LOCUS       2xCHERRY-4xMyc/pDONRP2R-P3\(p3')        4352 bp    DNA     circular     17-MAR-2015
DEFINITION  attL3-attR2.
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     VNTDATE|583746335|
COMMENT     VNTDBDATE|728650182|
COMMENT     LSOWNER|
COMMENT     VNTNAME|2xCHERRY-4xMyc/pDONRP2R-P3 (p3')|
COMMENT     VNTAUTHORNAME|RDP-Z400-SI|
FEATURES             Location/Qualifiers
     misc_feature    complement(4345..88)
                     /vntifkey="21"
                     /label=attL3
     misc_feature    complement(2508..2631)
                     /vntifkey="21"
                     /label=attR2
     terminator      2152..2179
                     /invitrogen="2590000"
                     /vntifkey="43"
                     /label=rrnB\T2\transcription\terminator
     terminator      2311..2354
                     /invitrogen="2580000"
                     /vntifkey="43"
                     /label=rrnB\T1\transcription\terminator
     promoter        complement(128..144)
                     /invitrogen="2370000"
                     /vntifkey="30"
                     /label=T7\promoter
     primer          complement(125..144)
                     /invitrogen="2100000"
                     /vntifkey="27"
                     /label=T7\primer
     primer          complement(149..165)
                     /invitrogen="2010000"
                     /vntifkey="27"
                     /label=M13\reverse\primer
     primer          2401..2417
                     /invitrogen="2000000"
                     /vntifkey="27"
                     /label=M13\(-40)\forward\primer
     primer          2421..2436
                     /invitrogen="1980000"
                     /vntifkey="27"
                     /label=M13\(-20)\forward\primer
     CDS             278..1087
                     /invitrogen="1210000"
                     /vntifkey="4"
                     /label=Kan(R)
     misc_feature    2634..4340
                     /vntifkey="21"
                     /label=2xmCHERRY-4xMyc
     misc_feature    4308..4337
                     /vntifkey="21"
                     /label=Myc
     misc_feature    4269..4298
                     /vntifkey="21"
                     /label=Myc
     misc_feature    4230..4259
                     /vntifkey="21"
                     /label=Myc
     misc_feature    4191..4220
                     /vntifkey="21"
                     /label=Myc
     misc_feature    4167..4181
                     /vntifkey="21"
                     /label=polyA\linker
     misc_feature    3438..3452
                     /vntifkey="21"
                     /label=polyA\linker
     misc_feature    2700..2714
                     /vntifkey="21"
                     /label=polyA\linker
     misc_feature    4182..4340
                     /vntifkey="21"
                     /label=4xMyc
     misc_feature    3423..4159
                     /vntifkey="21"
                     /label=mCHERRY
     misc_feature    2685..3421
                     /vntifkey="21"
                     /label=mCHERRY
BASE COUNT     1073 a      1134 c      1156 g       989 t 
ORIGIN
        1 ttatacaaag ttggcattat aaaaaagcat tgcttatcaa tttgttgcaa cgaacaggtc 
       61 actatcagtc aaaataaaat cattatttgg agctccatgg tagcgttaac gcggccgcga 
      121 tatcccctat agtgagtcgt attacatggt catagctgtt tcctggcagc tctggcccgt 
      181 gtctcaaaat ctctgatgtt acattgcaca agataaaaat atatcatcat gaacaataaa 
      241 actgtctgct tacataaaca gtaatacaag gggtgttatg agccatattc aacgggaaac 
      301 gtcgaggccg cgattaaatt ccaacatgga tgctgattta tatgggtata aatgggctcg 
      361 cgataatgtc gggcaatcag gtgcgacaat ctatcgcttg tatgggaagc ccgatgcgcc 
      421 agagttgttt ctgaaacatg gcaaaggtag cgttgccaat gatgttacag atgagatggt 
      481 cagactaaac tggctgacgg aatttatgcc tcttccgacc atcaagcatt ttatccgtac 
      541 tcctgatgat gcatggttac tcaccactgc gatccccgga aaaacagcat tccaggtatt 
      601 agaagaatat cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg 
      661 gttgcattcg attcctgttt gtaattgtcc ttttaacagc gatcgcgtat ttcgtctcgc 
      721 tcaggcgcaa tcacgaatga ataacggttt ggttgatgcg agtgattttg atgacgagcg 
      781 taatggctgg cctgttgaac aagtctggaa agaaatgcat aaacttttgc cattctcacc 
      841 ggattcagtc gtcactcatg gtgatttctc acttgataac cttatttttg acgaggggaa 
