The SWELLine is a collection of markers expressed in specific root tissues and that allows functional genomic in Arabidopsis thaliana, including tissue specific gene induction, nuclei purification by the INTACT method, IRES-mediated bicistronic expression and multicolor imaging.

NASC info:  http://arabidopsis.info/CollectionInfo?id=156

SWELL line seeds

The SWELL line collection is subdivided in four sets as follows:

1. •SAND lines: Cell type specific markers that label the cytoplasm (yellow) with a 4xYFP tag (Set N2106367).
   

2. •RED TIDE lines: Cell type specific markers that label the nucleus (red) with a H2B-2xCherry tag (Set N2106368).
   

3. •BREAK lines: For cell type specific nucleus purification (green) with a NTF tag (INTACT). The BREAK lines are in a ACTIN2prom::BirA background (see Deal and Henikoff 2010 Dev Cell) (Set N2106369).
   

4. •LINE-UP lines: for cell type specific inducible gene expression
   

SWELL line DNA

The SWELL line DNA collection is subdivided in four sets as follows:

1. •Each SWELL promoter cloned into L4-L1R gateway compatible vectors (this set does not include the entry vectors for the following promoters: S4, S17, S18, S29, S32, IAA19, DR5, E47, UPB1 and Q12 that were provided by Philip Benfey (Duke University, NC, USA)). Entry vectors are kanamycin resistant in bacteria. (Set N2106366)
   

2. •The destination vectors corresponding to each SAND (Set N2106363) , RED TIDE (Set N2106364) , BREAK (Set N2106365) and LINE-UP constructs. They are spectinomycin resistant in bacteria.
   

3. •The various tag used in the Marquès-Bueno et al. paper cloned in their respective gateway compatible vectors (except the entry vectors for H2B that was kindly provided by Frederic Berger (GMI, Austria)).
   

DNA sequence

Files in VNTI format

1. •Sequence of the SWELL promoter collection in entry vector > SWELLINE_entry.ma4
   

2. •Sequence of the tags used for SWELLine constructs in entry vector > tag4SWELLINE.ma4
   

Files in genebank format

1. •Promoter sequence (in .gb and pdf format): PromoterSequence.zip
   

2. •Sequence of the SWELL promoter collection in entry vector (genebank format) > SWELL_promoter_pDONRP4P1R.zip
   

3. •Sequence of the tags used for SWELLine constructs in entry vector (genebank format) > Tag4swelline.zip
   

Marquès-Bueno MM*, Morao AK*, Cayrel A*, Platre MP, Barberon M, Caillieux E, Colot V, Jaillais Y#, Roudier F#, Vert G#. A versatile Multisite Gateway-compatible promoter and transgenic line collection for cell type-specific functional genomics in Arabidopsis. Plant J. 2016 Jan;85(2):320-33. doi: 10.1111/tpj.13099.

SAND LINE: promoter::4xYFP

RED-TIDE LINE: promoter::H2B-2xCHERRY

BREAK LINE: promoter::NTF