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Seed Development

Personnel du RDP

Gwyneth INGRAM

gwyneth.ingram
(33) 4 26 23 39 78
DR2 CNRS

Peter ROGOWSKY

Peter.Rogowsky
(33) 4 72 72 86 07
DR1 INRA

Thomas WIDIEZ

thomas.widiez
(33) 4 72 72 86 08
CR1 INRA

Nathalie DEPEGE-FARGEIX

Nathalie.Depege
(33) 4 72 72 89 85
MC UCBL

Audrey CREFF

audrey.creff
(33) 4 72 72 89 85
IE CNRS

Jérome LAPLAIGE

jerome.laplaige
04 72 72 86 07
CDD IE INRA

Ghislaine GENDROT

ghislaine.gendrot
(33) 4 72 72 86 07
AI INRA

Christelle RICHARD

christelle.richard
(33) 4 72 72 85 91
CDD AI INRA

Edwige DELAHAYE

edwige.delahaye1
(33) 4 72 72 85 91
CDD AI INRA

Sophy CHAMOT

sophy.chamot
(33) 4 72 72 86 14
TCE CNRS

Anne Charlotte MARSOLLIER

AGPR

Douglas PYOTT

Postdoc

Laurine GILLES

laurine.gilles
04 72 72 39 59
Thèse

Nicolas DOLL

nicolas.doll
04 72 72 86 10
Thèse

Jeanne Loue MANIFEL

jeanne.loue-manifel
Thèse



2018


Ingram G, North H, Lepiniec L. "Seeds as perfect factories for developing sustainable agriculture". Plant Reproduction https://doi.org/10.1007/s00497-018-0340-7 (2018)
PMID: 29934741

Marsollier AC, Ingram G. "Getting physical: invasive growth events during plant development.". Curr Opin Plant Biol. (2018)
PMID: 29981931

Lang D, Ullrich KK, Murat F, Fuchs J, Jenkins J, Haas FB, Piednoel M, Gundlach H, Van Bel M, Vives Cobo, Morata J, Symeonidi A, Hiss M, Meyberg R, Muchero W, Kamisugi Y, Saleh O, Blanc G, Decker EL, van Gessel N, Grimwood J, Hayes R, Graham SW, Gunter LE, McDaniel S, Hoernstein SNW, Larsson A, Li FW, Phillips J, Ranjan P, Rokshar DS, Rothfels CJ, Schneider L, Shu S, Stevenson DW, Thümmler F, Tillich M, Villarreal JCA, Widiez T, Wong GKS, Wymore A, Zhang Y, Zimmer AD, Quatrano RS, Mayer KFX, Goodstein D, Casacuberta JM, Vandepoele K, Reski R, Cuming AC, Tuskan J, Maumus F, Salse J, Schmutz J, Rensing SA. "The P. patens chromosome-scale assembly reveals moss genome structure and evolution". Plant Journal (2018)
PMID: 29237241

Darracq A, Vitte C, Nicolas S, Duarte J, Pichon JP, Mary-Huard T, Chevalier C, Bérard A, Le Paslier MC, Rogowsky P, Charcosset A, Joets J. "Sequence analysis of European maize inbred line F2 provides new insights into molecular and chromosomal characteristics of presence/absence variants". BMC Genomics. (2018)
PMID: 29402214

Eriksson D, Harwood W, Hofvander P, Jones H, Rogowsky P, Stöger E, Visser RGF. "A Welcome Proposal to Amend the GMO Legislation of the EU". Trends in Biotechnology in press Elsevier (2018)
PMID: 29807731

Malivert A, Hamant O, Ingram G. "The contribution of mechanosensing to epidermal cell fate specification.". Curr Opin Genet Dev. (2018)
PMID: 30006098

Borrelli VMG, Brambilla V, Rogowsky P, Marocco A, Lanubile A. "The Enhancement of Plant Disease Resistance Using CRISPR/Cas9 Technology". Frontiers in Plant Science 9:1245 (2018)
PMID: 30197654
PDF

