LOCUS UBQ10prom/mCITRINEnoSTOP/2xPH\EVECTIN-2wSTOP/pB7m34GW,0\rc 11727 bp DNA circular 20-DEC-2013 DEFINITION attB1-attB2-attB3-attB4. SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT VNTDATE|681908465| COMMENT VNTDBDATE|681908465| COMMENT LSOWNER| COMMENT VNTNAME|UBQ10prom/mCITRINEnoSTOP/2xPH EVECTIN-2wSTOP/pB7m34GW,0 rc| COMMENT VNTAUTHORNAME|RDP-Z400-SI| FEATURES Location/Qualifiers misc_feature 10265..10285 /vntifkey="21" /label=attB1 misc_feature complement(11015..11035) /vntifkey="21" /label=attB2 misc_feature complement(11720..13) /vntifkey="21" /label=attB3 misc_feature 8255..8275 /vntifkey="21" /label=attB4 primer 8219..8235 /vntifkey="27" /label=M13\R primer 22..37 /vntifkey="27" /label=M13F terminator complement(95..320) /vntifkey="43" /label=T35S misc_signal complement(7992..8191) /vntifkey="87" /label=RB misc_marker 1839..3088 /vntifkey="22" /label=Sm/SpR misc_signal complement(1501..1833) /vntifkey="87" /label=LB misc_marker 350..1497 /vntifkey="22" /label=Bar /note="Pnos-Bar-Tnos, Plant selectable marker" 5'UTR 9764..10263 /vntifkey="52" /label=5'UTR misc_feature 9875..9959 /vntifkey="21" /label=Exon\1.1 intron 9960..10263 /vntifkey="15" /label=Intron\1.1 promoter 8278..9874 /vntifkey="29" /label=5'\Promoter\region CDS 10294..11010 /vntifkey="4" /label=mCITRINE misc_feature 11371..11382 /vntifkey="21" /label=Linker misc_feature 11383..11712 /vntifkey="21" /label=PH\domain\EVECTIN2 misc_feature 11041..11370 /vntifkey="21" /label=PH\domain\EVICTIN2 BASE COUNT 2738 a 3147 c 2989 g 2853 t ORIGIN 1 ttatacatag ttgataattc actggccgtc gttttacaac gtcgtgactg ggaaaacgat 61 ctaactgact agccgcggcc atggcggccg ggagcggcca tgctagagtc cgcaaaaatc 121 accagtctct ctctacaaat ctatctctct ctatttttct ccagaataat gtgtgagtag 181 ttcccagata agggaattag ggttcttata gggtttcgct catgtgttga gcatataaga 241 aacccttagt atgtatttgt atttgtaaaa tacttctatc aataaaattt ctaattccta 301 aaaccaaaat ccagtgacct gcaggtcgac catatgggag agctcgaatt atcatacatg 361 agaattaagg gagtcacgtt atgacccccg ccgatgacgc gggacaagcc gttttacgtt 421 tggaactgac agaaccgcaa cgttgaagga gccactgagc cgcgggtttc tggagtttaa 481 tgagctaagc acatacgtca gaaaccatta ttgcgcgttc aaaagtcgcc taaggtcact 541 atcagctagc aaatatttct tgtcaaaaat gctccactga cgttccataa attcccctcg 601 gtatccaatt agagtctcat attcactctc aactcgatcg aggggatcta ccatgagccc 661 agaacgacgc ccggccgaca tccgccgtgc caccgaggcg gacatgccgg cggtctgcac 721 catcgtcaac cactacatcg agacaagcac ggtcaacttc cgtaccgagc cgcaggaacc 781 gcaggagtgg acggacgacc tcgtccgtct gcgggagcgc tatccctggc tcgtcgccga 841 ggtggacggc gaggtcgccg gcatcgccta cgcgggtccc tggaaggcac gcaacgccta 901 cgactggacg gccgagtcga ccgtgtacgt ctccccccgc caccagcgga cgggactggg 961 ctccacgctc tacacccacc tgctgaagtc cctggaggca cagggcttca agagcgtggt 1021 cgctgtcatc gggctgccca acgacccgag cgtgcgcatg cacgaggcgc tcggatatgc 1081 cccccgcggc atgctgcggg cggccggctt caagcacggg aactggcatg acgtgggttt 1141 ctggcagctg gacttcagcc tgccggtgcc gccccgtccg gtcctgcccg tcaccgaaat 1201 ctgatgaccc ctagagtcaa gcagatcgtt caaacatttg gcaataaagt ttcttaagat 1261 tgaatcctgt tgccggtctt