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Floral Morphogenesis

Personnel du RDP


(33) 4 72 72 89 84


(33) 4 26 23 39 79

Philippe VERGNE

(33) 4 26 23 39 79


(33)4 72 72 86 11

Jérémy JUST

(33)4 72 72 86 11

Veronique BOLTZ

(33) 4 72 72 86 08


(33) 4 72 72 86 06

Priscilla VILLAND

(33) 4 72 72 86 12


(33) 4 72 72 39 59




Doll NM, Gilles LM, Gérentes MF, Richard C, Just J, Fierlej Y, Borrelli VMG, Gendrot G, Ingram GC, Rogowsky PM, Widiez T. "Single and multiple gene knockouts by CRISPR-Cas9 in maize". Plant Cell Reports (2019)
PMID: 30684023


Raymond O, Gouzy J, Just J, Badouin H, Verdenaud M, Lemainque A, Vergne P, Moja S, Choisne N, Pont C, Carrère S, Caissard JC, Couloux A, Cottret L, Aury JM, Szécsi J, Latrasse D, Madoui MA, François L, Fu X, Yang SH, Dubois A, Piola F, Larrieu A, Perez M, Labadie K, Perrier L, Govetto B, Labrousse Y, Villand P, Bardoux C, Boltz V, Lopez-Roques C, Heitzler P, Vernoux T, Vandenbussche M, Quesneville H, Boualem A, Bendahmane A, Liu C, Le Bris M, Salse J, Baudino S, Benhamed M, Wincker P, Bendahmane M. "The Rosa genome provides new insights into the domestication of modern roses.". Nature Genetics 6 772-777 (2018)
PMID: 29713014

Francois, L., Verdenaud, M., Fu, X., Ruleman, D., Dubois, A., Vandenbussche, M., Bendahmane, A., Raymond, O., Just, J., and Bendahmane, M. "A miR172 target-deficient AP2-like gene correlates with the double flower phenotype in roses.". Sci Rep 8 12912 (2018)


"González-Carranza ZH, Peters JL, Zhang X,...". (2017)
González-Carranza ZH, Peters JL, Zhang X, Boltz V, Szecsi J, Bendahmane M, Roberts JA. HAWAIIAN SKIRT controls floral organ number and size in Arabidopsis by altering transcript levels of CUC1 and CUC2 via miR164. PLoS ONE 12(9): e0185106.

"Kong W, Yang S, Wang Y, Bendahmane M, Fu X....". (2017)
Kong W, Yang S, Wang Y, Bendahmane M, Fu X. (2017) Genome-wide identification and characterization of aquaporin gene family in Beta vulgaris. PeerJ 5:e3747

"Zhang X, Jayaweera D, Peters JL, Szecsi J,...". (2017)
Zhang X, Jayaweera D, Peters JL, Szecsi J, Bendahmane M, Roberts J, González-Carranza ZH. The Arabidopsis thaliana F-box gene HAWAIIAN SKIRT is a new player in the microRNA pathway. PLoSONE in press)

"Betsch L, Savarin J, Bendahmane M*, Szecsi J*...". (2017)
Betsch L, Savarin J, Bendahmane M*, Szecsi J* (2017) Roles of the Translationally Controlled Tumor Protein (TCTP) in Plant Development. In TCTP/tpt1 - Remodeling Signaling from Stem Cell to Disease, Vol 64. Ed. A. Telerman, R. Amson. Nature Springer publishing pp149-172.* Co-corresponding authors


"Yan H, Zhang H, Wang Q, Jian H, Qiu X,...". (2016)
Yan H, Zhang H, Wang Q, Jian H, Qiu X, Baudino S, Just J, Raymond O, Gu L, Wang J, Bendahmane M*, Tang K* (2016). The Rosa chinensis cv. Viridiflora phyllody phenotype is associated with misexpression of flower organ identity genes. Front Plant Sci. 7: 996. * Co-corresponding authors


"O. Raymond, J. Just, A. Dubois, P. Vergne, J....". (2015)
O. Raymond, J. Just, A. Dubois, P. Vergne, J. Szecsi, M. Bendahmane (2015). Rose genomics: challenges and perspectives. Acta Horticulturae 1087: XXV International EUCARPIA Symposium Section Ornamentals. pp35-40.

