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How stigmatic epidermis mediates the invading cell growth : the case of pollen tube and oomycete hypha

ven 26 oct 2018



Soutenance de thèse de Mme Lucie RIGLET du RDP , sous la direction de M. Thierry GAUDE

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Description générale

The epidermis is the first cellular barrier in direct contact with the environment in both animal and plant organisms. In plants, the result of the cell-to-cell communication that occurs between the pollen grain and the epidermal cells of the stigma, also called papillae, is crucial for successful reproduction. When accepted, the pollen grain germinates and emits a pollen tube that transports the male gametes towards the ovules. Effective fertilization in angiosperms depends on the proper trajectory that pollen tubes take while progressing within the pistil tissues to reach the ovules.
Pollen tubes grow within the cell wall of the papilla cells, applying pressure to the wall. Such forces are known to alter the cortical microtubule (CMT) network and cell behaviour. The first part of my PhD thesis aimed at investigating the role of the microtubule cytoskeleton of stigmatic cells in pollen tube growth. By combining cell imaging and genetic approaches, our results suggest that both organisation of CMT and cell wall properties dependent on KATANIN have a major role in guiding early pollen tube growth in stigma papillae.
Similarly to pollen tube growth within the stigmatic papilla, hypha of filamentous pathogens penetrates the epidermal tissue of the host. During pathogen attacks, epidermal cells promptly react to the invading organisms to adjust the most relevant response. Early response of the first cell layers including epidermal cells is decisive for the result of plant-pathogen interactions. The second part of my PhD work aimed at comparing the cellular response of stigmatic cells challenged by two types of invaders, the pollen tube during pollination and hyphae of two oomycete filamentous pathogens, Phytophtora parasitica and Hyaloperonospora arabidopsidis, during the infection process. We demonstrate that a stigmatic cell challenged by a pollen tube or an oomycete hypha adapts its response to the invader’s identity.

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