LIPID SIGNALING in DEVELOPMENT AND ENVIRONMENTAL INTERACTIONS

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How individual cells communicate with each other and with the environment to shape plant architecture?


Plants are sessile organisms and therefore must constantly adapt their growth and architecture to an ever-changing environment. It is the delicate balance between developmental and environmental signals that shapes the architecture of the plants. Individual cells constantly integrate those cues into cellular output, that gives rise to new organs. Cells are equipped with receptor molecules that allow communication with other cells in the organism and detection of changes in their surrounding environment, such as changes in light quality, nutrient availability, attack by pathogens... 


The plasma membrane plays a critical role in regulating exchanges between the intra- and extracellular milieu and in controlling cell-to-cell communication. The aim of our group is to understand what makes the plasma membrane unique with regard to other membrane compartments and as such competent for signaling. We want to understand how signaling domains are being defined at the plant plasma membrane, both at the level of the cell (e.g., polar domains) but also at the micrometer scale (e.g., membrane microdomains). We also plan to address how these signaling domains evolve during the course of signaling and what are their functional roles during cell-cell communication.


Anionic phospholipids (e.g. phosphoinositides, PIPs) are minor lipids in membranes but they have a huge impact on cell signaling and organelle identity. They not only recruit proteins to specific cellular compartments, but they also deeply impact membrane biophysics properties. Furthermore, their production is tightly and dynamically regulated during development and interaction with the environment.


We want to address :


- What are the anionic lipids involved in plasma membrane organization and how?


  1. -What are the functions of anionic lipids and their interacting proteins in hormone signaling?

(e.g. brassinosteroid and auxin signaling)


  1. -How do anionic phospholipids orchestrate cell division and differentiation to control the final architecture of the plant ?


- How is anionic lipids homeostasis regulated by changes in the environment and contribute to plant adaptation?

 

PRINCIPAL INVESTIGATORS

MARIE-CECILE CAILLAUD - YVON JAILLAIS

Our research is based on cell and developmental biology approaches, such as live confocal imaging, expression pattern analysis, mutant phenotype description, genetics as well as protein biochemistry

OUR RESEARCH IS FUNDED BY:

GROUP MEMBERS:

CHRISTINE MIEGE (LECTURER)

VINCENT BAYLE (Engineer)

JOSEPH BAREILLE (POST-doc)

LAIA ARMENGOT (POST-DOC)

mehdi doumane (PHD STUDENT)

LISE NOACK (PHD STUDENT)

publications RéCENTES

Cell Signaling lab

RDP - ENS Lyon - 46 Allee d’italie

69364 Lyon cedex 07 - France

Web Site : Yvon Jaillais

CIG “RLK-negreg”

PCIG10-GA-2011-303601

ERC starting grant “APPL”

336360-APPL

Platre, M.P., Noack, L.C., Doumane, M., Bayle, V.,

Simon, M.L.A., Maneta-Peyret, L., Fouillen, L., Stanislas, T.,

Armengot, L., Pejchar, P., Caillaud, M.C., Potocky, M.,

Copic, A., Moreau, P., and Jaillais, Y.2018.

A combinatorial lipid code shapes the electrostatic

landscape of plant endomembranes. Dev Cell

21;45(5):645-480.e11


open access version on biorxiv:

A combinatorial lipid code shapes the electrostatic

landscape of plant endomembranes. bioRxiv, p.278135.


Stanislas T, Platre MP, Liu M, Rambaud-Lavigne LES,

Jaillais Y, Hamant O. A phosphoinositide map at the shoot

apical meristem in Arabidopsis thaliana.

BMC Biol. 2018 Feb 7;16(1):20.

doi: 10.1186/s12915-018-0490-y.



Gronnier J, Crowet JM, Habenstein B, Nasir MN, Bayle V, Hosy E, Platre MP, Gouguet P, Raffaele S, Martinez D, Grelard A, Loquet A, Simon-Plas F, Gerbeau-Pissot P, Der C, Bayer EM, Jaillais Y, Deleu M, Germain V, Lins L, Mongrand S. Structural basis for plant plasma membrane protein dynamics and organization into functional nanodomains. Elife. 2017 Jul 31;6. pii: e26404. doi: 10.7554/eLife.26404.


Noack LC, Jaillais Y#. Precision targeting by phosphoinositides: how PIs direct endomembrane trafficking in plants. Curr Opin Plant Biol. 2017 Dec;40:22-33. doi: 10.1016/j.pbi.2017.06.017. Epub 2017 Jul 19. Review.