gcgatgatta tcatataatt tctgttgaat tacgttaagc 1321 atgtaataat taacatgtaa tgcatgacgt tatttatgag atgggttttt atgattagag 1381 tcccgcaatt atacatttaa tacgcgatag aaaacaaaat atagcgcgca aactaggata 1441 aattatcgcg cgcggtgtca tctatatgtt actagatcga ccggcatgca agctgataat 1501 tcaattcggc gttaattcag tacattaaaa acgtccgcaa tgtgttatta agttgtctaa 1561 gcgtcaattt gtttacacca caatatatcc tgccaccagc cagccaacag ctccccgacc 1621 ggcagctcgg cacaaaatca ccactcgata caggcagccc atcagtccgg gacggcgtca 1681 gcgggagagc cgttgtaagg cggcagactt tgctcatgtt accgatgcta ttcggaagaa 1741 cggcaactaa gctgccgggt ttgaaacacg gatgatctcg cggagggtag catgttgatt 1801 gtaacgatga cagagcgttg ctgcctgtga tcaattcggg cacgaaccca gtggacataa 1861 gcctcgttcg gttcgtaagc tgtaatgcaa gtagcgtaac tgccgtcacg caactggtcc 1921 agaaccttga ccgaacgcag cggtggtaac ggcgcagtgg cggttttcat ggcttcttgt 1981 tatgacatgt ttttttgggg tacagtctat gcctcgggca tccaagcagc aagcgcgtta 2041 cgccgtgggt cgatgtttga tgttatggag cagcaacgat gttacgcagc agggcagtcg 2101 ccctaaaaca aagttaaaca tcatggggga agcggtgatc gccgaagtat cgactcaact 2161 atcagaggta gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt 2221 gtacggctcc gcagtggatg gcggcctgaa gccacacagt gatattgatt tgctggttac 2281 ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg atcaacgacc ttttggaaac 2341 ttcggcttcc cctggagaga gcgagattct ccgcgctgta gaagtcacca ttgttgtgca 2401 cgacgacatc attccgtggc gttatccagc taagcgcgaa ctgcaatttg gagaatggca 2461 gcgcaatgac attcttgcag gtatcttcga gccagccacg atcgacattg atctggctat 2521 cttgctgaca aaagcaagag aacatagcgt tgccttggta ggtccagcgg cggaggaact 2581 ctttgatccg gttcctgaac aggatctatt tgaggcgcta aatgaaacct taacgctatg 2641 gaactcgccg cccgactggg ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat 2701 ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat 2761 ggagcgcctg ccggcccagt atcagcccgt catacttgaa gctagacagg cttatcttgg 2821 acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg tccactacgt 2881 gaaaggcgag atcaccaagg tagtcggcaa ataatgtcta gctagaaatt cgttcaagcc 2941 gacgccgctt cgccggcgtt aactcaagcg attagatgca ctaagcacat aattgctcac 3001 agccaaacta tcaggtcaag tctgctttta ttatttttaa gcgtgcataa taagccctac 3061 acaaattggg agatatatca tgcatgacca aaatccctta acgtgagttt tcgttccact 3121 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg 3181 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc 3241 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata 3301 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta 3361 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc 3421 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg 3481 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac 3541 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg 3601 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt 3661 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct 3721 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg 3781 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata 3841 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca 3901 gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc 