RESOURCES :
Protocols & methods:
Doumane M, Lionnet C, Bayle V, Jaillais Y, Caillaud MC#. Automated Tracking of Root for Confocal Time-lapse Imaging of Cellular Processes. Bio Protoc. 2017 Apr 20;7(8). pii: e2245. doi: 10.21769/BioProtoc.2245. PMID: 28459086
Sice spot detector macro for ImageJ (SiCE SpotDetector.ijm)
Wavelet A Trou plug-in for ImageJ (Wavelet_A_Trou.jar)
For testing the macro you can download image examples here
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Bayle V, Platre MP, Jaillais Y#. Automatic Quantification of the Number of Intracellular Compartments in Arabidopsis thaliana Root Cells. Bio Protoc. 2017 Feb 20;7(4). pii: e2145. doi: 10.21769/BioProtoc.2145.
Automated tracking of root for confocal time-lapse imaging of cytokinesis
You can download the three files required for automated tracking of roots here: Files_BioProtocol_Doumane.zip.
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Platre MP, Jaillais Y. " Exogenous treatment of Arabidopsis seedlings with lyso-phospholipids for the inducible complementation of lipid mutants". STAR Protoc. 2 1-13 (2021) PMID : 34223200
https://star-protocols.cell.com/protocols/749
https://github.com/mplatre/Macro_Rosette_Size/releases/tag/V1
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Bayle V*, Fiche JB*, Burny C, Platre MP, Nollmann MP, Martinière A# and Jaillais Y#. Single-particle tracking Photo-Activated Localization Microscopy of membrane proteins in living plant tissues. Nature protocols. 1600–1628 (2021). https://doi.org/10.1038/s41596-020-00471-4
● sptPALM_viewer code is available on GitHub (https://github.com/jbfiche/sptPALM_cbs), and astandalone application is freely available here: (http://bioserv.cbs.cnrs.fr/DOWNLOAD/sptPALM_data/ in the Softwares.zip file)
● MTT (http://www.ciml.univ-mrs.fr/fr/technologie/imagimm/softwares) or TrackMate (https://imagej.net/TrackMate)
● Stack_Split_macro.ijm (http://bioserv.cbs.cnrs.fr/DOWNLOAD/sptPALM_data/ in the Softwares.zip file)
● SPTpalm_Rscript.R R script (http://bioserv.cbs.cnrs.fr/DOWNLOAD/sptPALM_data/ in the
Softwares.zip file)
Cell Signaling lab
RDP - ENS Lyon - 46 Allee d’italie
69364 Lyon cedex 07 - France
Web Site : Yvon Jaillais
- Plant Biology - Cell Biology - Plant development - Cell Signaling - Signal transduction - Receptor Kinase - Plant adaptation - Endocytosis - Self Incompatibility - Plant Reproduction - Brassinosteroid - Plant hormones - hormone crosstalk - Intracellular trafficking - Live confocal imaging - BRI1- BKI1- SRK - SCR - Retromer - SNX - Sorting Nexin - Vacuolar Protein Sorting - VPS - RDP, ENS Lyon, Ecole Normale Superieure - Laboratoire de Reproduction et development des plantes - France -