LOCUS       dOCRL_(168-509)wSTOP/pD 3689 bp ds-DNA     circular     12-MAR-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     primer               complement(125..144)
                          /label="T7 primer"
                          /ApEinfo_revcolor=#b4abac
                          /ApEinfo_fwdcolor=#b4abac
     promoter_prokaryotic complement(128..144)
                          /label="T7 promoter"
                          /ApEinfo_revcolor=#75c6a9
                          /ApEinfo_fwdcolor=#75c6a9
     primer               complement(149..165)
                          /label="M13 reverse primer"
                          /ApEinfo_revcolor=#9eafd2
                          /ApEinfo_fwdcolor=#9eafd2
     CDS                  278..1087
                          /label="Kan(R)"
                          /ApEinfo_revcolor=#c7b0e3
                          /ApEinfo_fwdcolor=#c7b0e3
     terminator           2152..2179
                          /label="rrnB T2 transcription terminator"
                          /ApEinfo_revcolor=#84b0dc
                          /ApEinfo_fwdcolor=#84b0dc
     terminator           2311..2354
                          /label="rrnB T1 transcription terminator"
                          /ApEinfo_revcolor=#84b0dc
                          /ApEinfo_fwdcolor=#84b0dc
     primer               2401..2417
                          /label="M13 (-40) forward primer"
                          /ApEinfo_revcolor=#ffef86
                          /ApEinfo_fwdcolor=#ffef86
     primer               2421..2436
                          /label="M13 (-20) forward primer"
                          /ApEinfo_revcolor=#ff9ccd
                          /ApEinfo_fwdcolor=#ff9ccd
     misc_feature         complement(2508..2631)
                          /label="attR2"
                          /ApEinfo_revcolor=#ff9ccd
                          /ApEinfo_fwdcolor=#ff9ccd
     misc_feature         2634..2651
                          /label="GAGARS linker"
                          /ApEinfo_revcolor=#85dae9
                          /ApEinfo_fwdcolor=#85dae9
     misc_feature         2652..3677
                          /label="cOCRL 168-509"
                          /ApEinfo_revcolor=#d6b295
                          /ApEinfo_fwdcolor=#d6b295
     misc_feature         3246..3248
                          /label="Catalytic Asp"
                          /ApEinfo_revcolor=#ffef86
                          /ApEinfo_fwdcolor=#ffef86
     misc_feature         3462..3464
                          /label="Catalytic Arg"
                          /ApEinfo_revcolor=#c6c9d1
                          /ApEinfo_fwdcolor=#c6c9d1
     misc_feature         3480..3482
                          /label="Catalytic Asp"
                          /ApEinfo_revcolor=#ff9ccd
                          /ApEinfo_fwdcolor=#ff9ccd
     misc_feature         3483..3485
                          /label="Catalytic Arg"
                          /ApEinfo_revcolor=#b1ff67
                          /ApEinfo_fwdcolor=#b1ff67
     misc_feature         3552..3554
                          /label="catalytic D469G homolog of D523G in HsOCRL"
                          /ApEinfo_revcolor=#ffef86
                          /ApEinfo_fwdcolor=#ffef86
     misc_feature         complement(join(3682..3689,1..88))
                          /label="attL3"
                          /ApEinfo_revcolor=#f8d3a9
                          /ApEinfo_fwdcolor=#f8d3a9
     misc_feature         3689..3689
                          /label="Gateway Cloning Description"
                          /ApEinfo_revcolor=#c6c9d1
                          /ApEinfo_fwdcolor=#c6c9d1
ORIGIN
        1 ttatacaaag ttggcattat aaaaaagcat tgcttatcaa tttgttgcaa cgaacaggtc
       61 actatcagtc aaaataaaat cattatttgg agctccatgg tagcgttaac gcggccgcga
      121 tatcccctat agtgagtcgt attacatggt catagctgtt tcctggcagc tctggcccgt
      181 gtctcaaaat ctctgatgtt acattgcaca agataaaaat atatcatcat gaacaataaa
      241 actgtctgct tacataaaca gtaatacaag gggtgttatg agccatattc aacgggaaac