3961 tgtgcggtat ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat 4021 agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca 4081 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag 4141 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa 4201 acgcgcgagg cagggtgcct tgatgtgggc gccggcggtc gagtggcgac ggcgcggctt 4261 gtccgcgccc tggtagattg cctggccgta ggccagccat ttttgagcgg ccagcggccg 4321 cgataggccg acgcgaagcg gcggggcgta gggagcgcag cgaccgaagg gtaggcgctt 4381 tttgcagctc ttcggctgtg cgctggccag acagttatgc acaggccagg cgggttttaa 4441 gagttttaat aagttttaaa gagttttagg cggaaaaatc gccttttttc tcttttatat 4501 cagtcactta catgtgtgac cggttcccaa tgtacggctt tgggttccca atgtacgggt 4561 tccggttccc aatgtacggc tttgggttcc caatgtacgt gctatccaca ggaaagagac 4621 cttttcgacc tttttcccct gctagggcaa tttgccctag catctgctcc gtacattagg 4681 aaccggcgga tgcttcgccc tcgatcaggt tgcggtagcg catgactagg atcgggccag 4741 cctgccccgc ctcctccttc aaatcgtact ccggcaggtc atttgacccg atcagcttgc 4801 gcacggtgaa acagaacttc ttgaactctc cggcgctgcc actgcgttcg tagatcgtct 4861 tgaacaacca tctggcttct gccttgcctg cggcgcggcg tgccaggcgg tagagaaaac 4921 ggccgatgcc gggatcgatc aaaaagtaat cggggtgaac cgtcagcacg tccgggttct 4981 tgccttctgt gatctcgcgg tacatccaat cagctagctc gatctcgatg tactccggcc 5041 gcccggtttc gctctttacg atcttgtagc ggctaatcaa ggcttcaccc tcggataccg 5101 tcaccaggcg gccgttcttg gccttcttcg tacgctgcat ggcaacgtgc gtggtgttta 5161 accgaatgca ggtttctacc aggtcgtctt tctgctttcc gccatcggct cgccggcaga 5221 acttgagtac gtccgcaacg tgtggacgga acacgcggcc gggcttgtct cccttccctt 5281 cccggtatcg gttcatggat tcggttagat gggaaaccgc catcagtacc aggtcgtaat 5341 cccacacact ggccatgccg gccggccctg cggaaacctc tacgtgcccg tctggaagct 5401 cgtagcggat cacctcgcca gctcgtcggt cacgcttcga cagacggaaa acggccacgt 5461 ccatgatgct gcgactatcg cgggtgccca cgtcatagag catcggaacg aaaaaatctg 5521 gttgctcgtc gcccttgggc ggcttcctaa tcgacggcgc accggctgcc ggcggttgcc 5581 gggattcttt gcggattcga tcagcggccg cttgccacga ttcaccgggg cgtgcttctg 5641 cctcgatgcg ttgccgctgg gcggcctgcg cggccttcaa cttctccacc aggtcatcac 5701 ccagcgccgc gccgatttgt accgggccgg atggtttgcg accgtcacgc cgattcctcg 5761 ggcttggggg ttccagtgcc attgcagggc cggcagacaa cccagccgct tacgcctggc 5821 caaccgcccg ttcctccaca catggggcat tccacggcgt cggtgcctgg ttgttcttga 5881 ttttccatgc cgcctccttt agccgctaaa attcatctac tcatttattc atttgctcat 5941 ttactctggt agctgcgcga tgtattcaga tagcagctcg gtaatggtct tgccttggcg 6001 taccgcgtac atcttcagct tggtgtgatc ctccgccggc aactgaaagt tgacccgctt 6061 catggctggc gtgtctgcca ggctggccaa cgttgcagcc ttgctgctgc gtgcgctcgg 6121 acggccggca cttagcgtgt ttgtgctttt gctcattttc tctttacctc attaactcaa 6181 atgagttttg atttaatttc agcggccagc gcctggacct cgcgggcagc gtcgccctcg 6241 ggttctgatt caagaacggt tgtgccggcg gcggcagtgc ctgggtagct cacgcgctgc 6301 gtgatacggg actcaagaat gggcagctcg tacccggcca gcgcctcggc aacctcaccg 6361 ccgatgcgcg tgcctttgat cgcccgcgac acgacaaagg ccgcttgtag ccttccatcc 6421 gtgacctcaa tgcgctgctt aaccagctcc accaggtcgg cggtggccca tatgtcgtaa 6481 gggcttggct gcaccggaat cagcacgaag tcggctgcct tgatcgcgga cacagccaag 6541 tccgccgcct