LOCUS       2xCHERRY-1xMYCnoSTOP/pDONR221        4092 bp    DNA     circular     11-FEB-2011
DEFINITION  attL1-attL2.
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     VNTDATE|581600627|
COMMENT     VNTDBDATE|581600627|
COMMENT     LSOWNER|
COMMENT     VNTNAME|2xCHERRY-1xMYCnoSTOP/pDONR221|
COMMENT     VNTAUTHORNAME|RDP-Z400-SI|
FEATURES             Location/Qualifiers
     misc_feature    2435..2530
                     /vntifkey="21"
                     /label=attL1
     misc_feature    complement(4085..88)
                     /vntifkey="21"
                     /label=attL2
     terminator      2133..2160
                     /invitrogen="2590000"
                     /vntifkey="43"
                     /label=rrnB\T2\transcription\terminator
     terminator      2292..2335
                     /invitrogen="2580000"
                     /vntifkey="43"
                     /label=rrnB\T1\transcription\terminator
     promoter        complement(109..125)
                     /invitrogen="2370000"
                     /vntifkey="30"
                     /label=T7\promoter
     primer          complement(106..125)
                     /invitrogen="2100000"
                     /vntifkey="27"
                     /label=T7\primer
     primer          complement(130..146)
                     /invitrogen="2010000"
                     /vntifkey="27"
                     /label=M13\reverse\primer
     primer          2382..2398
                     /invitrogen="2000000"
                     /vntifkey="27"
                     /label=M13\(-40)\forward\primer
     primer          2402..2417
                     /invitrogen="1980000"
                     /vntifkey="27"
                     /label=M13\(-20)\forward\primer
     CDS             259..1068
                     /invitrogen="1210000"
                     /vntifkey="4"
                     /label=Kan(R)
     misc_feature    2542..4077
                     /vntifkey="21"
                     /label=2xmCHERRY-1xMyc
     misc_feature    4024..4038
                     /vntifkey="21"
                     /label=polyAlinker
     misc_feature    4048..4077
                     /vntifkey="21"
                     /label=Myc
     misc_feature    2542..4014
                     /vntifkey="21"
                     /label=2xmCHERRY
     misc_feature    2548..2571
                     /vntifkey="21"
                     /label=polyAlinker
     misc_feature    3286..3309
                     /vntifkey="21"
                     /label=polyA\linker
     misc_feature    2572..3276
                     /vntifkey="21"
                     /label=mCHERRY
     misc_feature    3310..4014
                     /vntifkey="21"
                     /label=mCHERRY
BASE COUNT      995 a      1095 c      1095 g       907 t 
ORIGIN
        1 ttgtacaaag ttggcattat aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc 
       61 actatcagtc aaaataaaat cattatttgc catccagctg atatccccta tagtgagtcg 
      121 tattacatgg tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt 
      181 tacattgcac aagataaaat aatatcatca tgaacaataa aactgtctgc ttacataaac 
      241 agtaatacaa ggggtgttat gagccatatt caacgggaaa cgtcgaggcc gcgattaaat 
      301 tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca 
      361 ggtgcgacaa tctatcgctt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat 
      421 ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg 
      481 gaatttatgc ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta 
      541 ctcaccactg cgatccccgg aaaaacagca ttccaggtat tagaagaata tcctgattca 
      601 ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt 
      661 tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg 
      721 aataacggtt tggttgatgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa 
      781 caagtctgga aagaaatgca taaacttttg ccattctcac cggattcagt cgtcactcat 
      841 ggtgatttct cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat 
      901 gttggacgag tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc 
      961 ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct 
     1021 gatatgaata aattgcagtt tcatttgatg ctcgatgagt ttttctaatc agaattggtt 
     1081 aattggttgt aacactggca gagcattacg ctgacttgac gggacggcgc aagctcatga 
     1141 ccaaaatccc ttaacgtgag ttacgcgtcg ttccactgag cgtcagaccc cgtagaaaag 
     1201 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa 
     1261 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg 
     1321 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag 
     1381 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg 
     1441 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga 
     1501 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc 
     1561 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc 
     1621 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga 
     1681 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt 
     1741 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg 
     1801 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac 
     1861 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga 
     1921 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg 
     1981 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc 
     2041 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatacgcgt 
     2101 accgctagcc aggaagagtt tgtagaaacg caaaaaggcc atccgtcagg atggccttct 
     2161 gcttagtttg atgcctggca gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt 
     2221 gcttcacaac gttcaaatcc gctcccggcg gatttgtcct actcaggaga gcgttcaccg 
     2281 acaaacaaca gataaaacga aaggcccagt cttccgactg agcctttcgt tttatttgat 
     2341 gcctggcagt tccctactct cgcgttaacg ctagcatgga tgttttccca gtcacgacgt 
     2401 tgtaaaacga cggccagtct taagctcggg ccccaaataa tgattttatt ttgactgata 
     2461 gtgacctgtt cgttgcaaca cattgatgag caatgctttt ttataatgcc aactttgtac 
     2521 aaaaaagcag gcttaaccat gctagaccct ttgcctgctg ccgctgccgc tgtgagcaag 
     2581 ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag 
     2641 ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag 
     2701 ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac 
     2761 atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc 
     2821 cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc 
     2881 gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc 
     2941 tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag 
     3001 accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc 
     3061 gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc 
     3121 acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagttg 
     3181 gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc 
     3241 cgccactcca ccggcggcat ggacgagctg tacaagggac tagacccttt gcctgctgcc 
     3301 gctgccgctg tgagcaaggg cgaggaggat aacatggcca tcatcaagga gttcatgcgc 
     3361 ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc 
     3421 gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc 
     3481 ctgcccttcg cctgggacat cctgtcccct cagttcatgt acggctccaa ggcctacgtg 
     3541 aagcaccccg ccgacatccc cgactacttg aagctgtcct tccccgaggg cttcaagtgg 
     3601 gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccctg 
     3661 caggacggcg agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc 
     3721 cccgtaatgc agaagaagac catgggctgg gaggcctcct ccgagcggat gtaccccgag 
     3781 gacggcgccc tgaagggcga gatcaagcag aggctgaagc tgaaggacgg cggccactac 
     3841 gacgctgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac 
     3901 aacgtcaaca tcaagttgga catcacctcc cacaacgagg actacaccat cgtggaacag 
     3961 tacgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta caagggacta 
     4021 gaagctgctg ctgctgctaa agctatggag caaaagctca tttctgaaga ggacttgaat 
     4081 tacccagctt tc 
//
