LOCUS       UBQ10prom::mCITRINE-1xP4M/pB7m34GW       11382 bp    DNA     circular     26-SEP-2014
DEFINITION  attB1-attB2-attB3-attB4.
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     VNTDATE|710070680|
COMMENT     VNTDBDATE|710070680|
COMMENT     LSOWNER|
COMMENT     VNTNAME|UBQ10prom::mCITRINE-1xP4M/pB7m34GW|
COMMENT     VNTAUTHORNAME|RDP-Z400-SI|
FEATURES             Location/Qualifiers
     misc_feature    10265..10285
                     /vntifkey="21"
                     /label=attB1
     misc_feature    complement(11015..11035)
                     /vntifkey="21"
                     /label=attB2
     misc_feature    complement(11375..13)
                     /vntifkey="21"
                     /label=attB3
     misc_feature    8255..8275
                     /vntifkey="21"
                     /label=attB4
     primer          8219..8235
                     /vntifkey="27"
                     /label=M13\R
     primer          22..37
                     /vntifkey="27"
                     /label=M13F
     terminator      complement(95..320)
                     /vntifkey="43"
                     /label=T35S
     misc_signal     complement(7992..8191)
                     /vntifkey="87"
                     /label=RB
     misc_marker     1839..3088
                     /vntifkey="22"
                     /label=Sm/SpR
     misc_signal     complement(1501..1833)
                     /vntifkey="87"
                     /label=LB
     misc_marker     350..1497
                     /vntifkey="22"
                     /label=Bar
                     /note="Pnos-Bar-Tnos, Plant selectable marker"
     5'UTR           9764..10263
                     /vntifkey="52"
                     /label=5'UTR
     misc_feature    9875..9959
                     /vntifkey="21"
                     /label=Exon\1.1
     intron          9960..10263
                     /vntifkey="15"
                     /label=Intron\1.1
     promoter        8278..9874
                     /vntifkey="29"
                     /label=5'\Promoter\region
     CDS             10294..11010
                     /vntifkey="4"
                     /label=mCITRINE
     misc_feature    11065..11370
                     /vntifkey="21"
                     /label=P4M
     misc_difference 11038..11064
                     /vntifkey="85"
                     /label=linker
BASE COUNT     2679 a      3058 c      2877 g      2768 t 
ORIGIN
        1 ttatacatag ttgataattc actggccgtc gttttacaac gtcgtgactg ggaaaacgat 
       61 ctaactgact agccgcggcc atggcggccg ggagcggcca tgctagagtc cgcaaaaatc 
      121 accagtctct ctctacaaat ctatctctct ctatttttct ccagaataat gtgtgagtag 
      181 ttcccagata agggaattag ggttcttata gggtttcgct catgtgttga gcatataaga 
      241 aacccttagt atgtatttgt atttgtaaaa tacttctatc aataaaattt ctaattccta 
      301 aaaccaaaat ccagtgacct gcaggtcgac catatgggag agctcgaatt atcatacatg 
      361 agaattaagg gagtcacgtt atgacccccg ccgatgacgc gggacaagcc gttttacgtt 
      421 tggaactgac agaaccgcaa cgttgaagga gccactgagc cgcgggtttc tggagtttaa 
      481 tgagctaagc acatacgtca gaaaccatta ttgcgcgttc aaaagtcgcc taaggtcact 
      541 atcagctagc aaatatttct tgtcaaaaat gctccactga cgttccataa attcccctcg 
      601 gtatccaatt agagtctcat attcactctc aactcgatcg aggggatcta ccatgagccc 
      661 agaacgacgc ccggccgaca tccgccgtgc caccgaggcg gacatgccgg cggtctgcac 
