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Yvon JAILLAIS
yvon.jaillais
(33)4 72 72 86 09
DR CNRS
Publications
Isabelle FOBIS-LOISY
Isabelle.Fobis-Loisy
(33) 4 72 72 89 85
CRCE CNRS
Publications
Christine MIEGE
Christine.Miege
(33) 4 72 72 86 09
MC ENS
Vincent BAYLE
vincent.bayle11
(33) 4 72 72 86 10
IR CNRS
Frédérique ROZIER
Frederique.Rozier
(33) 4 72 72 86 05
IE CNRS
Camila GOLDY
camila.goldy
Postdoc (EMBO fellowship)
Clara IGISCH
clara.igisch
Ph.D student
Ingrid REVEL
Engineer (ANR funded)
Lucas COURGEONS
AGPR ENS de Lyon
Ludivine BROCARD
ludivine.brocard
Assistant engineer (ANR funded)
Nelson SERRE
nelson.serre
Postdoc
Patricia SCHOLZ
patricia.scholz
Postdoc (EMBO fellowship)
Samantha MOULIN
IE CNRS (ANR funded)
Vedrana MARKOVIC
vedrana.markovic
Postdoc (EMBO fellowship)
Yoko Hasegawa
Yoko.Hasegawa
JSPS long-term fellow
2025
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Preprints, Working Papers, ...
2024
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2023
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2022
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2021
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2020
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2019
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2018
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2017
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2016
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2015
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2014
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2013
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2012
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2010
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2009
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2008
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2007
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2006
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2005
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1995
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The main focus of the Cell Signaling group is to unravel the mechanisms behind cell-cell communication in plants.
Plant growth and development are regulated by interactions between the environment and endogenous developmental programs. The “Cell Signaling” lab is interested in identifying the molecular and cellular mechanisms behind signal transduction and cell-cell communication in plants. We use Arabidopsis thaliana, an ideal organism for integrative biology, genetics and molecular investigations of signaling networks.
The “Cell Signaling” lab is composed of two groups that study cell signaling with an emphasis on receptor kinases and intracellular trafficking:
– Pollen-pistil interaction
– Lipid signaling in plant cell division and organogenesis
– Lipid signaling in development and environmental interactions
– Ressources: plasmid maps and transgenic lines
Leader : Isabelle Fobis-Loisy
Plant reproduction is responsible for a large part of our food supply, with seed production essential to crop yields and therefore food security. In the Brassicaceae family, including important crops and the model plant Arabidopsis thaliana, reproduction begins with an intricate cell-to-cell communication initiated by the contact between the pollen grain (the carrier for the male sperm cells) and the stigma epidermis (the receptive platform of the reproductive female organ). Once landed at the stigma surface, the pollen grain forms a tube responsible for transporting sperm cells to the ovules deeply embedded within the female organ. Through a multidisciplinary approach, we aim to unravel the molecular, cellular and mechanical mechanisms underlying the early communication events between pollen and stigma. Our project is structured around two main themes:
– We developed a live imaging system with a fast-scanning confocal microscope to monitor Arabidopsis pollen behaviour at the stigma surface and provided a thorough analysis of the dynamics of the cellular events following pollen perception (Rozier et al., 2020; DOI:10.1093/jxb/eraa008; Riglet et al., 2024; DOI: 10.1093/jxb/erae308). This semi-in vivo system provides a particularly well-suited system to test putative components involved in the acceptance or rejection of pollen grains.
– An intriguing aspect of the pollen tube growth in Arabidopsis thaliana is its ability to penetrate the cell wall of the stigmatic cell and subsequently navigate while being constrained within this rigid layer. We have recently underscored the significant role of mechanical properties of the stigmatic cell wall in influencing directionality of the pollen tube growth (Riglet et al., 2020; DOI:10.7554/eLife.57282). As accessing most mechanical properties of the stigma epidermis experimentally proves challenging, we used mathematical modelling (in collaboration with Christophe Godin at the RDP and Karin John from the university of Grenoble) to investigate the mechanisms underlying early pollen tube guidance. We identified a potential guidance mechanism driven by the geometry of the stigmatic cells and the elastic properties of their cell walls (under review in PLOS Computational Biology). Our major challenge now is to establish a link between our model predictions and the spatial organisation of the stigmatic cell wall, which is still largely unknown. To improve our knowledge of this unique cell layer, we plan to test straightforward approaches such as Immunolabeling couple with electron microscopy, multicolor 3D dSTORM nanoscopy, or expansion microscopy.