"Digiuni A, Berne-Dedieu A, Martinez-Torres C,...". (2015)
Digiuni A, Berne-Dedieu A, Martinez-Torres C, Szecsi J, Bendahmane M, Arneodo A, Argoul F. (2015) Single Cell Wall Nonlinear Mechanics Revealed by a Multiscale Analysis of AFM Force-Indentation Curves. Biophys J. 108(9): 2235-48.

"Magnard JL, Roccia A, Caissard JC, Vergne P,...". (2015)
Magnard JL, Roccia A, Caissard JC, Vergne P, Sun P, Hecquet R, Dubois A, Hibrand-Saint Oyant L, Jullien F, Nicolè F, Raymond O, Huguet S, Baltenweck R, Meyer S, Claudel P, Jeauffre J, Rohmer M, Foucher F, Hugueney P*, Bendahmane M*, Baudino S* (2015) Biosynthesis of monoterpene scent compounds in roses. Science. Jul 3;349(6243): 81-3. * Co-corresponding authors


Durand-Smet P, Chastrette N, Guiroy A, Richert A, Berne-Dedieu A, Szecsi J, Boudaoud A, Frachisse JM, Bendahmane M, Hamant O, Asnacios A. "A comparative mechanical analysis of plant and animal cells reveals convergence across kingdoms". Biophys J. 107 2237-44 (2014)
PMID: 25418292

"Yan, H, Zhang, H, Chen M, Jiana H, Baudino S,...". (2014)
Yan, H, Zhang, H, Chen M, Jiana H, Baudino S, Caissard JC, Bendahmane M, Lia S, Zhang T, Wang Q, Qiu Q, Zhou N, Tanga K (2014). Transcriptome and gene expression analysis during flower blooming in Rosa chinensis ‘Pallida’. GENE 540: 96-103

"Durand-Smet P, Chastrette N, Guiroy A,...". (2014)
Durand-Smet P, Chastrette N, Guiroy A, Richert A, Berne-Dedieu A, Szecsi J, Boudaoud A, Frachisse JM, Bendahmane M, Hamant O, Asnacios A. (2014) A mechanical analysis of plant cells and animal cells reveals convergence across kingdoms. Biophys J. 107(10):2237-44.

"Szécsi J, Wippermann B & Bendahmane M. (2014)...". (2014)
Szécsi J, Wippermann B & Bendahmane M. (2014) Genetic and phenotypic analyses of petal development in Arabidopsis. In Methods In Molecular Biology - Flower Development, Ed. F. Wellmer, JL Riechmann. Springer Humana press. Methods Mol Biol. 1110: 191-202.


"Bendahmane M, Dubois A, Raymond O, Le Bris M...". (2013)
Bendahmane M, Dubois A, Raymond O, Le Bris M (2013) Genetics and genomics of flower initiation and development in roses. J. Exp .Bot Feb;64(4):847-57. doi: 10.1093/jxb/ers387


"Dubois A, Carrere S, Raymond O, Pouvreau B,...". (2012)
Dubois A, Carrere S, Raymond O, Pouvreau B, Cottret L, Roccia A, Onesto JP, Sakr S, Atanassova R, Baudino S, Foucher F, Le Bris M, Gouzy J, Bendahmane M. (2012) Transcriptome database resource and gene expression atlas for the rose. BMC Genomics 13:638


"Smulders MJM, Arens P, Koning-Boucoiran CFS,...". (2011)
Smulders MJM, Arens P, Koning-Boucoiran CFS, Gitonga VW, Krens F, Atanassov A, Atanassov I, Rusanov KE, Bendahmane M, Dubois A, Raymond O, Caissard JC, Baudino S, Crespel L, Gudin S, Ricci SC, Kovatcheva N, Van Huylenbroeck J, Leus L, Wissemann V, Zimmermann H, Hensen I, Werlemark G, Nybom H. (2011) Genomic and Breeding Resources: Plantation and Ornamental Crops, Rose. C. Kole (ed.), Wild Crop Relatives: Genomic and Breeding Resources Plantation and Ornamental Crops , DOI 10.1007/978-3 -642-21201-7_12, # Springer-Verlag Berlin Heidelberg.

