LOCUS       UBQ10prom::GVG-tE9::6xU 6498 bp ds-DNA     circular     12-MAR-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     primer               complement(188..207)
                          /label="T7 primer"
                          /ApEinfo_revcolor=#c6c9d1
                          /ApEinfo_fwdcolor=#c6c9d1
     promoter_prokaryotic complement(191..207)
                          /label="T7 promoter"
                          /ApEinfo_revcolor=#75c6a9
                          /ApEinfo_fwdcolor=#75c6a9
     primer               complement(212..228)
                          /label="M13 reverse primer"
                          /ApEinfo_revcolor=#9eafd2
                          /ApEinfo_fwdcolor=#9eafd2
     CDS                  341..1150
                          /label="Kan(R)"
                          /ApEinfo_revcolor=#c7b0e3
                          /ApEinfo_fwdcolor=#c7b0e3
     terminator           2215..2242
                          /label="rrnB T2 transcription terminator"
                          /ApEinfo_revcolor=#f8d3a9
                          /ApEinfo_fwdcolor=#f8d3a9
     terminator           2374..2417
                          /label="rrnB T1 transcription terminator"
                          /ApEinfo_revcolor=#75c6a9
                          /ApEinfo_fwdcolor=#75c6a9
     primer               2464..2480
                          /label="M13 (-40) forward primer"
                          /ApEinfo_revcolor=#d6b295
                          /ApEinfo_fwdcolor=#d6b295
     primer               2484..2499
                          /label="M13 (-20) forward primer"
                          /ApEinfo_revcolor=#ffef86
                          /ApEinfo_fwdcolor=#ffef86
     misc_feature         2540..2635
                          /label="attL4"
                          /ApEinfo_revcolor=#ffef86
                          /ApEinfo_fwdcolor=#ffef86
     promoter_eukaryotic  2638..4234
                          /label="5' Promoter region"
                          /ApEinfo_revcolor=#f8d3a9
                          /ApEinfo_fwdcolor=#f8d3a9
     5'UTR                4124..4623
                          /label="5'UTR"
                          /ApEinfo_revcolor=#b4abac
                          /ApEinfo_fwdcolor=#b4abac
     misc_feature         4235..4319
                          /label="Exon 1.1"
                          /ApEinfo_revcolor=#f58a5e
                          /ApEinfo_fwdcolor=#f58a5e
     intron               4320..4623
                          /label="Intron 1.1"
                          /ApEinfo_revcolor=#b7e6d7
                          /ApEinfo_fwdcolor=#b7e6d7
     misc_feature         4624..6489
                          /label="GVG-tE9::6xUAS-35Smini"
                          /ApEinfo_revcolor=#9eafd2
                          /ApEinfo_fwdcolor=#9eafd2
     misc_feature         4627..5925
                          /label="GVG"
                          /ApEinfo_revcolor=#d6b295
                          /ApEinfo_fwdcolor=#d6b295
     terminator           5929..6251
                          /label="tE9"
                          /ApEinfo_revcolor=#b4abac
                          /ApEinfo_fwdcolor=#b4abac
     misc_feature         6311..6479
                          /label="6xUAS-35Smini"
                          /ApEinfo_revcolor=#b4abac
                          /ApEinfo_fwdcolor=#b4abac
     misc_feature         join(6491..6498,1..116)
                          /label="attR1"
                          /ApEinfo_revcolor=#b1ff67
                          /ApEinfo_fwdcolor=#b1ff67
     misc_feature         6498..6498
                          /label="Gateway Cloning Description"
                          /ApEinfo_revcolor=#f58a5e
                          /ApEinfo_fwdcolor=#f58a5e
ORIGIN
        1 tacaaaaaag ttgaacgaga aacgtaaaat gatataaata tcaatatatt aaattagatt
       61 ttgcataaaa aacagactac ataatactgt aaaacacaac atatgcagtc actatgaatc