Leader : Marie-Cecile Caillaud
How plant cells positioned their division planes during morphogenesis ?
During the life cycle of any multicellular organism, cell division contributes to the production of specialized cells necessary for forming tissues and performing particular functions. Therefore, the mechanisms of cell division must be tightly regulated because dysfunctions in their control can lead to tumour formation or developmental defects. A crucial feature of cell division is how cells position their division plane. This is even more important for organisms with walls because their cells are embedded and cannot move.
A marker located at the equatorial periphery of the cell maintains the memory of the cell division plan throughout plant cell division. Although the importance of division plane orientation for tissue topology and morphogenesis is undisputed, research on division plane positioning primarily focuses on cell-autonomous signals.
It was hypothesized that mechanical stresses at the tissue scale could influence the orientation of the division plane. Yet it is unclear how these cues, let alone local tissue topology, are integrated into division plane positioning. We currently wish to build an integrated vision of the cell division process in a multicellular context and consider cell division’s orientation as a non-cellular autonomous mechanism relying on signals coming from the local environment.
Using a combined approach of cell biology, molecular genetics and multi-scale mechanical modelling, we will study how plant cells direct their division by integrating biophysical and biochemical signals from surrounding tissues.
We will address the following questions in particular:
Leader : Yvon Jaillais
How individual cells communicate with each other and with the environment to shape plant architecture?
Plants are sessile organisms and therefore must constantly adapt their growth and architecture to an ever-changing environment. It is the delicate balance between developmental and environmental signals that shapes the architecture of the plants. Individual cells constantly integrate those cues into cellular output, that gives rise to new organs. Cells are equipped with receptor molecules that allow communication with other cells in the organism and detection of changes in their surrounding environment, such as changes in light quality, nutrient availability, attack by pathogens...
The plasma membrane plays a critical role in regulating exchanges between the intra- and extracellular milieu and in controlling cell-to-cell communication. The aim of our group is to understand what makes the plasma membrane unique with regard to other membrane compartments and as such competent for signaling. We want to understand how signaling domains are being defined at the plant plasma membrane, both at the level of the cell (e.g., polar domains) but also at the nanometer scale (e.g., membrane nanodomains). We also plan to address how these signaling domains evolve during the course of signaling and what are their functional roles during cell-cell communication.
Anionic phospholipids (e.g. phosphoinositides, PIPs) are minor lipids in membranes but they have a huge impact on cell signaling and organelle identity. They not only recruit proteins to specific cellular compartments, but they also deeply impact membrane biophysics properties. Furthermore, their production is tightly and dynamically regulated during development and interaction with the environment.
We now want to address :
– What are the anionic lipids involved in plasma membrane organization and how?
– What are the functions of anionic lipids and their interacting proteins in hormone signaling? (e.g. brassinosteroid and auxin signaling)
– How are anionic lipids orchestrating membrane trafficking and cell polarity?
– How is anionic lipids homeostasis regulated by changes in the environment and contribute to plant adaptation?
Please find all the information here.