"Varaud E, Brioudes F, Szecsi J, Leroux J,...". (2011)
Varaud E, Brioudes F, Szecsi J, Leroux J, Brown S, Perrot-Rechenmann C and Bendahmane M (2011). AUXIN RESPONSE FACTOR8 regulates Arabidopsis petal growth by interacting with the bHLH transcription factor BIGPETALp. The Plant Cell 23: 973–983.

"Dubois A, Remay A, Raymond O, Balzergue S,...". (2011)
Dubois A, Remay A, Raymond O, Balzergue S, Chauvet A, Maene M, Pécrix Y, Yang SH, Jeauffre J, Thouroude T, Boltz V, Martin-Magniette M-L, Jancza rski S, Legeai F, Renou JP, Vergne P, Le Bris M, Foucher F and Bendahm ane (2011). Genomic Approach to Study Floral Development Genes in Rosa sp. PLoS ONE 6(12): e28455. doi:10.1371/journal.pone.0028455


"Vergne P, Maene M, Chauvet A, Debener T and...". (2010)
Vergne P, Maene M, Chauvet A, Debener T and Bendahmane M. (2010) Versatile somatic embryogenesis systems and transformation methods for the diploid rose genotype Rosa chinensis cv Old Blush. Plant Cell Tiss Organ Cult 100: 73–81

"Dubois A, Raymond O, Maene M, Baudino S,...". (2010)
Dubois A, Raymond O, Maene M, Baudino S, Langlade NB, Boltz V, Vergne P and Bendahmane M (2010) Tinkering with the C-function: A molecular frame for the selection of double flowers in cultivated roses. PLoS ONE 5(2): e9288. doi:10.1371/journal.pone.0009288


"Kheyr-Pour, A., Bendahmane, M., Matzeit, V.,...". (2009)
Kheyr-Pour, A., Bendahmane, M., Matzeit, V., Accotto, G.P., Crespi, S. and Gronenborn, B. (1991) Tomato yellow leaf curl virus from Sardinia is a whitefly-transmitted monopartite geminivirus. Nucl. Acids Res. 19 : 6763-6769.

"Bendahmane M., Schalk, H.-J. and Gronenborn...". (2009)
Bendahmane M., Schalk, H.-J. and Gronenborn B. (1995). Identification and characterization of Wheat Dwarf Virus from France using a rapid method for geminivirus DNA preparation. Phytopathology 95: 1449-1455.

"Bendahmane M. and Gronenborn B. (1997)....". (2009)
Bendahmane M. and Gronenborn B. (1997). Engineering resistance against tomato yellow leaf curl virus (TYLCV) using antisense RNA. Plant Mol. Biol. 33: 351-357.

"Bendahmane M., Fitchen J.H., Zang G. and...". (2009)
Bendahmane M., Fitchen J.H., Zang G. and Beachy R.N. (1997) Studies of coat protein-mediated resistance to tobacco mosaic tobamovirus: Correlation between assembly of mutant coat protein and resistance. J. Virology 71: 7942-7950.

"*Lewis KJ, *Bendahmane M, Smith TJ, Beachy...". (2009)
*Lewis KJ, *Bendahmane M, Smith TJ, Beachy RN, Siuzdak, G (1998) Identification of viral mutants by mass spectrometry. Proc Nat Acad Sci USA 95: 8596-8601 (*First co-authors).

"Bendahmane M, Beachy RN (1999) Control of...". (2009)
Bendahmane M, Beachy RN (1999) Control of tobamovirus infection via pathogen-derived resistance. Advances in Virus Research 53: 369-386

"Bendahmane M, Koo M, Karrer E, Beachy RN...". (2009)
Bendahmane M, Koo M, Karrer E, Beachy RN (1999) Display of epitopes on the suface if tobacco mosaic virus: Impact of charge and isoelectric point of the epitope on virus-host interactions. J Mol Biol 290: 9-20

"*Koo M, *Bendahmane M, Latteirri G, Paoletti...". (2009)
*Koo M, *Bendahmane M, Latteirri G, Paoletti E, Fitchen J, Buchmeier M, Beachy RN (1999) Protective immunity against murine hepatitis virus induced by intranasal and subcutaneous administration of hybrids of tobacco mosaic virus that carries and MHV epitope. Proc Nat Acad Sci USA 96: 7774-7779 (*First co-authors).

