Marie-Cécile CAILLAUD (PI)

Lipid signaling in plant cell division and organogenesis

During the life cycle of any multicellular organism, cell division contributes to the generation of specialized cells, which are necessary to form tissues and carry out particular functions. Therefore, the mechanisms of cell division need to be tightly regulated, as malfunctions in their control can lead to tumour formation or developmental defects. This is particularly true in land plants, where cells cannot migrate and therefore cytokinesis is key for morphogenesis. In plant, cell division is executed in radically different manners than animals, with the appearance of new structures (cell plate, cytokinetic phragmoplast and the preprophase band (PPB) of microtubules) and the disappearance of ancestral mechanisms (cleavage, centrosomes).

A crucial feature of cell division is how cells position their division plane. It has even more significance for walled organisms, because their cells are embedded and cannot relocate. The memory of the cell division plane is maintained throughout plant cell division by a landmark at the equatorial periphery of the cell. Recent evidence shows that membrane lipids such as phosphoinositides (PIPs) are important for cell division orientation in plants. Phosphoinositides are ubiquitous regulators of signal transduction events in eukaryotic cells. They are produced by mono-, bis- and trisphosphorylation of the inositol head group of phosphatidylinositol. Due to their charged nature, phosphoinositides may create localized docking sites for proteins—for example, cytoskeletal and trafficking regulators—thereby defining specific membrane domains.

We are currently investigating the interplay between the phosphoinositides and the cytoskeleton during plant cell division using Arabidopsis and BY2-cells as models.

WE WANT TO ADRESS :

  • What is the crosstalk between the phosphoinositides at the plasma membrane and the cytoskeleton during mitosis ?
  • How the phosphoinositides-patterning, together with the cytoskeleton, are organized in dividing root cells ?
  • What is the impact of local perturbation of the phosphoinositides-patterning on cell division orientation ?
  • What are the upstream components responsible for the distribution of the phosphoinositides during cytokinesis ?

Results gained from the ongoing research will provide a better understanding of the signaling mechanisms coupling PIP-metabolism and cell division, the latest being fundamental in regulating organismal growth.

https://twitter.com/Caillaud_MC

OUR RESEARCH IS FUNDED BY :

  • ANR “INTERPLAY” (ANR-16-CE13-0021)
  • FR PROJETS EMERGENTS ENS LYON-2016
  • FR PROJETS EMERGENTS ENS LYON-2021
  • ANR “PlantScape” (ANR-20-CE13-0026-02)

GROUP MEMBERS :

  • VINCENT BAYLE (Engineer)
  • AURELIE FANGAIN (Engineer, ANR INTERPLAY)
  • ALEXIS LEBECQ (PhD student)
  • ELSA GASCON (Master2 student, shared with MOSAIC team)
  • CAMILA GOLDI (PostDoctorate, starting in Fall 2021)

PAST MEMBERS :

  • MEHDI DOUMANE (PhD Student- 02/2020)
  • AMELIE BAUER (Master Student in 2019)

RECENT PUBLICATIONS and PREPRINTS

2021

  • Doumane, M., Lebecq, A., Colin, L., Fangain, A., Stevens, F. D., Bareille, J., ... & Caillaud, M. C. (2021). Inducible depletion of PI(4,5)P2 by the synthetic iDePP system in Arabidopsis. Nature Plants, 7(5), 587-597.
Cutting down on phospholipids
  • Noack LC, Bayle V, Armengot L, Roer F, Mamode-Cassim A, Stevens FD, Caillaud MC, Munnik T, Mongrand S, Pleskot R, Jaillais Y. (2021) A nanodomain-anchored scaffolding complex is required for the function and localization of phosphatidylinositol 4-kinase alpha in plants. Plant Cell.
    doi : 10.1093/plcell/koab135
  • Caillaud, M. C., (in press) Methods to visualize the Actin Cytoskeleton During Plant Cell Division, (Methods in Molecular Biology), edition Plant Cell Division 10.1007/978-1-0716-1744-1
  • Caillaud, M.-C., (in press) Plant cell division : methods and protocols. Preface, (Methods in Molecular Biology), edition Plant Cell Division10.1007/978-1-0716-1744-1

