LOCUS MP-linker-1xmCherry_wKO 4142 bp ds-DNA circular 12-MAR-2020 DEFINITION . FEATURES Location/Qualifiers misc_feature 1356..1356 /label="attL2" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 operon 3261..3356 /label="attL1" /ApEinfo_revcolor=#d59687 /ApEinfo_fwdcolor=#d59687 misc_feature complement(3334..3389) /label="R-Primer" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc splicing_signal 3357..3362 /label="KOZAK?" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd misc_feature 3363..3398 /label="MP" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 misc_feature 3390..3444 /label="F-Primer" /ApEinfo_revcolor=#75c6a9 /ApEinfo_fwdcolor=#75c6a9 misc_feature 3399..3422 /label="polyA linker" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 misc_feature 3423..4130 /label="mCherry" /ApEinfo_revcolor=#d59687 /ApEinfo_fwdcolor=#d59687 operon complement(join(4135..4142,1..88)) /label="attL2" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 ORIGIN 1 ttgtacaaag tgggcattat aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc 61 actatcagtc aaaataaaat cattatttgc catccagctg cagctctggc ccgtgtctca 121 aaatctctga tgttacattg cacaagataa aaatatatca tcatgaacaa taaaactgtc 181 tgcttacata aacagtaata caaggggtgt tatgagccat attcaacggg aaacgtcgag 241 gccgcgatta aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa 301 tgtcgggcaa tcaggtgcga caatctatcg cttgtatggg aagcccgatg cgccagagtt 361 gtttctgaaa catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact 421 aaactggctg acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga 481 tgatgcatgg ttactcacca ctgcgatccc cggaaaaaca gcattccagg tattagaaga 541 atatcctgat tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca 601 ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc 661 gcaatcacga atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg 721 ctggcctgtt gaacaagtct ggaaagaaat gcataaactt ttgccattct caccggattc 781 agtcgtcact catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattagg 841 ccgggaagcc gatctcggct tgaacgaatt gttaggtggc ggtacttggg tcgatatcaa 901 agtgcatcac ttcttcccgt atgcccaact ttgtatagag agccactgcg ggatcgtcac 961 cgtaatctgc ttgcacgtag atcacataag caccaagcgc gttggcctca tgcttgagga 1021 gattgatgag cgcggtggca atgccctgcc tccggtgctc gccggagact gcgagatcat 1081 agatatagat ctcactacgc ggctgctcaa acctgggcag aacgtaagcc gcgagagcgc 1141 caacaaccgc ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta cggagcaagt 1201 tcccgaggta atcggagtcc ggctgatgtt gggagtaggt ggctacgtct ccgaactcac 1261 gaccgaaaag atcaagagca gcccgcatgg atttgacttg gtcagggccg agcctacatg 1321 tgcgaatgat gcccatactt gagccaccta actttgtttt agggcgactg ccctgctgcg 1381 taacatcgtt gctgctgcgt aacatcgttg ctgctccata acatcaaaca tcgacccacg 1441 gcgtaacgcg cttgctgctt ggatgcccga ggcatagact gtacaaaaaa acagtcataa 1501 caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac 1561 cagttgcgtg agcgcatacg ctacttgcat tacagtttac gaaccgaaca ggcttatgtc 1621 aactgggttc gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac cttgggcagc 1681 agcgaagtcg aggcatttct gtcctggctg gcgaacgagc gcaaggtttc ggtctccacg 1741 catcgtcagg cattggcggc cttgctgttc ttctacggca aggtgctgtg cacggatctg 1801 ccctggcttc aggagatcgg aagacctcgg ccgtcgcggc gcttgccggt ggtgctgacc 1861 ccggatgaag tggttcgcat cctcggtttt ctggaaggcg agcatcgttt gttcgcccag 1921 gactctagct atagttctag tggttggcta ctaataggtt gtattgatgt tggacgagtc 1981 ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg tgagttttct 2041 ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga tatgaataaa 2101 ttgcagtttc atttgatgct cgatgagttt ttctaatcag aattggttaa ttggttgtaa 2161 cactggcaga gcattacgct gacttgacgg gacggcgcaa gctcatgacc aaaatccctt 2221 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt 2281 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag 2341 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca 2401 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca 2461 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg 2521 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg 2581 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct 2641 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga 2701 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc 2761 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg 2821 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg 2881 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt 2941 tatcccctga ttctgtggat aaccgtatta ccgctagcca ggaagagttt gtagaaacgc 3001 aaaaaggcca tccgtcagga tggccttctg cttagtttga tgcctggcag tttatggcgg 3061 gcgtcctgcc cgccaccctc cgggccgttg cttcacaacg ttcaaatccg ctcccggcgg 3121 atttgtccta ctcaggagag cgttcaccga caaacaacag ataaaacgaa aggcccagtc 3181 ttccgactga gcctttcgtt ttatttgatg cctggcagtt ccctactctc gcgttaacgc 3241 tagcatggat ctcgggcccc aaataatgat tttattttga ctgatagtga cctgttcgtt 3301 gcaacaaatt gatgagcaat gcttttttat aatgccaagt ttgtacaaaa aagcaggcta 3361 ccatgggctt actctgcagt agaagtcgac atcatactcc tttgcctgct gccgctgccg 3421 ctatggtgag caagggcgag gaggataaca tggccatcat caaggagttc atgcgcttca 3481 aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg 3541 gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt ggccccctgc 3601 ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc 3661 accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc aagtgggagc 3721 gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg 3781 acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc gacggccccg 3841 taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac cccgaggacg 3901 gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc cactacgacg 3961 ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc gcctacaacg 4021 tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg 4081 aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag tacccagctt 4141 tc //