"Szecsi J, Ding X, Lim CO, Bendahmane M, MJ...". (2009)
Szecsi J, Ding X, Lim CO, Bendahmane M, MJ Cho, Nelson RS, RN Beachy (1999) Development of the tobacco mosaic virus infection site in Nicotiana benthamiana. Mol Plant-Microbe Interact 12: 143-152

"Gilleland HE, Gilleland LB, Staczek J, Harty...". (2009)
Gilleland HE, Gilleland LB, Staczek J, Harty RN, Garcia-Sastre A, Palese P, Brennan FR, Hamilton WDO, Bendahmane M, Beachy RN (2000) Chimeric animal and plant viruses expressing epitopes of outer membrane protein F as a combined vaccine against Pseudomonas aeruginosa lung infection. FEMS Immunology and Medical Microbiology 27: 291-297

"Staczek J, Bendahmane M, Gilleland LB, Beachy...". (2009)
Staczek J, Bendahmane M, Gilleland LB, Beachy RN, Gilleland HJ (2000) Immunization with a chimeric Tobacco Mosaic Virus containing an epitope of Outer Membrane protein F of Pseudomonas aeruginosa provides protection against challenge with P. aeruginosa. Vaccine 18: 2266-2274

"Bendahmane M, Szecsi J, Chen I, Berg HG,...". (2009)
Bendahmane M, Szecsi J, Chen I, Berg HG, Beachy RN (2002) Characterization of mutant tobacco mosaic virus coat protein which interferes with virus cell to cell movement. Proc. Natl. Acad. Sci. USA 99: 3645–3650

"Scalliet G., Journot N., Jullien F., Baudino...". (2009)
Scalliet G., Journot N., Jullien F., Baudino S., Magnard J-L., Channeliere S., Vergne P., Dumas C., Bendahmane M., Cock J.M., Hugueney P. (2002) Biosynthesis of the major scent components 3,5-dimethoxytoluene and 1,3,5-trimethoxybenzene by novel rose O-methyltransferases. FEBS Letters 523: 113-118.

"Rieseberg L.H., Raymond O., Rosenthal D.M.,...". (2009)
Rieseberg L.H., Raymond O., Rosenthal D.M., Lai Z., Livingstone K., Nakazato T., Durphy J.L., Schwarzbach A.E., Donovan L.A. and Lexer C. (2003). Major ecological transitions in wild sunflowers facilitated by hybridization. Science 301: 1211-1216.

"Lefkir Y., De Chassey B., Dubois A., Bogdanovic". (2009)
Lefkir Y., De Chassey B., Dubois A., Bogdanovic A., Brady RJ., Destaing O., Bruckert F.,O’Halloran TJ., Cosson P., Letourneur F. (2003) The AP-1 Clathrin-adaptor Is Required for LysosomalEnzymes Sorting and Biogenesis of the Contractile Vacuole Complex in Dictyostelium Cells. Mol Biol Cell. 14: 1835-51.

"Lexer C., Welch M.E., Raymond O. and Rieseberg". (2009)
Lexer C., Welch M.E., Raymond O. and Rieseberg L.H. (2003). The origin of ecological divergence in Helianthus paradoxus (Asteraceae) : selection on transgressive characters in a novel hybrid habitat. Int J Org Evolution 57: 1989-2000.

"Lexer C., Rosenthal D.M., Raymond O., Donovan...". (2009)
Lexer C., Rosenthal D.M., Raymond O., Donovan L.A., Rieseberg L.H. (2005). Genetics of species differences in the wild annual sunflowers, Helianthus annuus and Helianthus petiolaris. Genetics 169 : 2225-2239.

"Channeliere S, Riviere S, Scalliet G, Szecsi...". (2009)
Channeliere S, Riviere S, Scalliet G, Szecsi J, Jullien F, Dolle C, Vergne P, Dumas C, Bendahmane M, Hugueney P, Cock JM (2002) Analysis of gene expression in rose petals using expressed sequence tags. FEBS Letters 515: 35-38.