– Permanent staff
Christine Miège - MCF, ENS Lyon
Frédérique Rozier - IE - CNRS
Isabelle Fobis-Loisy - CR, CNRS
Marie-Cécile Caillaud - DR, CNRS
Vincent Bayle - IR, CNRS
Yvon Jaillais - DR (Groupe leader), CNRS
– Post-docs
Camila Goldy (EMBO long-term fellow)
Vedrana MARKOVIC (EMBO long-term fellow)
Nelson SERRE (ERC funded)
Yoko HASEGAWA (JSPS fellow)
Patricia SCHOLTZ (EMBO long-term fellow)
Jonathas PEREIRA GRACAS (ANR funded)
Lucas Courgeons (PRAG ENS of Lyon)
Hortense Moreux (ANR-funded shared postdoc with Emmanuelle Bayer’s team)
– PhD student
Clara IGISCH - Doctorante - (ERC funded)
– technical staff
Samantha Moulin (engineer, ANR funded)
Timothée Cavanna (assistant engineer, ERC funded)
Ingrid REVEL (engineer, ANR funded)
PhD students
Gwennogan Dubois (2019-2023)
Alexis Lebecq (2019-2022)
Lise Noack (2017-2021)
Mehdi Doumane (2017-2020)
Lucie Riglet (2015-2018)
Matthieu Platre (2014-2017)
Mathilde Simon (2012-2015)
Jonathan Schnabel (2010-2013)
Postdoc:
Chloé Beziat (2019-2024)
Laia Armengot (2016-2020)
Thomas Stanislas (2015-2018 shared postdoc with Olivier Hamant’s team)
Chie Kodera (2015-2018)
Mar Marques-bueno (2013-2016)
Eleonore Durand (2012-2014)
Enric Zelazny (2011-2013)
Thomas Durand (2011-2012)
Technical staff
Aurélie Fangain (2017-2022)
Elsa Gascon (2019-2020)
Mengying Liu (2019-2020)
Joseph Bareil (2017-2019)
Internship IUT, L3, M1, M2:
– Samantha Moulin (2024 - M2 Univ Grenoble-Milan)
– Anastasia HELD (2024 - stage de 3°)
– Ingrid Revel (2024 - M2 UCBL)
– Oceane Porcheron (2023 - BTS Biotechnologie au Lycée Simone Weil au Puy en Velay)
– Lise Plessis (2023 - L3 SupBiotech Lyon)
– Elio Langlois (2023 - L3 UCBL)
– Ingrid Revel (2023 - M1 UCBL)
– Lylou RAVIER (2022 - M1 UCBL)
– Marie Foyot (2022 - M2 Université de Bourgogne, Dijon)
– Alisee Pete-Bonneton (2021 - M2 Univ Lyon)
– Elsa Gascon (2020 - M2 INSA Lyon)
– Guerin Amandine (2020 - M2 from ENSA Rennes)
– Alice Boussaroque (2020 -L3 ENS de Lyon)
– Alexis Lebecq (2019 - M2 UNSA Univ Nice)
– Amelie Bauer (2019 - M1 génétique et biologie cellulaire, Univ Lyon1)
– Lucas Courgeon (2018 - L3 bioscience ENS Lyon)
– Paul Louis Lopez-Marnet (M2 - Univ Bordeaux)
– Alvaro Garcia (2017 - visiting PhD student, Malaga, Spain> Miguel Botella lab)
– Hanh Nguyen (2017 - M1 Univ Lyon1 - Master Biologie Vegetale)
– Clément Saccaro (2016 - IUT Univ Lyon 1)
– Dasha Novikova (2015 - master exchange program)
– Allan Bernard (2015 - L3 bioscience ENS Lyon)
– Lucie Riglet (2014 - M2 PURPAN Toulouse)
– Lea Gini (2014, M2, Strabsourg university)
– Lise Noack (2014 - L3 bioscience ENS Lyon)
– Gena Salem (2014 - L3 Univ Lyon 1)
– Eline Guillon (2014 - 2nd year IUT Genie biologique, option Agronomie, Aurillac)
– Ghislaine Laroche (2013 - 3rd year student Isara Lyon)
– Amelie Achard (2013, M2, ISARA Lyon)
– Amandine Basset (2013 - L3 bioscience ENS Lyon - 2014 - M1 Bioscience ENS Lyon)
– Chloé Cardot (2012 - M1 microbiologie écoscience option phytoressource Univ Lyon1)
The PIPline
The PIPline is a multicolor and multi affinity marker set that highlights membrane-associated phosphoinositides (PIPs) in Arabidopsis.
Seeds can be ordered as individual stocks or as a collection at ABCR (ABRC stock set #CS2105604) or NASC (NASC stock set # N21056604)
All the (…)