2017


"Ingram GC. Dying to live: cell elimination as...". (2017)
Ingram GC.
Dying to live: cell elimination as a developmental strategy in angiosperm seeds.
J Exp Bot. [PMID: 27702990]

"Meyer HM, Teles J, Formosa-Jordan P, Refahi...". (2017)
Meyer HM, Teles J, Formosa-Jordan P, Refahi Y, San-Bento R, Ingram G, Jönsson H, Locke JC, Roeder AH.
Fluctuations of the transcription factor ATML1 generate the pattern of giant cells in the Arabidopsis sepal
Elife. 2017 [PMID: 28145865]

"Doll MN, Depège-Fargeix N, Rogowsky PM, Widiez...". (2017)
Doll MN, Depège-Fargeix N, Rogowsky PM, Widiez T.
Signaling in early maize kernel development
Molecular Plant. 2017 [PMID: 28267956]

"Gilles LM, Khaled A, Laffaire JB, Chaignon S,...". (2017)
Gilles LM, Khaled A, Laffaire JB, Chaignon S, Gendrot G, Laplaige J, Bergès H, Beydon G, Bayle V, Barret P, Comadran J, Martinant JP, Rogowsky PM, Widiez T.
Loss of pollen-specific phospholipase NOT LIKE DAD triggers gynogenesis in maize
EMBO J. 2017 [PMID: 28228439]
Featured in EMBO J; INRA; CNRS; ENS de Lyon

"Amanda D, Doblin MS, Galletti R, Bacic A,...". (2017)
Amanda D, Doblin MS, Galletti R, Bacic A, Ingram GC, Johnson KL
Regulation of Cell Wall Genes in Response to DEFECTIVE KERNEL1 (DEK1)-Induced Cell Wall Changes.
Plant Signal Behav [PMID: 28692330]

"Moussu SA, Doll NM, Chamot S, Brocard L,...". (2017)
Moussu SA, Doll NM, Chamot S, Brocard L, Creff A, Fourquin C, Widiez T, Nimchuk ZL, Ingram GC.
ZHOUPI and KERBEROS Mediate Embryo/Endosperm Separation by Promoting the Formation of an Extra-Cuticular Sheath at the Embryo Eurface.
Plant Cell [PMID: 28696222]

"Ingram G, Nawrath C. The roles of the cuticle...". (2017)
Ingram G, Nawrath C. The roles of the cuticle in plant development: organ adhesions and beyond. J Exp Bot. [PMID: 28992283]

"Tran D, Galletti R, Neumann ED, Dubois A,...". (2017)
Tran D, Galletti R, Neumann ED, Dubois A, Sharif-Naeini R, Geitmann A, Frachisse JM, Hamant O, Ingram GC A mechanosensitive Ca2+ channel activity is dependent on the developmental regulator DEK1. Nat Commun. [PMID: 29044106]

"Gilles LM, Martinant JP, Rogowsky PM, Widiez...". (2017)
Gilles LM, Martinant JP, Rogowsky PM, Widiez T. Haploid induction in plants. Current Biology [PMID: 29065285]

"Widiez, T., Ingram, G. C. & Gutierrez-Marcos,...". (2017)
Widiez, T., Ingram, G. C. & Gutierrez-Marcos, J. Embryo-Endosperm-Sporophyte Interaction in Maize Seeds. in Maize Kernel Development 95–107 (Brian A. Larkins, 2017). [Link to the book]

"Amanda D, Doblin MS, MacMillan CP, Galletti...". (2017)
Amanda D, Doblin MS, MacMillan CP, Galletti R, Golz JF, Bacic A, Ingram GC, Johnson KL. Arabidopsis DEFECTIVE KERNEL1 regulates cell wall composition and axial growth in the inflorescence stem. Plant Direct [Link]

"Youssef D, Nihou A, Partier A, Tassy C, Paul...". (2017)
Youssef D, Nihou A, Partier A, Tassy C, Paul W, Rogowsky PM, Beckert M and Barret P. Induction of targeted deletions in transgenic bread wheat (Triticum aestivum L.) using customised meganuclease.
Plant Mol Biol Rep [Link to]