"Wellmer F, M Alves-Ferreira, A. Dubois, JL...". (2009)
Wellmer F, M Alves-Ferreira, A. Dubois, JL Riechmann, and EM Meyerowitz. (2006) Genome-Wide Analysis of Gene Expression during Early Arabidopsis Flower Development. PLoS Genetics, 2: 1012-1024

"Szécsi J, Joly C, Bordji K, Varaud E, Cock JM,...". (2009)
Szécsi J, Joly C, Bordji K, Varaud E, Cock JM, Dumas C, Bendahmane M. (2006). BIGPETALp, a bHLH transcription factor is involved in the control of Arabidopsis petal size. EMBO J. 25: 3912-3920.

"Bendahmane M, Chen I, Asurmendi S, Bazzini AA...". (2009)
Bendahmane M, Chen I, Asurmendi S, Bazzini AA Szecsi J, Beachy RN (2007) Coat protein-mediated resistance to TMV infection of Nicotiana tabacum involves multiple modes of interference by coat protein. Virology 366: 107-116.

"Asurmendi S, Berg RH Smith TJ, Bendahmane M,...". (2009)
Asurmendi S, Berg RH Smith TJ, Bendahmane M, Beachy RN (2007) Aggregation of TMV CP plays a role in CP functions and in coat-protein-mediated resistance. Virology 366: 98-106

"Scalliet G, Piola F, Douady CJ, Réty S,...". (2009)
Scalliet G, Piola F, Douady CJ, Réty S, Raymond O, Baudino S, Bordji K, Bendahmane M, Dumas C, Cock JM, Hugueney P (2008) Scent evolution in Chinese roses. Proc Natl Acad Sci USA 105: 5927–5932

"Brioudes F, Joly C, Szécsi J, Varaud E, Leroux...". (2009)
Brioudes F, Joly C, Szécsi J, Varaud E, Leroux J, Bellvert F, Bertrand C and Bendahmane M. (2009) Jasmonate controls late development stages of petal growth in Arabidopsis thaliana. Plant J 60: 1070–1080.

"Brioudes F, Thierry A-M, Chambrier P,...". (2009)
Brioudes F, Thierry A-M, Chambrier P, Mollereau B and Bendahmane M (2010) Translationally controlled tumor protein is a conserved mitotic growth integrator in animals and plants. Proc. Natl. Acad. Sci. USA 107: 16384-16389.

The overall objective of our group (PI M. Bendahmane) is to decipher the relationships linking gene regulation and function to plant organ morphogenesis. For these studies we mainly use the petal as a model organ (Szecsi et al., 2014). Petal traits heavily influence flower quality. They play a major role in defining the architecture of the flower thanks to their size, shape, color, scent, number and longevity. During the past few years, we focused on the investigation of the following questions: (1) How cell proliferation and expansion are regulated to attain cell-type and tissue specific values to generate organs (petals) with characteristic shapes and sizes? We use Arabidopsis thaliana to address this task. We identified a group of genes that control some aspects of early and late organ development and morphogenesis. We identified two important pathways involved in mitotic and post-mitotic growth control of petals. We explored the conserved function for one pathway between Arabirospis and Drosophila. (2) ) How important flower characters such as petal number, architecture and scent are defined?

The rose is an ideal model species to address this question. We developed genomic, transcriptomic and biotechnological tools to render the rose an ornamental model species. In parallel, we identified genes involved in the control of petal number and scent production.


- The BIGPETAL pathway to control petal morphogenesis: An interplay between jasmonate and auxin
- The TRANSLATIONALLY CONTROLLED TUMOR PROTEIN (tctp) an universal regulator of cell cycle progression during morphogenesis
- Molecular, genomic and transcriptomic approaches to understand flower initiation, development and function in Rosa sp

The BIGPETAL pathway to control petal morphogenesis: An interplay between jasmonate and auxin

Judit Szecsi, Jeremy Just, Marion Verdenaud, Jason Thomas, Claudia Bardoux

Our previous work identified a pathway involving two phytohormones to control mitotic and post-mitotic growth during petal morphogenesis. A signal initiated by jasmonates (JA), downstream of organ identity determination, controls the expression of the gene BIGPETAL (BPE; Szecsi et al., 2006) at the post-transcriptional level leading to the production of the alternative splicing variant BPEp (Brioudes et al., 2009). BPEp controls post-mitotic cell expansion by functioning in the JA pathway. BPEp interact synergistically with the AUXIN RESPONSE FACTOR8 (ARF8) to negatively control cell proliferation at early petal development stages while later during petal development BPEp and ARF interact genetically and physically to limit post mitotic cell expansion (Varaud et al., 2011). We aim at discovering the mechanisms underlying jasmonate and auxin signaling and the role of cross talks between those two phytohormones to control mitotic and post-mitotic growth during petal development. More specifically, a first goal is to address the role of JA as signal to control alternative splicing to generate BPEp and to control petal growth and (ii) to unravel the pathway initiated by JA signaling to control petal post-mitotic growth mediated by BPEp.