2020

  • Saile, S. C., F. M. Ackermann, S. Sunil, A. Bayless, E. Stöbbe, V. Bonardi, L. Wan, M. Doumane, Y. Jaillais, M.-C. Caillaud, J. L. Dangl, M. T. Nishimura and F. El Kasmi (2020). "Coiled-coil and RPW8-type immune receptors function at the plasma membrane in a phospholipid dependent manner." bioRxiv : 2020.2011.2018.388520.
  • Noack, L., V. Bayle, L. Armengot, F. Rozier, A. Mamode-Cassim, F. Stevens, M. C. Caillaud, T. Munnik, S. Mongrand and Y. Jaillais (2020). "A nanodomain anchored-scaffolding complex is required for PI4Kα function and localization in plants." bioRxiv : 2020.2012.2008.415711.
  • Doumane, M., L. Colin, A. Lebecq, A. Fangain, J. Bareille, O. Hamant, Y. Belkhadir, Y. Jaillais and M.-C. Caillaud (2020). "iDePP : a genetically encoded system for the inducible depletion of PI(4,5)P2 in Arabidopsis thaliana." bioRxiv : 2020.2005.2013.091470.
  • Doumane, M. and M. C. Caillaud (2020). "Assessing Extrinsic Membrane Protein Dependency to PI4P Using a Plasma Membrane to Endosome Relocalization Transient Assay in Nicotiana benthamiana." Methods Mol Biol 2177 : 95-108.

2019

  • Caillaud, M.-C. (2019) Anionic Lipids : a Pipeline Connecting Key Players of Plant Cell Division. Frontiers in Plant Sciences doi:10.3389/fpls.2019.00419

2018

  • Platre, M.P., Noack, L.C., Doumane, M., Bayle, V., Simon, M.L.A., Maneta-Peyret, L., Fouillen, L., Stanislas, T., Armengot, L., Pejchar, P., Caillaud, M.C., Potocky, M., Copic, A., Moreau, P., and Jaillais, Y.2018. A combinatorial lipid code shapes the electrostatic landscape of plant endomembranes. Dev Cell 21 ;45(5):645-480.e11

2017

  • Doumane M, Lionnet C, Bayle V, Jaillais Y, Caillaud MC#. Automated Tracking of Root for Confocal Time-lapse Imaging of Cellular Processes. Bio Protoc. 2017 Apr 20 ;7(8). pii : e2245. doi : 10.21769/BioProtoc.2245. PMID : 28459086

2016

  • Simon ML*, Platre MP*, Marquès-Bueno MM, Armengot L, Stanislas T, Bayle V, Caillaud MC#, Jaillais Y#. A PtdIns(4)P-driven electrostatic field controls cell membrane identity and signalling in plants. Nature Plants. 2016 Jun 20 ;2:16089. doi : 10.1038/nplants.2016.89. PMID : 27322096

2014 and before

  • Caillaud MC*, Wirthmueller L*, Sklenar J, Findlay K, Piquerez SJ, Jones AM, Robatzek S, Jones JD, Faulkner C. The plasmodesmal protein PDLP1 localises to haustoria-associated membranes during downy mildew infection and regulates callose deposition. PLoS Pathog. 2014 Nov 13 ;10(10):e1004496.
  • Caillaud MC, Paganelli L, Lecomte P, Deslandes L, Quentin M, Pecrix Y, Le Bris M, Marfaing N, Abad P, Favery B. 2009. Spindle assembly checkpoint protein dynamics reveal conserved and unsuspected roles in plant cell division. PLoS One 4 : e6757
  • Caillaud, M. C., P. Lecomte, F. Jammes, M. Quentin, S. Pagnotta, E. Andrio, J. de Almeida Engler, N. Marfaing, P. Gounon, P. Abad and B. Favery (2008). "MAP65-3 microtubule-associated protein is essential for nematode-induced giant cell ontogenesis in Arabidopsis." Plant Cell 20(2) : 423-437.
MICROTUBULE ORGANIZATION IN NEMATODE-INDUCED GIANT CELLS

Articles de cette rubrique

  • CV Marie-Cécile Caillaud

    , par Annamaria Kiss

    Curriculum vitæ
    Family name, First name : Caillaud Marie-Cécile Researcher unique identifiers : ORCID : 0000-0002-0348-7024 Researcher ID : G-9320-2018 Date of birth : 14/10/1982 Nationality : French
    URL for web site : Cell Signalling and Endocytosis
    Education
    2019 (...)