2016


"Delude C, Moussu S, Joubès J, Ingram G and...". (2016)
Delude C, Moussu S, Joubès J, Ingram G and Domergue F.
Plant surface lipids and epidermis development. Lipids in Plant and Algae Development. Subcell Biochem. [PMID: 27023240]

"Ingram G, Fujiwara T. Special Focus Issue on...". (2016)
Ingram G, Fujiwara T.
Special Focus Issue on Plant Responses to the Environment.
Plant Cell Physiol. [PMID: 2016 27091852]

"Fourquin C, Beauzamy L, Chamot S, Creff A,...". (2016)
Fourquin C, Beauzamy L, Chamot S, Creff A, Goodrich J, Boudaoud A, Ingram G.
Mechanical stress mediated by both endosperm softening and embryo growth underlies endosperm elimination in Arabidopsis seeds.
Development. [PMID: 27287798]

"Beauzamy L, Fourquin C, Dubrulle N, Boursiac...". (2016)
Beauzamy L, Fourquin C, Dubrulle N, Boursiac Y, Boudaoud A, Ingram G.
Endosperm turgor pressure decreases during early Arabidopsis seed development.
Development. [PMID: 27287811]

"Galletti R, Verger S, Hamant O, and Ingram G....". (2016)
Galletti R, Verger S, Hamant O, and Ingram G.
Developing a ‘thick skin’ : a paradoxical role for mechanical tension in maintaining epidermis integrity?
Development. [PMID: 27624830]

"Sucher J, Boni R, Yang P, Rogowsky P, Kumlehn...". (2016)
Sucher J, Boni R, Yang P, Rogowsky P, Kumlehn J, Krattinger SG, Keller B.
The durable wheat disease resistance gene Lr34 confers common rust and northern corn leaf blight resistance in maize. Plant Biotech J. [PMID: 27734576]

"Amanda D, Doblin MS, Galletti R, Bacic A,...". (2016)
Amanda D, Doblin MS, Galletti R, Bacic A, Ingram GC, Johnson KL.
DEFECTIVE KERNEL1 (DEK1) regulates cell walls in the leaf epidermis Plant Physiol. 2016 [PMID: 27756823]

2015


"Creff A, Brocard L, Ingram G. A mechanically...". (2015)
Creff A, Brocard L, Ingram G.
A mechanically sensitive cell layer regulates the physical properties of the Arabidopsis seed coat.
Nat Commun. 2015 [PMID: 25702924]

"Galletti R, Johnson KL, Scofield S, San-Bento...". (2015)
Galletti R, Johnson KL, Scofield S, San-Bento R, Watt AM, Murray JA, Ingram GC.
DEFECTIVE KERNEL 1 promotes and maintains plant epidermal differentiation. Development. 2015 [PMID: 25953348]

"McLaughlin JE, Bin-Umer MA, Widiez T, Finn D,...". (2015)
McLaughlin JE, Bin-Umer MA, Widiez T, Finn D, McCormick S, Tumer NE.
A Lipid Transfer Protein Increases the Glutathione Content and Enhances Arabidopsis Resistance to a Trichothecene Mycotoxin. [PMID: 26057253]

"Grimault A, Gendrot G, Chaignon S, Gilard F,...". (2015)
Grimault A, Gendrot G, Chaignon S, Gilard F, Tcherkez G, Thévenine J, Dubreucq B, Depège-Fargeix N, Rogowsky PM.
Role of B3 domain transcription factors of the AFL family in maize kernel filling. Plant Science. 2015 [PMID: 26025525]

"Grimault A, Gendrot G, Chaignon S, Gilard F,...". (2015)
Grimault A, Gendrot G, Chaignon S, Gilard F, Tcherkez G, Thévenine J, Dubreucq B, Depège-Fargeix N, Rogowsky PM.
Role of B3 domain transcription factors of the AFL family in maize kernel filling. Plant Science. 2015 [PMID: 26025525]