The TRANSLATIONALLY CONTROLLED TUMOR PROTEIN (tctp) an universal regulator of cell cycle progression during morphogenesis

Judit Szécsi, Jeremy Just, Julie Savarin, Léo Betsch, Veronique Boltz, Garance Pontier

The link between gene regulation/function and organ size and shape (morphogenesis) is poorly understood in any organism (animals like plants) and remains one of the major issues in developmental biology.
Recently, we used Arabidopsis and Drosophila as models to investigate the biological role of TRANSLATIONALLY CONTROLLED TUMOR PROTEIN (TCTP) and tested the functional conservation between animals and plants. TCTP has been known for a decade to be associated with many human cancers, but its function remains largely uncharacterized. Our study demonstrated that TCTP acts as a positive regulator of mitotic growth by specifically controlling the cell cycle progression at G1/S transition (Brioudes et al., 2010). This study also highlighted that TCTP is part of a conserved growth regulatory pathway shared between plants and animals that regulate cell division. Our work also established Arabidopsis thaliana as an important model to study TCTP function and the gained knowledge may help understanding some health disorders associated with TCTP miss-expression in mammals. We aim at understanding in more details the role of TCTP during mitotic growth. More specifically, we are currently using genetic, molecular and biochemical approaches to address how TCTP controls mitotic growth by acting at cell cycle progression.

Molecular, genomic and transcriptomic approaches to understand flower initiation, development and function in Rosa sp

Olivier Raymond, Jeremy Just, Philippe Vergne, Priscilla Villand, Claudia Bardoux, Léa François, Marion Verdenaud, FU Xiaopeng, Hinda Talaron

Description of the project.
The genus Rosa belongs to the large family of the Rosaceae. Roses are of high symbolic value and have great cultural importance in different societies worldwide. Several characters, involving mainly floral quality, were selected during rose domestication (Bendahmane et al., 2013). These include recurrent flowering, flower morphogenesis (ie. double flower formation), shoot growth and branching, scent biosynthesis and emission, …etc. Some of these characters that can hardly be investigated in Arabidopsis thaliana (or at least in a limited manner), can be studied in the rose. Beside, roses have a relatively small genome size (approximately 560 Mbp) and have a short life cycle for a perennial woody plant (about one year from seeds to flowered plants) (

Rosa sp. Genomic and transcriptomic tools
During the past few years we developed essential tools to make of the rose a model ornamental species. We used the diploid Rosa chinensis cv “Old Blush”, an ancestor of modern roses that contributed several characters (recurrent flowering, scent, etc).
We established a reproducible Agrobacterium-mediated genetic transformation method for “Old Blush” (Vergne et al., 2010). The availability of transformation methods is currently being used for gene functional studies in Rosa sp.
Recently, we used a combination of Illumina and 454 high throughput sequencing technologies to generate information on Rosa sp. transcripts using RNA from various tissues and in response to biotic and abiotic stresses (Dubois et al., 2012; Yan et al., 2014)). A total of 80714 transcript clusters corresponding to at least 20997 individual rose peptides, were identified. A digital expression for each of these clusters, in organs at different development stages and under different stress conditions, was obtained. A Web interface was created that allows data interrogation ( The database provides useful information on Rosa sp. expressed genes, with thorough annotation and an overview of expression patterns for transcripts with good accuracy, and represents a valuable prerequisite to the sequencing of the rose genome.
At ENS-Lyon, we have now gathered a collection of more than 20 rose species and 30 cultivars representing the botanical sections and horticultural groups, respectively, that have been involved in rose domestication.

The tools we developed are instrumental to address flower morphogenesis in Rosa sp (see review by Bendahmane et al., 2013). We mainly focus on flower initiation (Dubois et al., 2011), floral architecture, especially on the genetic control of petal shape and number, scent biosynthesis and emission (Scalliet et al., 2002; 2006; 2008; Channelière et al., 2002)) and meiosis (collaboration with M. Le Bris, IMBE, Aix-Marseille University, Marseille, France).

