{"id":2014,"date":"2015-04-29T16:34:10","date_gmt":"2015-04-29T15:34:10","guid":{"rendered":"http:\/\/www.ens-lyon.fr\/crmn\/pnmr\/?page_id=2014"},"modified":"2016-05-09T10:50:38","modified_gmt":"2016-05-09T09:50:38","slug":"training-course-florence-2016","status":"publish","type":"page","link":"https:\/\/www.ens-lyon.fr\/crmn\/pnmr\/events\/training-course-florence-2016\/","title":{"rendered":"Applied Training Courses – FLORENCE 2016"},"content":{"rendered":"

Expression and purification of metalloproteins for structural studies and<\/span><\/h1>\n

Paramagnetic restraints in hybrid methods for protein structural analysis<\/span><\/h1>\n

11th-13th May 2016, Sesto Fiorentino, Florence, Italy<\/span><\/strong>
\nRegistration deadline : <\/strong>15th April 2016<\/strong>\u00a0 <\/span><\/span>
\n\"Florence\"<\/a><\/p>\n

No fee is requested for pNMR funded fellows. <\/span><\/p>\n

Registration is opened to external participants (in limited number), but involving a registration fee (140\u20ac). Please contact us for more information.<\/span><\/p>\n


\nLunches and dinners included. Travel and accommodation borne by participants.<\/span><\/p>\n

Content:<\/span><\/h2>\n\n\n\n
The course will cover the practical aspects of :<\/span>
\n 1) recombinant expression of metalloproteins and strategies for the assembly of metallocofactors,<\/span>
\n 2) protein purification and preparation of a solution NMR sample,<\/span>
\n 3) acquisition of solution NMR spectra of paramagnetic proteins for the measurement of pseudocontact shifts and residual dipolar couplings,<\/span>
\n 4) analysis of the paramagnetic restraints for the structural characterization of proteins,<\/span>
\n 5) NMR\/X-ray hybrid structural refinement.<\/span>
\n<\/span>
\nAttendees will gain hands-on experience on the expression and preparation of an NMR sample in a wet lab, the setup of NMR experiments, the use of software tools like FANTEN, for the analysis of the paramagnetic restraints, and REFMAC-NMR, for the protein structure refinement through X-ray and paramagnetic NMR restraints<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Location<\/span><\/h2>\n

This Applied Training Course will take place at the CERM<\/a>, Via Sacconi 6, 50019 Sesto Fiorentino (FI), Italy, on the scientific campus (Polo Universitario<\/a>) of the University of Florence.<\/span><\/p>\n

It is organised by Dr. Giacomo Parigi of CIRMMP, and Prof. Claudio Luchinat, from Giotto Biotech, one of our industrial partner.<\/span><\/p>\n

<\/span><\/p>\n

Accommodation<\/span><\/h2>\n

Accommodation is not provided. A possible accommodation in Sesto Fiorentino is Hotel Villa Stanley http:\/\/www.villastanley.it\/ Please, confirm your participation and possibly provide us the details of your accommodation asap.<\/p>\n

Program <\/span><\/h2>\n

The course will take place from 9.30 to 18.00<\/p>\n

Program <\/span><\/h2>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
<\/td>\nWednesday 11th May<\/strong><\/span><\/td>\n<\/tr>\n
9.30-9.40<\/strong><\/span><\/td>\nWelcome<\/span><\/strong><\/td>\n<\/tr>\n
9.40-11.15<\/td>\nGiottoBiotech staff<\/td>\n<\/tr>\n
<\/td>\nRecombinant protein expression: how to approach<\/em><\/td>\n<\/tr>\n
11.15-11.30<\/span><\/td>\nCoffee Break<\/span>
\n<\/strong><\/td>\n<\/tr>\n
\u00a011.30-13.00<\/td>\nGiottoBiotech staff<\/td>\n<\/tr>\n
<\/td>\nExtraction of cytochrome c from E. coli pellet<\/em><\/td>\n<\/tr>\n
\u00a013.00-14.30<\/span><\/td>\nLunch<\/span><\/td>\n<\/tr>\n
\u00a014.30-18.00<\/td>\nGiottoBiotech staff<\/td>\n<\/tr>\n
<\/td>\nExtraction of cytochrome c from E. coli pellet<\/em><\/td>\n<\/tr>\n
<\/td>\nThursday 12th May<\/strong><\/span><\/td>\n<\/tr>\n
9:30-11:00<\/td>\nM. Fragai<\/td>\n<\/tr>\n
<\/td>\nStrategies for metal exchange in metalloproteins<\/em><\/td>\n<\/tr>\n
\u00a011.00-11.15<\/td>\ncoffee break<\/td>\n<\/tr>\n
11.15-13.00<\/td>\nGiottoBiotech staff<\/td>\n<\/tr>\n
<\/td>\nPurification of cytochrome c<\/em><\/td>\n<\/tr>\n
\u00a013.00-14.30<\/span><\/td>\nLunch<\/span><\/td>\n<\/tr>\n
14.30-18.00<\/td>\nE. Ravera<\/td>\n<\/tr>\n
<\/td>\nNMR of paramagnetic molecules with practicals<\/em><\/td>\n<\/tr>\n
<\/td>\nFriday 13th May<\/strong><\/span><\/td>\n<\/tr>\n
9.30-10.45<\/td>\nV. Calderone<\/td>\n<\/tr>\n
<\/td>\nFrom protein crystals to structural refinement: a quick overview<\/em><\/td>\n<\/tr>\n
10.45-11.00<\/span><\/td>\ncoffee break<\/span><\/td>\n<\/tr>\n
11.00-12.00<\/td>\nG. Parigi<\/td>\n<\/tr>\n
<\/td>\nParamagnetic restraints for protein structure calculations<\/em><\/td>\n<\/tr>\n
\u00a012.00-13.00<\/td>\nA. Carlon<\/td>\n<\/tr>\n
<\/td>\nPracticals on FANTEN and REFMAC-NMR<\/em><\/td>\n<\/tr>\n
\u00a013.00-14.30<\/span><\/td>\nLunch<\/span><\/td>\n<\/tr>\n
14.30-16.30<\/td>\nA. Carlon<\/td>\n<\/tr>\n
<\/td>\nPracticals on FANTEN and REFMAC-NMR<\/em><\/td>\n<\/tr>\n
\u00a016.30-17.30<\/td>\nC. Luchinat<\/td>\n<\/tr>\n
<\/td>\nHow to tackle protein structural data from solution and solid state : An integrated approach<\/em><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Registration <\/span><\/h2>\n
\n\t \t

pNMR Training Florence 2016<\/h3><\/div>