"La Rocca N, Manzotti PS, Cavaiuolo M,...". (2015)
La Rocca N, Manzotti PS, Cavaiuolo M, Barbante A, Dalla Vecchia F, Gabotti D, Gendrot G, Horner DS, Krstajic J, Persico M, Rascio N, Rogowsky P, Scarafoni A, Consonni G.
The maize fused leaves1 (fdl1) gene controls organ separation in the embryo and seedling shoot and promotes coleoptile opening.
J Exp Bot. 2015 [PMID: 26093144]

"Ingram G and Gutierrez-Marcos J. Peptide...". (2015)
Ingram G and Gutierrez-Marcos J.
Peptide signalling during angiosperm seed development.
J Exp Bot. 2015 [PMID: 26195729]

"Galletti R and Ingram GC. Communication is...". (2015)
Galletti R and Ingram GC.
Communication is key: reducing DEK1 activity reveals a link between cell-cell contacts and epidermal cell differentiation status.
Communicative & Integrative Biology, 2015 [PMID: 27064205]

"Grimault A, Gendrot G, Chamot S, Widiez T,...". (2015)
Grimault A, Gendrot G, Chamot S, Widiez T, Rabillé H, Gérentes M-F, Creff A, Thévenin J, Dubreucq B, Ingram GC, Rogowsky PM, and Depège-Fargeix, N.
ZmZHOUPI, an endosperm-specific bHLH transcription factor involved in maize seed development.
Plant J. 2015 [PMID: 26361885]

"Sosso D, Luo D, Li QB, Sasse J, Yang J,...". (2015)
Sosso D, Luo D, Li QB, Sasse J, Yang J, Gendrot G, Suzuki M, Koch KE, McCarty DR, Chourey PS, Rogowsky PM, Ross-Ibarra J, Yang B, Frommer WB.
Seed filling in domesticated maize and rice depends on SWEET-mediated hexose transport.
Nat Genet. 2015 [PMID: 26523777]

"Rousseau D*, Widiez T*, Di Tommaso S, Rositi...". (2015)
Rousseau D*, Widiez T*, Di Tommaso S, Rositi H, Adrien J, Maire E, Langer M, Olivier C, Peyrin F, Rogowsky P.
Fast virtual histology using X-ray in-line phase tomography: application to the 3D anatomy of maize developing seeds.
Plant Methods. 2015 [PMID:26688690]

2014


"San-Bento R, Farcot E, Galletti R, Creff A,...". (2014)
San-Bento R, Farcot E, Galletti R, Creff A, Ingram G.
Epidermal identity is maintained by cell-cell communication via a universally active feedback-loop in Arabidopsis thaliana. Plant Journal [PMID: 24147836]

"Denay G, Creff A, Moussu S, Wagnon P, Thévenin...". (2014)
Denay G, Creff A, Moussu S, Wagnon P, Thévenin J, Gérentes MF, Chambrier P, Dubreucq B, Ingram G.
Endosperm breakdown in Arabidopsis requires heterodimers of the basic helix-loop-helix proteins ZHOUPI and INDUCER OF CBP EXPRESSION Development. 2014 [PMID: 24553285]

"Smolarkiewicz M, Skrzypczak T, Michalak M,...". (2014)
Smolarkiewicz M, Skrzypczak T, Michalak M, Leśniewicz K, Walker JR, Ingram G, Wojtaszek P.
Gamma-secretase subunits associate in intracellular membrane compartments in Arabidopsis thaliana. J Exp Bot. 2014 [PMID:24723404]

2013


"Waters, A., Creff, A., Goodrich, J., and...". (2013)
Waters, A., Creff, A., Goodrich, J., and Ingram, G. (2013). "What we’ve got here is failure to communicate": Zou mutants and endosperm cell death in seed development. Plant Signal Behav.

