="29" /label=5'\Promoter\region intron 4320..4623 /vntifkey="15" /label=Intron\1.1 misc_feature 4235..4319 /vntifkey="21" /label=Exon\1.1 5'UTR 4124..4623 /vntifkey="52" /label=5'UTR BASE COUNT 1297 a 1045 c 944 g 1346 t ORIGIN 1 tacaaaaaag ttgaacgaga aacgtaaaat gatataaata tcaatatatt aaattagatt 61 ttgcataaaa aacagactac ataatactgt aaaacacaac atatgcagtc actatgaatc 121 aactacttag atggtattag tgacctgtag aattcgagct ctagagctgc agggcggccg 181 cgatatcccc tatagtgagt cgtattacat ggtcatagct gtttcctggc agctctggcc 241 cgtgtctcaa aatctctgat gttacattgc acaagataaa aatatatcat catgaacaat 301 aaaactgtct gcttacataa acagtaatac aaggggtgtt atgagccata ttcaacggga 361 aacgtcgagg ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc 421 tcgcgataat gtcgggcaat caggtgcgac aatctatcgc ttgtatggga agcccgatgc 481 gccagagttg tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat 541 ggtcagacta aactggctga cggaatttat gcctcttccg accatcaagc attttatccg 601 tactcctgat gatgcatggt tactcaccac tgcgatcccc ggaaaaacag cattccaggt 661 attagaagaa tatcctgatt caggtgaaaa tattgttgat gcgctggcag tgttcctgcg 721 ccggttgcat tcgattcctg tttgtaattg tccttttaac agcgatcgcg tatttcgtct 781 cgctcaggcg caatcacgaa tgaataacgg tttggttgat gcgagtgatt ttgatgacga 841 gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg cataaacttt tgccattctc 901 accggattca gtcgtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg 961 gaaattaata ggttgtattg atgttggacg agtcggaatc gcagaccgat accaggatct 1021 tgccatccta tggaactgcc tcggtgagtt ttctccttca ttacagaaac ggctttttca 1081 aaaatatggt attgataatc ctgatatgaa taaattgcag tttcatttga tgctcgatga 1141 gtttttctaa tcagaattgg ttaattggtt gtaacactgg cagagcatta cgctgacttg 1201 acgggacggc gcaagctcat gaccaaaatc ccttaacgtg agttacgcgt cgttccactg 1261 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt 1321 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca 1381 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac 1441 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac 1501 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct 1561 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg 1621 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca 1681 gcgtgagcat tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt 1741 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta 1801 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc 1861 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc 1921 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa 1981 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag 2041 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg 2101 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga 2161 gcgcaacgca attaatacgc gtaccgctag ccaggaagag tttgtagaaa cgcaaaaagg 2221 ccatccgtca ggatggcctt ctgcttagtt tgatgcctgg cagtttatgg cgggcgtcct 2281 gcccgccacc ctccgggccg ttgcttcaca acgttcaaat ccgctcccgg cggatttgtc 2341 ctactcagga gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtcttccgac 2401 tgagcctttc gttttatttg atgcctggca gttccctact ctcgcgttaa cgctagcatg 2461 gatgttttcc cagtcacgac gttgtaaaac gacggccagt cttaagctcg ggcccgcgtt 2521 aacgctacca tggagctcca aataatgatt ttattttgac tgatagtgac ctgttcgttg 2581 caacaaattg ataagcaatg cttttttata atgccaactt tgtatagaaa agttgctagt 2641 ctagctcaac agagctttta acccaaattg gtacaataga atacaacttt agatcataat 2701 tctcaaaaga aagagattcc ttagctattc tatctgccac tccatttcct tctcggcttg 2761 tatgcacaag cataaaatcc tcaaacttgc taagtagata ctttatgtct tggataattg 2821 gattgagact tgacaagcat aactttcatg taaccaaaga cacaagttgc tgagaatcca 2881 cctcaaaaat gatcttccta taattgaatc gggataatga cagcacagcc catctaagag 2941 cctccacttc tacttccagc acgcttctta cttttaccac agctcttgca cctaaccata 3001 acaccttccc tgtatgatcg cgaagcaccc accctaagcc acattttaat ccttctgttg 3061 gccatgcccc atcaaagttg cacttaaccc aagattgtgg tggagcttcc catgtttctc 3121 gtctgtcccg acggtgttgt ggttggtgct ttccttacat tctgagcctc tttccttcta 3181 atccactcat ctgcatcttc ttgtgtcctt actaatacct cattggttcc aaattccctc 3241 cctttaagca ccagctcgtt tctgttcttc cacagcctcc caagtatcca agggactaaa 3301 gcctccacat tcttcagatc aggatattct tgtttaagat gttgaactct atggaggttt 3361 gtatgaactg atgatctagg accggataag ttcccttctt catagcgaac ttattcaaag 3421 aatgttttgt gtatcattct tgttacattg ttattaatga aaaaatatta ttggtcattg 3481 gactgaacac gagtgttaaa tatggaccag gccccaaata agatccattg atatatgaat 3541 taaataacaa gaataaatcg agtcaccaaa ccacttgcct tttttaacga gacttgttca 3601 ccaacttgat acaaaagtca ttatcctatg caaatcaata atcatacaaa aatatccaat 3661 aacactaaaa aattaaaaga aatggataat ttcacaatat gttatacgat aaagaagtta 3721 cttttccaag aaattcactg attttataag cccacttgca ttagataaat ggcaaaaaaa 3781 aacaaaaagg aaaagaaata aagcacgaag aattctagaa aatacgaaat acgcttcaat 3841 gcagtgggac ccacggttca attattgcca attttcagct ccaccgtata tttaaaaaat 3901 aaaacgataa tgctaaaaaa atataaatcg taacgatcgt taaatctcaa cggctggatc 3961 ttatgacgac cgttagaaat tgtggttgtc gacgagtcag taataaacgg cgtcaaagtg 4021 gttgcagccg gcacacacga gtcgtgttta tcaactcaaa gcacaaatac ttttcctcaa 4081 cctaaaaata aggcaattag ccaaaaacaa ctttgcgtgt aaacaacgct caatacacgt 4141 gtcattttat tattagctat tgcttcaccg ccttagcttt ctcgtgacct agtcgtcctc 4201 gtcttttctt cttcttcttc tataaaacaa tacccaaaga gctcttcttc ttcacaattc 4261 agatttcaat ttctcaaaat cttaaaaact ttctctcaat tctctctacc gtgatcaagg 4321 taaatttctg tgttccttat tctctcaaaa tcttcgattt tgttttcgtt cgatcccaat 4381 ttcgtatatg ttctttggtt tagattctgt taatcttaga tcgaagacga ttttctgggt 4441 ttgatcgtta gatatcatct taattctcga ttagggtttc atagatatca tccgatttgt 4501 tcaaataatt tgagttttgt cgaataatta ctcttcgatt tgtgatttct atctagatct 4561 ggtgttagtt tctagtttgt gcgatcgaat ttgtcgatta atctgagttt ttctgattaa 4621 caggcaagtt tg //