Doumane M, Lionnet C, Bayle V, Jaillais Y, Caillaud MC#. Automated Tracking of Root for Confocal Time-lapse Imaging of Cellular Processes. Bio Protoc. 2017 Apr 20;7(8). pii: e2245. doi: 10.21769/BioProtoc.2245. PMID: 28459086

Bayle V, Platre MP, Jaillais Y#. Automatic Quantification of the Number of Intracellular Compartments in Arabidopsis thaliana Root Cells. Bio Protoc. 2017 Feb 20;7(4). pii: e2145. doi: 10.21769/BioProtoc.2145.

Simon ML*, Platre MP*, Marquès-Bueno MM, Armengot L, Stanislas T, Bayle V, Caillaud MC#, Jaillais Y#. A PtdIns(4)P-driven electrostatic field controls cell membrane identity and signalling in plants. Nature Plants. 2016 Jun 20;2:16089. doi: 10.1038/nplants.2016.89. PMID: 27322096


Armengot L*, Marquès-Bueno MM*, Jaillais Y#. Regulation of polar auxin transport by protein and lipid kinases. J Exp Bot. 2016 May 30. pii: erw216. Darwin Review. PMID: 27242371


Marquès-Bueno MM*, Morao AK*, Cayrel A*, Platre MP, Barberon M, Caillieux E, Colot V, Jaillais Y#, Roudier F#, Vert G#. A versatile Multisite Gateway-compatible promoter and transgenic line collection for cell type-specific functional genomics in Arabidopsis. Plant J. 2016 Jan;85(2):320-33. doi: 10.1111/tpj.13099. PMID: 26662936


Platre MP, Jaillais Y.
Guidelines for the Use of Protein Domains in Acidic Phospholipid Imaging.

Methods Mol Biol. 2016;1376:175-94. doi: 10.1007/978-1-4939-3170-5_15.


Belkhadir Y, Jaillais Y. The molecular circuitry of brassinosteroid signaling. New Phytol. 2015 Apr;206(2):522-40.


Martins S, Dohmann EM, Cayrel A, Johnson A, Fischer W, Pojer F, Satiat-Jeunemaître B, Jaillais Y, Chory J, Geldner N, Vert G. Internalization and vacuolar targeting of the brassinosteroid hormone receptor BRI1 are regulated by ubiquitination. Nature Communication. 2015 Jan 21;6:6151.


Simon M*, Platre M*, Assil S, van Wijk R, Chen WC, Chory J , Dreux M, Munnik T and Jaillais Y. A multi-colour/multi-affinity marker set to visualize phosphoinositide dynamics in Arabidopsis. Plant Journal, 2014 77, 322-337.


Caillaud MC*, Wirthmueller L*, Sklenar J, Findlay K, Piquerez SJ, Jones AM, Robatzek S, Jones JD, Faulkner C. The plasmodesmal protein PDLP1 localises to haustoria-associated membranes during downy mildew infection and regulates callose deposition. PLoS Pathog. 2014 Nov 13;10(10):e1004496.


Caillaud MC, Asai S, Rallapalli G, Piquerez S, Fabro G, Jones JD. A downy mildew effector attenuates salicylic Acid-triggered immunity in Arabidopsis by interacting with the host mediator complex. PLoS Biol. 2013 Dec;11(12):e1001732


Jaillais Y and Vert G. Brassinosteroids, gibberellins and light-mediated signaling are the three-way controls of plant sprouting. Nature Cell Biology. 2012 14(8), 788-790.


Youssef Belkhadir*, Yvon Jaillais*, Petra Epple, Emilia Balsemão-Pires, Jeffery L. Dangl and Joanne Chory. Brassinosteroids Modulate the Efficiency of Plant Immune Responses to Microbe-Associated Molecular Patterns. PNAS. 2012 109:297-302


Caillaud MC, Piquerez SJ, Fabro G, Steinbrenner J, Ishaque N, Beynon J, Jones JD. Subcellular localization of the Hpa RxLR effector repertoire identifies a tonoplast-associated protein HaRxL17 that confers enhanced plant susceptibility.Plant Journal. 2012 Jan;69(2):252-65.


Jaillais Y*, Belkhadir Y*, Balsemão-Pires E, Dangl JL, Chory J. Extracellular leucine-rich repeats as a platform for receptor/coreceptor complex formation. PNAS. 2011 17, 108, 8503-7.


Jaillais Y*, Hothorn M*, Belkhadir Y, Dabi T, Nimchuk ZL, Meyerowitz EM, and Chory J. Tyrosine phosphorylation controls brassinosteroid receptor activation by triggering membrane release of its kinase inhibitor. Genes and Development, 2011 25, 232-7.


Jaillais Y and Chroy J. Unraveling the paradoxes of plant hormone signaling integration. Nature Structure Molecular Biology, 2010 17, 642-645

 

ANR “INTERPLAY” ANR-16-CE13-0021

ERA CAPS “SICOPID