Rose flower initiation and double flowers formation
Currently, we use a combination of candidate gene and transcriptomic approaches to identify the molecular mechanisms that control flower initiation, development and petal number per flower in Rosa sp.
We established a framework of genes involved in flower initiation in Rosa using Affymetrix microarrays (Dubois et al., 2011) and candidate gene approaches.
While wild roses all have five petals, most cultivated roses have “double flowers” ranging from 10 petals to as many as 200. In roses, the "double flower" phenotype is associated with a dominant mutation, in the yet unknown DF (DOUBLE FLOWER) locus, which leads to a boundary shift of the rose AGAMOUS (RhAG) expression toward the center of the flower (Dubois et al., 2010). We further showed that a restriction of RhAG expression domain is the basis for selection of double flowers and that it was selected independently in the two major regions for rose domestication, China and the peri-Mediterranean areas (Dubois et al., 2010). To validate the function of selected genes we are using our rose genetic transformation protocol.

Rose scent biosynthesis and emission
Rose fragrance is made of hundreds of volatile compounds. The goal of the project is to explore the functional genomics of scent compounds biosynthesis in roses.
First we identified an orcinol O-methyltransferases (OOMT) involved in the biosynthesis of dimethoxytoluene (a volatile compound responsible for the "tea scent" of modern roses) and deciphered the evolutionary mechanism responsible for the emergence of tea scent in wild Chinese roses (Scalliet et al., FEBS Let 2002 ; Scalliet et al., PNAS 2008).
In the past few years we investigated the molecular basis of the biosynthesis of monoterpenes and 2-phenylethanol, two major scent compounds of the “typical rose scent”. So far in plants, a unique biosynthetic pathway of monoterpenes had been described, with the involvement enzymes belonging to the terpene synthase family. We addressed how monoterpenes are synthetized in roses and why some roses are scentless. We demonstrated that in roses, monoterpene biosynthesis is very original because it does not rely on terpene synthases but on a particular enzyme called a nudix hydrolase, RhNUDX1. Nudix hydrolases are found in animals, plants and bacteria but had never been associated with scent biosynthesis. Our data unravel the missing link between GPP (monoterpenes precursor) and Geraniol biosynthesis. We demonstrated that in rose, RhNUDX1 is involved in scent biosynthesis in petals through dephosphorylation of the precursors of scent molecules GPP. This work was recently published in Science (Magnard et al., 2015).
Currently, we are using genetic and transcriptomic approaches to investigate the pathways involved in major scent compounds in rose petals. This part of the work is done in collaboration with Sylvie Baudino, BVpam laboratory, St Etienne University, France.

Team members
VERGNE Philippe (IR1 INRA)
RAYMOND Olivier (MdC Lyon1)
JUST Jeremy (IR2 CNRS)
BOLTZ Véronique (TR INRA)
VILLAND Priscilla (ATP2, INRA)
FRANÇOIS Léa (PhD, ENS de Lyon)
PONTIER Garance (PhD, ENS de Lyon)
THOMAS Jason (PhD. Co-direction, University of Minnesota Twin)
BETSCH Léo (PhD, University Lyon1)
SAVARIN Julie (PhD, ENS de Lyon)

Past Members
XIAOPENG FU (Post-doc-France-China Cooperation FFSCA)
GOVETTO Benjamin (PhD. Co-direction Univ Marseille)
HARAGHI Aimen (PhD. Co-direction –IPS2- Univ Orsay
BROUDES Florian (PhD, 2010)
WIPERMANN Barbara (PhD, 2013)
VIALETTE Aurélie (Post-doc)
ROCCIA Aymeric (PhD, 2013)
GIRIN Thomas (Post-doc)
YANG Shu-Hua (Post-doc)

Articles in this section

  • Publications

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    2019 Betsh L, Boltz V, Brioudes F, Pontier G, Savarin J, Wipperman B, Chambrier P, Girard V, Tissot N, Benhamed M, Mollereau B, Cécile Raynaud C, Bendahmane M*, Szécsi J*. TCTP and CSN4 control cell cycle progression and development by regulating CULLIN1 neddylation in plants and animals. PLoS (...)