      121 aactacttag atggtattag tgacctgtag aattcgagct ctagagctgc agggcggccg
      181 cgatatcccc tatagtgagt cgtattacat ggtcatagct gtttcctggc agctctggcc
      241 cgtgtctcaa aatctctgat gttacattgc acaagataaa aatatatcat catgaacaat
      301 aaaactgtct gcttacataa acagtaatac aaggggtgtt atgagccata ttcaacggga
      361 aacgtcgagg ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc
      421 tcgcgataat gtcgggcaat caggtgcgac aatctatcgc ttgtatggga agcccgatgc
      481 gccagagttg tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat
      541 ggtcagacta aactggctga cggaatttat gcctcttccg accatcaagc attttatccg
      601 tactcctgat gatgcatggt tactcaccac tgcgatcccc ggaaaaacag cattccaggt
      661 attagaagaa tatcctgatt caggtgaaaa tattgttgat gcgctggcag tgttcctgcg
      721 ccggttgcat tcgattcctg tttgtaattg tccttttaac agcgatcgcg tatttcgtct
      781 cgctcaggcg caatcacgaa tgaataacgg tttggttgat gcgagtgatt ttgatgacga
      841 gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg cataaacttt tgccattctc
      901 accggattca gtcgtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg
      961 gaaattaata ggttgtattg atgttggacg agtcggaatc gcagaccgat accaggatct
     1021 tgccatccta tggaactgcc tcggtgagtt ttctccttca ttacagaaac ggctttttca
     1081 aaaatatggt attgataatc ctgatatgaa taaattgcag tttcatttga tgctcgatga
     1141 gtttttctaa tcagaattgg ttaattggtt gtaacactgg cagagcatta cgctgacttg
     1201 acgggacggc gcaagctcat gaccaaaatc ccttaacgtg agttacgcgt cgttccactg
     1261 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     1321 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     1381 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     1441 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     1501 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     1561 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     1621 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     1681 gcgtgagcat tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     1741 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     1801 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     1861 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     1921 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     1981 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     2041 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
     2101 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
     2161 gcgcaacgca attaatacgc gtaccgctag ccaggaagag tttgtagaaa cgcaaaaagg
     2221 ccatccgtca ggatggcctt ctgcttagtt tgatgcctgg cagtttatgg cgggcgtcct
     2281 gcccgccacc ctccgggccg ttgcttcaca acgttcaaat ccgctcccgg cggatttgtc
     2341 ctactcagga gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtcttccgac
     2401 tgagcctttc gttttatttg atgcctggca gttccctact ctcgcgttaa cgctagcatg
     2461 gatgttttcc cagtcacgac gttgtaaaac gacggccagt cttaagctcg ggcccgcgtt
     2521 aacgctacca tggagctcca aataatgatt ttattttgac tgatagtgac ctgttcgttg
     2581 caacaaattg ataagcaatg cttttttata atgccaactt tgtatagaaa agttgctagt
     2641 ctagctcaac agagctttta acccaaattg gtacaataga atacaacttt agatcataat
     2701 tctcaaaaga aagagattcc ttagctattc tatctgccac tccatttcct tctcggcttg
     2761 tatgcacaag cataaaatcc tcaaacttgc taagtagata ctttatgtct tggataattg
     2821 gattgagact tgacaagcat aactttcatg taaccaaaga cacaagttgc tgagaatcca
     2881 cctcaaaaat gatcttccta taattgaatc gggataatga cagcacagcc catctaagag
     2941 cctccacttc tacttccagc acgcttctta cttttaccac agctcttgca cctaaccata
     3001 acaccttccc tgtatgatcg cgaagcaccc accctaagcc acattttaat ccttctgttg
     3061 gccatgcccc atcaaagttg cacttaaccc aagattgtgg tggagcttcc catgtttctc
     3121 gtctgtcccg acggtgttgt ggttggtgct ttccttacat tctgagcctc tttccttcta
     3181 atccactcat ctgcatcttc ttgtgtcctt actaatacct cattggttcc aaattccctc
     3241 cctttaagca ccagctcgtt tctgttcttc cacagcctcc caagtatcca agggactaaa
     3301 gcctccacat tcttcagatc aggatattct tgtttaagat gttgaactct atggaggttt