      721 catcgtcaac cactacatcg agacaagcac ggtcaacttc cgtaccgagc cgcaggaacc 
      781 gcaggagtgg acggacgacc tcgtccgtct gcgggagcgc tatccctggc tcgtcgccga 
      841 ggtggacggc gaggtcgccg gcatcgccta cgcgggtccc tggaaggcac gcaacgccta 
      901 cgactggacg gccgagtcga ccgtgtacgt ctccccccgc caccagcgga cgggactggg 
      961 ctccacgctc tacacccacc tgctgaagtc cctggaggca cagggcttca agagcgtggt 
     1021 cgctgtcatc gggctgccca acgacccgag cgtgcgcatg cacgaggcgc tcggatatgc 
     1081 cccccgcggc atgctgcggg cggccggctt caagcacggg aactggcatg acgtgggttt 
     1141 ctggcagctg gacttcagcc tgccggtgcc gccccgtccg gtcctgcccg tcaccgaaat 
     1201 ctgatgaccc ctagagtcaa gcagatcgtt caaacatttg gcaataaagt ttcttaagat 
     1261 tgaatcctgt tgccggtctt gcgatgatta tcatataatt tctgttgaat tacgttaagc 
     1321 atgtaataat taacatgtaa tgcatgacgt tatttatgag atgggttttt atgattagag 
     1381 tcccgcaatt atacatttaa tacgcgatag aaaacaaaat atagcgcgca aactaggata 
     1441 aattatcgcg cgcggtgtca tctatatgtt actagatcga ccggcatgca agctgataat 
     1501 tcaattcggc gttaattcag tacattaaaa acgtccgcaa tgtgttatta agttgtctaa 
     1561 gcgtcaattt gtttacacca caatatatcc tgccaccagc cagccaacag ctccccgacc 
     1621 ggcagctcgg cacaaaatca ccactcgata caggcagccc atcagtccgg gacggcgtca 
     1681 gcgggagagc cgttgtaagg cggcagactt tgctcatgtt accgatgcta ttcggaagaa 
     1741 cggcaactaa gctgccgggt ttgaaacacg gatgatctcg cggagggtag catgttgatt 
     1801 gtaacgatga cagagcgttg ctgcctgtga tcaattcggg cacgaaccca gtggacataa 
     1861 gcctcgttcg gttcgtaagc tgtaatgcaa gtagcgtaac tgccgtcacg caactggtcc 
     1921 agaaccttga ccgaacgcag cggtggtaac ggcgcagtgg cggttttcat ggcttcttgt 
     1981 tatgacatgt ttttttgggg tacagtctat gcctcgggca tccaagcagc aagcgcgtta 
     2041 cgccgtgggt cgatgtttga tgttatggag cagcaacgat gttacgcagc agggcagtcg 
     2101 ccctaaaaca aagttaaaca tcatggggga agcggtgatc gccgaagtat cgactcaact 
     2161 atcagaggta gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt 
     2221 gtacggctcc gcagtggatg gcggcctgaa gccacacagt gatattgatt tgctggttac 
     2281 ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg atcaacgacc ttttggaaac 
     2341 ttcggcttcc cctggagaga gcgagattct ccgcgctgta gaagtcacca ttgttgtgca 
     2401 cgacgacatc attccgtggc gttatccagc taagcgcgaa ctgcaatttg gagaatggca 
     2461 gcgcaatgac attcttgcag gtatcttcga gccagccacg atcgacattg atctggctat 
     2521 cttgctgaca aaagcaagag aacatagcgt tgccttggta ggtccagcgg cggaggaact 
     2581 ctttgatccg gttcctgaac aggatctatt tgaggcgcta aatgaaacct taacgctatg 
     2641 gaactcgccg cccgactggg ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat 
     2701 ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat 
     2761 ggagcgcctg ccggcccagt atcagcccgt catacttgaa gctagacagg cttatcttgg 
     2821 acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg tccactacgt 
     2881 gaaaggcgag atcaccaagg tagtcggcaa ataatgtcta gctagaaatt cgttcaagcc 
     2941 gacgccgctt cgccggcgtt aactcaagcg attagatgca ctaagcacat aattgctcac 
     3001 agccaaacta tcaggtcaag tctgctttta ttatttttaa gcgtgcataa taagccctac 
     3061 acaaattggg agatatatca tgcatgacca aaatccctta acgtgagttt tcgttccact 
     3121 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg 
     3181 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc 
     3241 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata 
     3301 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta 