ggggcgctcc gtcgatcact acgaagtcgc gccggccgat ggccttcacg 6601 tcgcggtcaa tcgtcgggcg gtcgatgccg acaacggtta gcggttgatc ttcccgcacg 6661 gccgcccaat cgcgggcact gccctgggga tcggaatcga ctaacagaac atcggccccg 6721 gcgagttgca gggcgcgggc tagatgggtt gcgatggtcg tcttgcctga cccgcctttc 6781 tggttaagta cagcgataac cttcatgcgt tccccttgcg tatttgttta tttactcatc 6841 gcatcatata cgcagcgacc gcatgacgca agctgtttta ctcaaataca catcaccttt 6901 ttagacggcg gcgctcggtt tcttcagcgg ccaagctggc cggccaggcc gccagcttgg 6961 catcagacaa accggccagg atttcatgca gccgcacggt tgagacgtgc gcgggcggct 7021 cgaacacgta cccggccgcg atcatctccg cctcgatctc ttcggtaatg aaaaacggtt 7081 cgtcctggcc gtcctggtgc ggtttcatgc ttgttcctct tggcgttcat tctcggcggc 7141 cgccagggcg tcggcctcgg tcaatgcgtc ctcacggaag gcaccgcgcc gcctggcctc 7201 ggtgggcgtc acttcctcgc tgcgctcaag tgcgcggtac agggtcgagc gatgcacgcc 7261 aagcagtgca gccgcctctt tcacggtgcg gccttcctgg tcgatcagct cgcgggcgtg 7321 cgcgatctgt gccggggtga gggtagggcg ggggccaaac ttcacgcctc gggccttggc 7381 ggcctcgcgc ccgctccggg tgcggtcgat gattagggaa cgctcgaact cggcaatgcc 7441 ggcgaacacg gtcaacacca tgcggccggc cggcgtggtg gtgtcggccc acggctctgc 7501 caggctacgc aggcccgcgc cggcctcctg gatgcgctcg gcaatgtcca gtaggtcgcg 7561 ggtgctgcgg gccaggcggt ctagcctggt cactgtcaca acgtcgccag ggcgtaggtg 7621 gtcaagcatc ctggccagct ccgggcggtc gcgcctggtg ccggtgatct tctcggaaaa 7681 cagcttggtg cagccggccg cgtgcagttc ggcccgttgg ttggtcaagt cctggtcgtc 7741 ggtgctgacg cgggcatagc ccagcaggcc agcggcggcg ctcttgttca tggcgtaatg 7801 tctccggttc tagtcgcaag tattctactt tatgcgacta aaacacgcga caagaaaacg 7861 ccaggaaaag ggcagggcgg cagcctgtcg cgtaacttag gacttgtgcg acatgtcgtt 7921 ttcagaagac ggctgcactg aacgtcagaa gccgactgca ctatagcagc ggaggggttg 7981 gatcaaagta ctttgatccc gaggggaacc ctgtggttgg catgcacata caaatggacg 8041 aacggataaa ccttttcacg cccttttaaa tatccgttat tctaataaac gctcttttct 8101 cttaggttta cccgccaata tatcctgtca aacactgata gtttaaactg aaggcgggaa 8161 acgacaatct gatccaagct caagctaagc ttgggcccga gcggataaca atttcacaca 8221 ggaaacagct atgaccatga ttacgccaag ctatcaactt tgtatagaaa agttgctagt 8281 ctagctcaac agagctttta acccaaattg gtacaataga atacaacttt agatcataat 8341 tctcaaaaga aagagattcc ttagctattc tatctgccac tccatttcct tctcggcttg 8401 tatgcacaag cataaaatcc tcaaacttgc taagtagata ctttatgtct tggataattg 8461 gattgagact tgacaagcat aactttcatg taaccaaaga cacaagttgc tgagaatcca 8521 cctcaaaaat gatcttccta taattgaatc gggataatga cagcacagcc catctaagag 8581 cctccacttc tacttccagc acgcttctta cttttaccac agctcttgca cctaaccata 8641 acaccttccc tgtatgatcg cgaagcaccc accctaagcc acattttaat ccttctgttg 8701 gccatgcccc atcaaagttg cacttaaccc aagattgtgg tggagcttcc catgtttctc 8761 gtctgtcccg acggtgttgt ggttggtgct ttccttacat tctgagcctc tttccttcta 8821 atccactcat ctgcatcttc ttgtgtcctt actaatacct cattggttcc aaattccctc 8881 cctttaagca ccagctcgtt tctgttcttc cacagcctcc caagtatcca agggactaaa 8941 gcctccacat tcttcagatc aggatattct tgtttaagat gttgaactct atggaggttt 9001 gtatgaactg atgatctagg accggataag ttcccttctt catagcgaac ttattcaaag 9061 aatgttttgt gtatcattct tgttacattg ttattaatga aaaaatatta ttggtcattg 