LOCUS       MP-linker-1xmCherry_wKO 4142 bp ds-DNA     circular     12-MAR-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    1356..1356
                     /label="attL2"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     operon          3261..3356
                     /label="attL1"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    complement(3334..3389)
                     /label="R-Primer"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     splicing_signal 3357..3362
                     /label="KOZAK?"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    3363..3398
                     /label="MP"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    3390..3444
                     /label="F-Primer"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    3399..3422
                     /label="polyA linker"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    3423..4130
                     /label="mCherry"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     operon          complement(join(4135..4142,1..88))
                     /label="attL2"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
ORIGIN
        1 ttgtacaaag tgggcattat aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc
       61 actatcagtc aaaataaaat cattatttgc catccagctg cagctctggc ccgtgtctca
      121 aaatctctga tgttacattg cacaagataa aaatatatca tcatgaacaa taaaactgtc
      181 tgcttacata aacagtaata caaggggtgt tatgagccat attcaacggg aaacgtcgag
      241 gccgcgatta aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa
      301 tgtcgggcaa tcaggtgcga caatctatcg cttgtatggg aagcccgatg cgccagagtt
      361 gtttctgaaa catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact
      421 aaactggctg acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga
      481 tgatgcatgg ttactcacca ctgcgatccc cggaaaaaca gcattccagg tattagaaga
      541 atatcctgat tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca
      601 ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc
      661 gcaatcacga atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg
      721 ctggcctgtt gaacaagtct ggaaagaaat gcataaactt ttgccattct caccggattc
      781 agtcgtcact catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattagg
      841 ccgggaagcc gatctcggct tgaacgaatt gttaggtggc ggtacttggg tcgatatcaa
      901 agtgcatcac ttcttcccgt atgcccaact ttgtatagag agccactgcg ggatcgtcac
      961 cgtaatctgc ttgcacgtag atcacataag caccaagcgc gttggcctca tgcttgagga
     1021 gattgatgag cgcggtggca atgccctgcc tccggtgctc gccggagact gcgagatcat
     1081 agatatagat ctcactacgc ggctgctcaa acctgggcag aacgtaagcc gcgagagcgc
     1141 caacaaccgc ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta cggagcaagt
     1201 tcccgaggta atcggagtcc ggctgatgtt gggagtaggt ggctacgtct ccgaactcac
     1261 gaccgaaaag atcaagagca gcccgcatgg atttgacttg gtcagggccg agcctacatg
     1321 tgcgaatgat gcccatactt gagccaccta actttgtttt agggcgactg ccctgctgcg
     1381 taacatcgtt gctgctgcgt aacatcgttg ctgctccata acatcaaaca tcgacccacg
     1441 gcgtaacgcg cttgctgctt ggatgcccga ggcatagact gtacaaaaaa acagtcataa
     1501 caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac
     1561 cagttgcgtg agcgcatacg ctacttgcat tacagtttac gaaccgaaca ggcttatgtc
     1621 aactgggttc gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac cttgggcagc
     1681 agcgaagtcg aggcatttct gtcctggctg gcgaacgagc gcaaggtttc ggtctccacg
     1741 catcgtcagg cattggcggc cttgctgttc ttctacggca aggtgctgtg cacggatctg
     1801 ccctggcttc aggagatcgg aagacctcgg ccgtcgcggc gcttgccggt ggtgctgacc
     1861 ccggatgaag tggttcgcat cctcggtttt ctggaaggcg agcatcgttt gttcgcccag
     1921 gactctagct atagttctag tggttggcta ctaataggtt gtattgatgt tggacgagtc
     1981 ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg tgagttttct
     2041 ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga tatgaataaa
     2101 ttgcagtttc atttgatgct cgatgagttt ttctaatcag aattggttaa ttggttgtaa
     2161 cactggcaga gcattacgct gacttgacgg gacggcgcaa gctcatgacc aaaatccctt
     2221 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     2281 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     2341 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     2401 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     2461 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     2521 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     2581 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     2641 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     2701 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     2761 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     2821 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     2881 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     2941 tatcccctga ttctgtggat aaccgtatta ccgctagcca ggaagagttt gtagaaacgc
     3001 aaaaaggcca tccgtcagga tggccttctg cttagtttga tgcctggcag tttatggcgg
     3061 gcgtcctgcc cgccaccctc cgggccgttg cttcacaacg ttcaaatccg ctcccggcgg
     3121 atttgtccta ctcaggagag cgttcaccga caaacaacag ataaaacgaa aggcccagtc
     3181 ttccgactga gcctttcgtt ttatttgatg cctggcagtt ccctactctc gcgttaacgc
     3241 tagcatggat ctcgggcccc aaataatgat tttattttga ctgatagtga cctgttcgtt
     3301 gcaacaaatt gatgagcaat gcttttttat aatgccaagt ttgtacaaaa aagcaggcta
     3361 ccatgggctt actctgcagt agaagtcgac atcatactcc tttgcctgct gccgctgccg
     3421 ctatggtgag caagggcgag gaggataaca tggccatcat caaggagttc atgcgcttca
     3481 aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg
     3541 gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt ggccccctgc
     3601 ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc
     3661 accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc aagtgggagc
     3721 gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg
     3781 acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc gacggccccg
     3841 taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac cccgaggacg
     3901 gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc cactacgacg
     3961 ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc gcctacaacg
     4021 tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg
     4081 aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag tacccagctt
     4141 tc
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