     3361 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc 
     3421 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg 
     3481 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac 
     3541 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg 
     3601 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt 
     3661 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct 
     3721 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg 
     3781 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata 
     3841 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca 
     3901 gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc 
     3961 tgtgcggtat ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat 
     4021 agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca 
     4081 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag 
     4141 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa 
     4201 acgcgcgagg cagggtgcct tgatgtgggc gccggcggtc gagtggcgac ggcgcggctt 
     4261 gtccgcgccc tggtagattg cctggccgta ggccagccat ttttgagcgg ccagcggccg 
     4321 cgataggccg acgcgaagcg gcggggcgta gggagcgcag cgaccgaagg gtaggcgctt 
     4381 tttgcagctc ttcggctgtg cgctggccag acagttatgc acaggccagg cgggttttaa 
     4441 gagttttaat aagttttaaa gagttttagg cggaaaaatc gccttttttc tcttttatat 
     4501 cagtcactta catgtgtgac cggttcccaa tgtacggctt tgggttccca atgtacgggt 
     4561 tccggttccc aatgtacggc tttgggttcc caatgtacgt gctatccaca ggaaagagac 
     4621 cttttcgacc tttttcccct gctagggcaa tttgccctag catctgctcc gtacattagg 
     4681 aaccggcgga tgcttcgccc tcgatcaggt tgcggtagcg catgactagg atcgggccag 
     4741 cctgccccgc ctcctccttc aaatcgtact ccggcaggtc atttgacccg atcagcttgc 
     4801 gcacggtgaa acagaacttc ttgaactctc cggcgctgcc actgcgttcg tagatcgtct 
     4861 tgaacaacca tctggcttct gccttgcctg cggcgcggcg tgccaggcgg tagagaaaac 
     4921 ggccgatgcc gggatcgatc aaaaagtaat cggggtgaac cgtcagcacg tccgggttct 
     4981 tgccttctgt gatctcgcgg tacatccaat cagctagctc gatctcgatg tactccggcc 
     5041 gcccggtttc gctctttacg atcttgtagc ggctaatcaa ggcttcaccc tcggataccg 
     5101 tcaccaggcg gccgttcttg gccttcttcg tacgctgcat ggcaacgtgc gtggtgttta 
     5161 accgaatgca ggtttctacc aggtcgtctt tctgctttcc gccatcggct cgccggcaga 
     5221 acttgagtac gtccgcaacg tgtggacgga acacgcggcc gggcttgtct cccttccctt 
     5281 cccggtatcg gttcatggat tcggttagat gggaaaccgc catcagtacc aggtcgtaat 
     5341 cccacacact ggccatgccg gccggccctg cggaaacctc tacgtgcccg tctggaagct 
     5401 cgtagcggat cacctcgcca gctcgtcggt cacgcttcga cagacggaaa acggccacgt 
     5461 ccatgatgct gcgactatcg cgggtgccca cgtcatagag catcggaacg aaaaaatctg 
     5521 gttgctcgtc gcccttgggc ggcttcctaa tcgacggcgc accggctgcc ggcggttgcc 
     5581 gggattcttt gcggattcga tcagcggccg cttgccacga ttcaccgggg cgtgcttctg 
     5641 cctcgatgcg ttgccgctgg gcggcctgcg cggccttcaa cttctccacc aggtcatcac 
     5701 ccagcgccgc gccgatttgt accgggccgg atggtttgcg accgtcacgc cgattcctcg 
     5761 ggcttggggg ttccagtgcc attgcagggc cggcagacaa cccagccgct tacgcctggc 
     5821 caaccgcccg ttcctccaca catggggcat tccacggcgt cggtgcctgg ttgttcttga 
     5881 ttttccatgc cgcctccttt agccgctaaa attcatctac tcatttattc atttgctcat 
     5941 ttactctggt agctgcgcga tgtattcaga tagcagctcg gtaatggtct tgccttggcg 
     6001 taccgcgtac atcttcagct tggtgtgatc ctccgccggc aactgaaagt tgacccgctt 