     3361 gtatgaactg atgatctagg accggataag ttcccttctt catagcgaac ttattcaaag
     3421 aatgttttgt gtatcattct tgttacattg ttattaatga aaaaatatta ttggtcattg
     3481 gactgaacac gagtgttaaa tatggaccag gccccaaata agatccattg atatatgaat
     3541 taaataacaa gaataaatcg agtcaccaaa ccacttgcct tttttaacga gacttgttca
     3601 ccaacttgat acaaaagtca ttatcctatg caaatcaata atcatacaaa aatatccaat
     3661 aacactaaaa aattaaaaga aatggataat ttcacaatat gttatacgat aaagaagtta
     3721 cttttccaag aaattcactg attttataag cccacttgca ttagataaat ggcaaaaaaa
     3781 aacaaaaagg aaaagaaata aagcacgaag aattctagaa aatacgaaat acgcttcaat
     3841 gcagtgggac ccacggttca attattgcca attttcagct ccaccgtata tttaaaaaat
     3901 aaaacgataa tgctaaaaaa atataaatcg taacgatcgt taaatctcaa cggctggatc
     3961 ttatgacgac cgttagaaat tgtggttgtc gacgagtcag taataaacgg cgtcaaagtg
     4021 gttgcagccg gcacacacga gtcgtgttta tcaactcaaa gcacaaatac ttttcctcaa
     4081 cctaaaaata aggcaattag ccaaaaacaa ctttgcgtgt aaacaacgct caatacacgt
     4141 gtcattttat tattagctat tgcttcaccg ccttagcttt ctcgtgacct agtcgtcctc
     4201 gtcttttctt cttcttcttc tataaaacaa tacccaaaga gctcttcttc ttcacaattc
     4261 agatttcaat ttctcaaaat cttaaaaact ttctctcaat tctctctacc gtgatcaagg
     4321 taaatttctg tgttccttat tctctcaaaa tcttcgattt tgttttcgtt cgatcccaat
     4381 ttcgtatatg ttctttggtt tagattctgt taatcttaga tcgaagacga ttttctgggt
     4441 ttgatcgtta gatatcatct taattctcga ttagggtttc atagatatca tccgatttgt
     4501 tcaaataatt tgagttttgt cgaataatta ctcttcgatt tgtgatttct atctagatct
     4561 ggtgttagtt tctagtttgt gcgatcgaat ttgtcgatta atctgagttt ttctgattaa
     4621 cagaccatga agctactgtc ttctatcgaa caagcatgcg atatttgccg acttaaaaag
     4681 ctcaagtgct ccaaagaaaa accgaagtgc gccaagtgtc tgaagaacaa ctgggagtgt
     4741 cgctactctc ccaaaaccaa aaggtctccg ctgactaggg cacatctgac agaagtggaa
     4801 tcaaggctag aaagactgga acagctattt ctactgattt ttcctaggtc gagcgccccc
     4861 ccgaccgatg tcagcctggg ggacgagctc cacttagacg gcgaggacgt ggcgatggcg
     4921 catgccgacg cgctagacga tttcgatctg gacatgttgg gggacgggga ttccccgggt
     4981 ccgggattta ccccccacga ctccgccccc tacggcgctc tggatatggc cgacttcgag
     5041 tttgagcaga tgtttaccga tgcccttgga attgacgagt acggtgggga tccaattcag
     5101 caagccactg caggagtctc acaagacact tcggaaaatc ctaacaaaac aatagttcct
     5161 gctgcattac cacagctcac ccctaccttg gtgtcactgc tggaggtgat tgaacccgag
     5221 gtgttgtatg caggatatga tagctctgtt ccagattcag catggagaat tatgaccaca
     5281 ctcaacatgt taggtgggcg tcaagtgatt gcagcagtga aatgggcaaa ggcgatacca
     5341 ggcttcagaa acttacacct ggatgaccaa atgaccctgc tacagtactc atggatgttt
     5401 ctcatggcat ttgccctggg ttggagatca tacagacaat caagtggaaa cctgctctgc
     5461 tttgctcctg atctgattat taatgagcag agaatgtctc taccctgcat gtatgaccaa
     5521 tgtaaacaca tgctgtttgt ctcctctgaa ttacaaagat tgcaggtatc ctatgaagag
     5581 tatctctgta tgaaaacctt actgcttctc tcctcagttc ctaaggaagg tctgaagagc
     5641 caagagttat ttgatgagat tcgaatgact tatatcaaag agctaggaaa agccatcgtc
     5701 aaaagggaag ggaactccag tcagaactgg caacggtttt accaactgac aaagcttctg
     5761 gactccatgc atgaggtggt tgagaatctc cttacctact gcttccagac atttttggat
     5821 aagaccatga gtattgaatt cccagagatg ttagctgaaa tcatcactaa tcagatacca
     5881 aaatattcaa atggaaatat caaaaagctt ctgtttcatc aaaaatgact cgacctaact
     5941 gagtaagcta gcttgttcga gtattatggc attgggaaaa ctgtttttct tgtaccattt
     6001 gttgtgcttg taatttactg tgttttttat tcggttttcg ctatcgaact gtgaaatgga
     6061 aatggatgga gaagagttaa tgaatgatat ggtccttttg ttcattctca aattaatatt
     6121 atttgttttt tctcttattt gttgtgtgtt gaatttgaaa ttataagaga tatgcaaaca
     6181 ttttgttttg agtaaaaatg tgtcaaatcg tggcctctaa tgaccgaagt taatatgagg
     6241 agtaaaacac tagatcccca aacaagcttg aaactgaagg cgggaaacga caatctgatc
     6301 tctagcccat cgggtgacag ccctccgagc gggtgacagc cctccgaagc gggtgacagc
     6361 cctccgacgt cgggtgacag ccctccgagc gggtgacagc cctccgagcg ggtgacagcc
     6421 ctccgctgca gcaagaccct tcctctatat aaggaagttc atttcatttg gagaggacag
     6481 cccaagcttg caagtttg
//