RESOURCES :
materials:
Protocols & methods:
Doumane M, Lionnet C, Bayle V, Jaillais Y, Caillaud MC#. Automated Tracking of Root for Confocal Time-lapse Imaging of Cellular Processes. Bio Protoc. 2017 Apr 20;7(8). pii: e2245. doi: 10.21769/BioProtoc.2245. PMID: 28459086
Sice spot detector macro for ImageJ (SiCE SpotDetector.ijm)
Wavelet A Trou plug-in for ImageJ (Wavelet_A_Trou.jar)
For testing the macro you can download image examples here
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Bayle V, Platre MP, Jaillais Y#. Automatic Quantification of the Number of Intracellular Compartments in Arabidopsis thaliana Root Cells. Bio Protoc. 2017 Feb 20;7(4). pii: e2145. doi: 10.21769/BioProtoc.2145.
Automated tracking of root for confocal time-lapse imaging of cytokinesis
You can download the three files required for automated tracking of roots here: Files_BioProtocol_Doumane.zip.
Cell Signaling lab
RDP - ENS Lyon - 46 Allee d’italie
69364 Lyon cedex 07 - France
Web Site : Yvon Jaillais
- Plant Biology - Cell Biology - Plant development - Cell Signaling - Signal transduction - Receptor Kinase - Plant adaptation - Endocytosis - Self Incompatibility - Plant Reproduction - Brassinosteroid - Plant hormones - hormone crosstalk - Intracellular trafficking - Live confocal imaging - BRI1- BKI1- SRK - SCR - Retromer - SNX - Sorting Nexin - Vacuolar Protein Sorting - VPS - RDP, ENS Lyon, Ecole Normale Superieure - Laboratoire de Reproduction et development des plantes - France -