     6061 catggctggc gtgtctgcca ggctggccaa cgttgcagcc ttgctgctgc gtgcgctcgg 
     6121 acggccggca cttagcgtgt ttgtgctttt gctcattttc tctttacctc attaactcaa 
     6181 atgagttttg atttaatttc agcggccagc gcctggacct cgcgggcagc gtcgccctcg 
     6241 ggttctgatt caagaacggt tgtgccggcg gcggcagtgc ctgggtagct cacgcgctgc 
     6301 gtgatacggg actcaagaat gggcagctcg tacccggcca gcgcctcggc aacctcaccg 
     6361 ccgatgcgcg tgcctttgat cgcccgcgac acgacaaagg ccgcttgtag ccttccatcc 
     6421 gtgacctcaa tgcgctgctt aaccagctcc accaggtcgg cggtggccca tatgtcgtaa 
     6481 gggcttggct gcaccggaat cagcacgaag tcggctgcct tgatcgcgga cacagccaag 
     6541 tccgccgcct ggggcgctcc gtcgatcact acgaagtcgc gccggccgat ggccttcacg 
     6601 tcgcggtcaa tcgtcgggcg gtcgatgccg acaacggtta gcggttgatc ttcccgcacg 
     6661 gccgcccaat cgcgggcact gccctgggga tcggaatcga ctaacagaac atcggccccg 
     6721 gcgagttgca gggcgcgggc tagatgggtt gcgatggtcg tcttgcctga cccgcctttc 
     6781 tggttaagta cagcgataac cttcatgcgt tccccttgcg tatttgttta tttactcatc 
     6841 gcatcatata cgcagcgacc gcatgacgca agctgtttta ctcaaataca catcaccttt 
     6901 ttagacggcg gcgctcggtt tcttcagcgg ccaagctggc cggccaggcc gccagcttgg 
     6961 catcagacaa accggccagg atttcatgca gccgcacggt tgagacgtgc gcgggcggct 
     7021 cgaacacgta cccggccgcg atcatctccg cctcgatctc ttcggtaatg aaaaacggtt 
     7081 cgtcctggcc gtcctggtgc ggtttcatgc ttgttcctct tggcgttcat tctcggcggc 
     7141 cgccagggcg tcggcctcgg tcaatgcgtc ctcacggaag gcaccgcgcc gcctggcctc 
     7201 ggtgggcgtc acttcctcgc tgcgctcaag tgcgcggtac agggtcgagc gatgcacgcc 
     7261 aagcagtgca gccgcctctt tcacggtgcg gccttcctgg tcgatcagct cgcgggcgtg 
     7321 cgcgatctgt gccggggtga gggtagggcg ggggccaaac ttcacgcctc gggccttggc 
     7381 ggcctcgcgc ccgctccggg tgcggtcgat gattagggaa cgctcgaact cggcaatgcc 
     7441 ggcgaacacg gtcaacacca tgcggccggc cggcgtggtg gtgtcggccc acggctctgc 
     7501 caggctacgc aggcccgcgc cggcctcctg gatgcgctcg gcaatgtcca gtaggtcgcg 
     7561 ggtgctgcgg gccaggcggt ctagcctggt cactgtcaca acgtcgccag ggcgtaggtg 
     7621 gtcaagcatc ctggccagct ccgggcggtc gcgcctggtg ccggtgatct tctcggaaaa 
     7681 cagcttggtg cagccggccg cgtgcagttc ggcccgttgg ttggtcaagt cctggtcgtc 
     7741 ggtgctgacg cgggcatagc ccagcaggcc agcggcggcg ctcttgttca tggcgtaatg 
     7801 tctccggttc tagtcgcaag tattctactt tatgcgacta aaacacgcga caagaaaacg 
     7861 ccaggaaaag ggcagggcgg cagcctgtcg cgtaacttag gacttgtgcg acatgtcgtt 
     7921 ttcagaagac ggctgcactg aacgtcagaa gccgactgca ctatagcagc ggaggggttg 
     7981 gatcaaagta ctttgatccc gaggggaacc ctgtggttgg catgcacata caaatggacg 
     8041 aacggataaa ccttttcacg cccttttaaa tatccgttat tctaataaac gctcttttct 
     8101 cttaggttta cccgccaata tatcctgtca aacactgata gtttaaactg aaggcgggaa 
     8161 acgacaatct gatccaagct caagctaagc ttgggcccga gcggataaca atttcacaca 
     8221 ggaaacagct atgaccatga ttacgccaag ctatcaactt tgtatagaaa agttgctagt 
     8281 ctagctcaac agagctttta acccaaattg gtacaataga atacaacttt agatcataat 
     8341 tctcaaaaga aagagattcc ttagctattc tatctgccac tccatttcct tctcggcttg 
     8401 tatgcacaag cataaaatcc tcaaacttgc taagtagata ctttatgtct tggataattg 
     8461 gattgagact tgacaagcat aactttcatg taaccaaaga cacaagttgc tgagaatcca 
     8521 cctcaaaaat gatcttccta taattgaatc gggataatga cagcacagcc catctaagag 
     8581 cctccacttc tacttccagc acgcttctta cttttaccac agctcttgca cctaaccata 
     8641 acaccttccc tgtatgatcg cgaagcaccc accctaagcc acattttaat ccttctgttg 