      301 gtcgaggccg cgattaaatt ccaacatgga tgctgattta tatgggtata aatgggctcg
      361 cgataatgtc gggcaatcag gtgcgacaat ctatcgcttg tatgggaagc ccgatgcgcc
      421 agagttgttt ctgaaacatg gcaaaggtag cgttgccaat gatgttacag atgagatggt
      481 cagactaaac tggctgacgg aatttatgcc tcttccgacc atcaagcatt ttatccgtac
      541 tcctgatgat gcatggttac tcaccactgc gatccccgga aaaacagcat tccaggtatt
      601 agaagaatat cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg
      661 gttgcattcg attcctgttt gtaattgtcc ttttaacagc gatcgcgtat ttcgtctcgc
      721 tcaggcgcaa tcacgaatga ataacggttt ggttgatgcg agtgattttg atgacgagcg
      781 taatggctgg cctgttgaac aagtctggaa agaaatgcat aaacttttgc cattctcacc
      841 ggattcagtc gtcactcatg gtgatttctc acttgataac cttatttttg acgaggggaa
      901 attaataggt tgtattgatg ttggacgagt cggaatcgca gaccgatacc aggatcttgc
      961 catcctatgg aactgcctcg gtgagttttc tccttcatta cagaaacggc tttttcaaaa
     1021 atatggtatt gataatcctg atatgaataa attgcagttt catttgatgc tcgatgagtt
     1081 tttctaatca gaattggtta attggttgta acactggcag agcattacgc tgacttgacg
     1141 ggacggcgca agctcatgac caaaatccct taacgtgagt tacgcgtcgt tccactgagc
     1201 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     1261 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     1321 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     1381 ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     1441 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     1501 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     1561 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     1621 tgagcattga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     1681 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     1741 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     1801 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     1861 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     1921 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     1981 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
     2041 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
     2101 caacgcaatt aatacgcgta ccgctagcca ggaagagttt gtagaaacgc aaaaaggcca
     2161 tccgtcagga tggccttctg cttagtttga tgcctggcag tttatggcgg gcgtcctgcc
     2221 cgccaccctc cgggccgttg cttcacaacg ttcaaatccg ctcccggcgg atttgtccta
     2281 ctcaggagag cgttcaccga caaacaacag ataaaacgaa aggcccagtc ttccgactga
     2341 gcctttcgtt ttatttgatg cctggcagtt ccctactctc gcgttaacgc tagcatggat
     2401 gttttcccag tcacgacgtt gtaaaacgac ggccagtctt aagctcgggc cctgcagctc
     2461 tagagctcga attctacagg tcactaatac catctaagta gttgattcat agtgactgca
     2521 tatgttgtgt tttacagtat tatgtagtct gttttttatg caaaatctaa tttaatatat
     2581 tgatatttat atcattttac gtttctcgtt caactttctt gtacaaagtg gctggggcag
     2641 gagccagatc cgtaaagcaa gagttaaaga agcgagagag tgagtatatc gtctataagg
     2701 acattatcat ttactgcgct acgtggaacg tgaacaataa gacatgcagt gatagtaaca
     2761 atccgctaag agcgtggctc gcatgctccg aaaagccgcc agacatatat gcgataggtc
     2821 ttcaggagct agacaccccg accaaggcta tgctcaatag tacccaagtc caagcaattg
     2881 aaaaacagtg gatagataag atgatggaca gtgtgcatcc tgacgtagag tacgagattt
     2941 tgatgtctca ccgattagtt gcgacgatgc ttactgtcat cgttagaaaa cagcttaggc
     3001 agcacattat acgttgtcga cccaaatccg tggcccgtgg aatattcaac acgctcggaa
     3061 ataagggagg ggtggcaata tccctgcagt tgaatgaggg aaacatatgt tttgttaact
     3121 cccatctggc tgcacatatg gggtacgtcg aagagaggaa tcaagattac aacgctattg
     3181 tcgaaggcat caggtttgac gacggtagaa ctatctccga ccatgaccat atattttggg
     3241 taggagactt gaattatcga atacaggaac ctccgggaca gcagcgtcct gggccgttga
     3301 gtgacgcaca gacatacgag ctcttattac aatacgatca actccgtcaa gagatgcgaa
     3361 ggggaaaatg cttcgaaggt tacacggagg gggagattaa gttcagaccg acatacaaat
     3421 atgatcctgg aacagacaat tatgactctt cagagaaaca gcgagcacct gcatactgcg
     3481 atagagtgct atggaaaggt acacgtatcg aacagctagc atacaacagc ataatggaaa
     3541 ttaggcaaag cgatcataag ccagtttatg cggttttcca ggtgaaggta aagacacgag
     3601 atgaggtgaa gtataagcgt gttcaagagg aggtgctaaa ggcggttgac aaaagggaga
     3661 acgataatca gccacagtaa gcaacttta
//