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Séminaire RDP de Chloe ZUBIETA

Monday 29 September Chloe ZUBIETA from Laboratoire de Physiologie Cellulaire & Végétale (LPCV), CEA Grenoble will give a seminar on :

Probing the Biophysical Basis for MADS Transcription Factor Function - structural and biochemical studies of SEPALLATA3

The seminar will be held at "Salle des thèses" at 11h.

Chloe has long experience in protein structure and the past few years she focussed her work on structure-function on MADS TFs that play important roles in floral organ initiation and development.

Summary : The MADS family of transcription factors (TFs) plays crucial roles in many aspects of plant reproduction, in particular flowering and floral organ development. Our team uses a combination of structural and biophysical techniques to study these TFs in order to understand their function at the atomic and molecular level. Our current research focuses on SEPALLATA3 (SEP3), a MADS TF which plays a role in the development of all floral organs via its ability to form specific tetrameric complexes with other MADS TFs. We have recently structurally characterized the oligomerization domain of SEP3 and use this as a model to understand and predict the protein-protein interaction patterns of the larger family of MADS TFs. With this biophysical foundation, the complex processes of floral organ development orchestrated by the MADS transcription factor family can be tied to molecular level events.

- Totir, M., Echols, N., Nanao, M., Gee, C.L., Moskaleva, A., Gradia, S., Iavarone, A.T., Berger, J.M., May, A.P., Zubieta, C., and Alber, T. (2012). Macro-to-micro structural proteomics : native source proteins for high-throughput crystallization. PLoS One 7(2):e32498

- Westfall, C.S., Zubieta, C., Herrmann, J., Kapp, U., Nanao, M.H., and Jez, J.M. (2012). Structural basis for prereceptor modulation of plant hormones by GH3 proteins. Science 336, 1708-1711.

- Acajjaoui, S., and Zubieta, C. (2013). Crystallization studies of the keratin-like domain from Arabidopsis thaliana SEPALLATA 3. Acta Crystallogr Sect F Struct Biol Cryst Commun 69, 997-1000.

- Costa, L., Rodrigues, M.S., Newman, E., Zubieta, C., Chevrier, J., and Comin, F. (2013). Imaging material properties of biological samples with a force feedback microscope. J Mol Recognit 26, 689-693.

- Puranik, S., Acajjaoui, S., Conn, S., Costa, L., Conn, V., Vial, A., Marcellin, R., Melzer, R., Brown, E., Hart, D., Theissen, G., Silva, C.S., Parcy, F., Dumas, R., Nanao, M., and Zubieta, C. (2014). Structural Basis for the Oligomerization of the MADS Domain Transcription Factor SEPALLATA3 in Arabidopsis. Plant Cell (in press).

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