     8701 gccatgcccc atcaaagttg cacttaaccc aagattgtgg tggagcttcc catgtttctc 
     8761 gtctgtcccg acggtgttgt ggttggtgct ttccttacat tctgagcctc tttccttcta 
     8821 atccactcat ctgcatcttc ttgtgtcctt actaatacct cattggttcc aaattccctc 
     8881 cctttaagca ccagctcgtt tctgttcttc cacagcctcc caagtatcca agggactaaa 
     8941 gcctccacat tcttcagatc aggatattct tgtttaagat gttgaactct atggaggttt 
     9001 gtatgaactg atgatctagg accggataag ttcccttctt catagcgaac ttattcaaag 
     9061 aatgttttgt gtatcattct tgttacattg ttattaatga aaaaatatta ttggtcattg 
     9121 gactgaacac gagtgttaaa tatggaccag gccccaaata agatccattg atatatgaat 
     9181 taaataacaa gaataaatcg agtcaccaaa ccacttgcct tttttaacga gacttgttca 
     9241 ccaacttgat acaaaagtca ttatcctatg caaatcaata atcatacaaa aatatccaat 
     9301 aacactaaaa aattaaaaga aatggataat ttcacaatat gttatacgat aaagaagtta 
     9361 cttttccaag aaattcactg attttataag cccacttgca ttagataaat ggcaaaaaaa 
     9421 aacaaaaagg aaaagaaata aagcacgaag aattctagaa aatacgaaat acgcttcaat 
     9481 gcagtgggac ccacggttca attattgcca attttcagct ccaccgtata tttaaaaaat 
     9541 aaaacgataa tgctaaaaaa atataaatcg taacgatcgt taaatctcaa cggctggatc 
     9601 ttatgacgac cgttagaaat tgtggttgtc gacgagtcag taataaacgg cgtcaaagtg 
     9661 gttgcagccg gcacacacga gtcgtgttta tcaactcaaa gcacaaatac ttttcctcaa 
     9721 cctaaaaata aggcaattag ccaaaaacaa ctttgcgtgt aaacaacgct caatacacgt 
     9781 gtcattttat tattagctat tgcttcaccg ccttagcttt ctcgtgacct agtcgtcctc 
     9841 gtcttttctt cttcttcttc tataaaacaa tacccaaaga gctcttcttc ttcacaattc 
     9901 agatttcaat ttctcaaaat cttaaaaact ttctctcaat tctctctacc gtgatcaagg 
     9961 taaatttctg tgttccttat tctctcaaaa tcttcgattt tgttttcgtt cgatcccaat 
    10021 ttcgtatatg ttctttggtt tagattctgt taatcttaga tcgaagacga ttttctgggt 
    10081 ttgatcgtta gatatcatct taattctcga ttagggtttc atagatatca tccgatttgt 
    10141 tcaaataatt tgagttttgt cgaataatta ctcttcgatt tgtgatttct atctagatct 
    10201 ggtgttagtt tctagtttgt gcgatcgaat ttgtcgatta atctgagttt ttctgattaa 
    10261 caggcaagtt tgtacaaaaa agcaggctta accatggtga gcaagggcga ggagctgttc 
    10321 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc 
    10381 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc 
    10441 accaccggca agctgcccgt gccctggccc accctcgtga ccaccttcgg ctacggcctg 
    10501 atgtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg 
    10561 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc 
    10621 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc 
    10681 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac 
    10741 aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc 
    10801 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc 
    10861 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gctaccagtc caagctgagc 
    10921 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg 
    10981 atcactctcg gcatggacga gctgtacaag tacccagctt tcttgtacaa agtggctggt 
    11041 ggtagtgctt ccggactcag atccacggca agcacggaaa actttaaaaa tgttaaagaa 
    11101 aaatatcagc aaatgcgagg tgatgcttta aaaacagaaa tcctggctga tttcaaggat 
    11161 aaactggctg aagctaccga tgaacagagc cttaagcaaa ttgttgctga attgaaaagt 
    11221 aaagatgaat atagaatttt ggctaagggg caagggttaa caacccagct tttagggtta 
    11281 aagacaagtt cagtgtcttc atttgagaaa atggttgagg aaacaagaga aagtattaag 
    11341 tctcaagaaa gacaaaccat taagataaaa taagcaactt ta 
//