      901 attaataggt tgtattgatg ttggacgagt cggaatcgca gaccgatacc aggatcttgc 
      961 catcctatgg aactgcctcg gtgagttttc tccttcatta cagaaacggc tttttcaaaa 
     1021 atatggtatt gataatcctg atatgaataa attgcagttt catttgatgc tcgatgagtt 
     1081 tttctaatca gaattggtta attggttgta acactggcag agcattacgc tgacttgacg 
     1141 ggacggcgca agctcatgac caaaatccct taacgtgagt tacgcgtcgt tccactgagc 
     1201 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat 
     1261 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga 
     1321 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt 
     1381 ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata 
     1441 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac 
     1501 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg 
     1561 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg 
     1621 tgagcattga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag 
     1681 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct 
     1741 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc 
     1801 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt 
     1861 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg 
     1921 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga 
     1981 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg 
     2041 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg 
     2101 caacgcaatt aatacgcgta ccgctagcca ggaagagttt gtagaaacgc aaaaaggcca 
     2161 tccgtcagga tggccttctg cttagtttga tgcctggcag tttatggcgg gcgtcctgcc 
     2221 cgccaccctc cgggccgttg cttcacaacg ttcaaatccg ctcccggcgg atttgtccta 
     2281 ctcaggagag cgttcaccga caaacaacag ataaaacgaa aggcccagtc ttccgactga 
     2341 gcctttcgtt ttatttgatg cctggcagtt ccctactctc gcgttaacgc tagcatggat 
     2401 gttttcccag tcacgacgtt gtaaaacgac ggccagtctt aagctcgggc cctgcagctc 
     2461 tagagctcga attctacagg tcactaatac catctaagta gttgattcat agtgactgca 
     2521 tatgttgtgt tttacagtat tatgtagtct gttttttatg caaaatctaa tttaatatat 
     2581 tgatatttat atcattttac gtttctcgtt caactttctt gtacaaagtg gctatgctcg 
     2641 aggaattctg taccccgggt tcgaaatcga taagcttgga tcctctagac cctttgcctg 
     2701 ctgccgctgc cgctgtgagc aagggcgagg aggataacat ggccatcatc aaggagttca 
     2761 tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg 
     2821 agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg 
     2881 gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc tccaaggcct 
     2941 acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc gagggcttca 
     3001 agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct 
     3061 ccctgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg 
     3121 acggccccgt aatgcagaag aagaccatgg gctgggaggc ctcctccgag cggatgtacc 
     3181 ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag gacggcggcc 
     3241 actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg 
     3301 cctacaacgt caacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg 
     3361 aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagg 
     3421 gactagaccc tttgcctgct gccgctgccg ctgtgagcaa gggcgaggag gataacatgg 
     3481 ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga gggctccgtg aacggccacg 
     3541 agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc 
     3601 tgaaggtgac caagggtggc cccctgccct tcgcctggga catcctgtcc cctcagttca 
     3661 tgtacggctc caaggcctac gtgaagcacc ccgccgacat ccccgactac ttgaagctgt 
     3721 ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga 
     3781 ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg 
     3841 gcaccaactt cccctccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct 
     3901 cctccgagcg gatgtacccc gaggacggcg ccctgaaggg cgagatcaag cagaggctga 
     3961 agctgaagga cggcggccac tacgacgctg aggtcaagac cacctacaag gccaagaagc 
     4021 ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt ggacatcacc tcccacaacg 
     4081 aggactacac catcgtggaa cagtacgaac gcgccgaggg ccgccactcc accggcggca 
     4141 tggacgagct gtacaaggga ctagaagctg ctgctgctgc taaagctatg gagcaaaagc 
     4201 tcatttctga agaggacttg aatgaaatgg agcaaaagct catttctgaa gaggacttga 
     4261 atgaaatgga gcaaaagctc atttctgaag aggacttgaa tgaaatggag caaaagctca 
     4321 tttctgaaga ggacttgaat tgagcaactt ta 
//