"Xing, Q., Creff, A., Waters, A., Tanaka, H.,...". (2013)
Xing, Q., Creff, A., Waters, A., Tanaka, H., Goodrich, J., and Ingram, G.C. (2013). ZHOUPI controls embryonic cuticle formation via a signalling pathway involving the subtilisin protease ABNORMAL LEAF-SHAPE1 and the receptor kinases GASSHO1 and GASSHO2. Development 140, 770-779.

"Moussu S, San-Bento R, Galletti R, Creff A,...". (2013)
Moussu S, San-Bento R, Galletti R, Creff A, Farcot E, Ingram G.
Embryonic cuticle establishment: The great (apoplastic) divide. Plant Signal Behav. 2014 [PMID: 24398513]




The team aims to decipher mechanisms that govern seed development in the model plant Arabidopsis and the crop plant maize.


Current Projects

- Communication between seed compartments
- Mechanics of seed development
- Haploid embryo induction
- Biotechnology

Communication between seed compartments

Leaders: Gwyneth INGRAM (Arabidopsis) / Nathalie DEPEGE-FARGEIX & Thomas WIDIEZ (maize)

Seeds are complex biological systems composed of three compartments: the maternal tissues (represented in green on the picture here opposite), the embryo (in red), and its nourishing tissue the endosperm (in blue). In addition to actively importing and exporting nutrients, these tissues also undergo profound and tightly synchronized developmental transitions during seed development. This emphasizes the need to establish communication between these three different tissues in order to co-ordinate their developmental programs throughout the seed development.
The main questions tackled in the two plant models (Arabidopsis and maize) are:
- What are the signaling mechanisms and frameworks operating between the three main seed compartments?
- How is the structure/composition of the apoplast at compartment interfaces controlled, and how does interface structure/composition influence inter-compartmental communication?


Mechanics of seed development

Leaders: Gwyneth INGRAM & Benoit LANDREIN

Using the seed of Arabidopsis as a model system, we are studying how cell and tissue mechanics control the emergence of a specific shape during development. We have shown that the growth of the seed is driven by the turgor of the expanding endosperm (Beauzamy et al. 2016, Development) and that a specific layer of the seed coat (the adaxial epidermis of the outer integument) is able to respond to the tension induced by the growing endosperm by stiffening its inner cell wall thus revealing the existence of mechanical signaling within the developing seed (Creff et al. 2015, Nature Communications). Using a combination of experimental and modelling approaches in collaboration with other teams in the laboratory, we are studying further how the mechanical properties of the endosperm and the testa are regulated and influence seed morphogenesis.


Haploid embryo induction

Leader: Thomas WIDIEZ

Peculiar maize lines (called “haploid inducer lines”) present a deviation from the classical plant double fertilization, leading to the production of atypical seeds containing haploid embryos (containing only the maternal genome, and a normal endosperm. Our pioneering work (Gilles et al. 2017, EMBO Journal) identified the underlying mutation in a phospholipase that we named NOT LIKE DAD (NLD) because haploid embryos lack a paternal contribution. This project aims at solving the mystery of how the pollen (male) expressed gene NLD induces maternal (female) haploid embryos. In addition to tackling fundamental questions in plant reproduction, this project is also strongly connected to plant breeding, because maize haploid inducer lines have become key tools for seed companies as part of in planta doubled haploid technologies, which allow rapid production of perfectly homozygous maize plants (see our mini-review:Gilles et al. 2017, Current Biology).


Biotechnology

Leader: Peter ROGOWSKY

Biotechnological research in the team is centered around the maize transformation platform founded in 2008. The vocation of the platform is to produce transgenic maize plants for fundamental research and all plants produced are cultured exclusively in confined environments. The biological questions concern on one hand maize reproduction and kernel development in liaison with the SeedDev team, and on the other flowering time, tolerance to water deficit and nitrogen use efficiency in collaboration with national and international partners. In parallel, technological developments are carried out to improve maize transformation, for example by increasing the transformation rate, by shortening the duration of the transformation process or by adapting it to genome editing by CRISPR/Cas9.


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