MEMBRANE SURFACE CHARGES (MSC) MARKERS AND new  PI4p sensor LINES

Simon ML*, Platre MP*, Marquès-Bueno MM, Armengot L, Stanislas T, Bayle V, Caillaud MC#, Jaillais Y#. A PtdIns(4)P-driven electrostatic field controls cell membrane identity and signalling in plants. Nature Plants. 2016 Jun 20;2:16089. doi: 10.1038/nplants.2016.89. PMID: 27322096

 

Cell Signaling lab

RDP - ENS Lyon - 46 Allee d’italie

69364 Lyon cedex 07 - France

Web Site : Yvon Jaillais

The following marker lines (basta Resistant) have been deposited to the NASC:


Membrane surface charge markers (sequence in VNTI format: MSCreporter.ma4):


  1. -UBQ10prom::2xmCITRINE-8K-Farn (8+) (NASC #2017342)

                mCITRINE_8KFarn.ma4

                mCITRINE_8KFarnpDONRP2RP3.gb

                UBQ10_2xmCITRINE_8KFarn.gb

  1. -UBQ10prom::2xmCITRINE-4Q4K-Farn (4+) (NASC #2017343)

                mCITRINE_4k4QFarn.gb

  1. -UBQ10prom::2xmCITRINE-8Q-Farn (0+) (NASC #2017344)

                mCITRINE_8QFarn.gb

  1. -UBQ10prom::mCITRINE-KA1(MARK1) (NASC #2107345)

                KA1(MARK1).gb

                KA1(MARK1)pDONRP2RP3.gb

                UBQ10mCITRINE_KA1.gb


  1. -UBQ10prom::+ALPS(GCS1)-mCITRINE (basta R) (NASC #2107783)

                +ALPS(GCS1)pDONR221.gb

                UBQ10prom::GCS1(+ALPS)-mCITRINE/pB7m34GW.gb


PI4P sensor: (sequence in VNTI format: P4Msensor.ma4) (NASC #2017346)


  1. -UBQ10prom::mCITRINE-P4M(SidM)

               P4M(SiDM).gb

               P4M(SiDM)pDONRP2RP3.gb

               UBQ10mCITRINE_P4M.gb    


Other lines and plasmids will be send upon request