9121 gactgaacac gagtgttaaa tatggaccag gccccaaata agatccattg atatatgaat 9181 taaataacaa gaataaatcg agtcaccaaa ccacttgcct tttttaacga gacttgttca 9241 ccaacttgat acaaaagtca ttatcctatg caaatcaata atcatacaaa aatatccaat 9301 aacactaaaa aattaaaaga aatggataat ttcacaatat gttatacgat aaagaagtta 9361 cttttccaag aaattcactg attttataag cccacttgca ttagataaat ggcaaaaaaa 9421 aacaaaaagg aaaagaaata aagcacgaag aattctagaa aatacgaaat acgcttcaat 9481 gcagtgggac ccacggttca attattgcca attttcagct ccaccgtata tttaaaaaat 9541 aaaacgataa tgctaaaaaa atataaatcg taacgatcgt taaatctcaa cggctggatc 9601 ttatgacgac cgttagaaat tgtggttgtc gacgagtcag taataaacgg cgtcaaagtg 9661 gttgcagccg gcacacacga gtcgtgttta tcaactcaaa gcacaaatac ttttcctcaa 9721 cctaaaaata aggcaattag ccaaaaacaa ctttgcgtgt aaacaacgct caatacacgt 9781 gtcattttat tattagctat tgcttcaccg ccttagcttt ctcgtgacct agtcgtcctc 9841 gtcttttctt cttcttcttc tataaaacaa tacccaaaga gctcttcttc ttcacaattc 9901 agatttcaat ttctcaaaat cttaaaaact ttctctcaat tctctctacc gtgatcaagg 9961 taaatttctg tgttccttat tctctcaaaa tcttcgattt tgttttcgtt cgatcccaat 10021 ttcgtatatg ttctttggtt tagattctgt taatcttaga tcgaagacga ttttctgggt 10081 ttgatcgtta gatatcatct taattctcga ttagggtttc atagatatca tccgatttgt 10141 tcaaataatt tgagttttgt cgaataatta ctcttcgatt tgtgatttct atctagatct 10201 ggtgttagtt tctagtttgt gcgatcgaat ttgtcgatta atctgagttt ttctgattaa 10261 caggcaagtt tgtacaaaaa agcaggctta accatggtga gcaagggcga ggagctgttc 10321 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc 10381 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc 10441 accaccggca agctgcccgt gccctggccc accctcgtga ccaccttcgg ctacggcctg 10501 atgtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg 10561 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc 10621 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc 10681 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac 10741 aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc 10801 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc 10861 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gctaccagtc caagctgagc 10921 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg 10981 atcactctcg gcatggacga gctgtacaag tacccagctt tcttgtacaa agtggctacc 11041 atggcgtttg tgaagagtgg ctggttgctg cgacagagta ctattttgaa gcgctggaag 11101 aagaactggt ttgatctgtg gtcggatggt cacctgatct attatgatga ccagactcgg 11161 cagaatatcg aggataaggt ccacatgcca atggactgca tcaacatccg cacggggcag 11221 gaatgtcggg atactcagcc cccggatgga aagtcaaaag actgcatgct ccagattgtt 11281 tgtcgagatg ggaaaacaat tagtctttgt gcagaaagca cagatgattg cttggcctgg 11341 aaatttacac tccaagattc taggacaaac gtcgacggta ccatggcgtt tgtgaagagt 11401 ggctggttgc tgcgacagag tactattttg aagcgctgga agaagaactg gtttgatctg 11461 tggtcggatg gtcacctgat ctattatgat gaccagactc ggcagaatat cgaggataag 11521 gtccacatgc caatggactg catcaacatc cgcacggggc aggaatgtcg ggatactcag 11581 cccccggatg gaaagtcaaa agactgcatg ctccagattg tttgtcgaga tgggaaaaca 11641 attagtcttt gtgcagaaag cacagatgat tgcttggcct ggaaatttac actccaagat 11701 tctaggacaa actagctagc aacttta //