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During development, cell-to-cell signaling through biochemical factors provides positional information for cells to acquire an identity that will determine their growth and differentiation pattern. However, organ growth also depends on mechanical interactions between cells and tissues. These interactions generate specific patterns of mechanical forces that can be sensed by cells and affect a variety of processes such as gene expression, polarity, growth and division. As mechanical stress patterns depend on organ growth and shape, mechanical forces could thus be used as signals for cells to get information on changes occurring at organ scale during morphogenesis. Still, our understanding of the contribution of mechanical signalling to plant organ morphogenesis remains poor.
In my group, we are studying how tissue mechanics regulate plant organ growth through mechanical signalling. To do so, we are using the seed of Arabidopsis thaliana as a model system to study plant organ morphogenesis. Seed growth depends on biochemical and mechanical interactions between two distinct compartments : the endosperm and the seed coat. The endosperm is a triploid zygotic compartment filling most of the inner space of the seed at early stages of development and whose turgor pressure is thought to be driving force for growth. The seed coat is composed of several layers derived from the maternal integuments of the ovule and is thought to restrict growth through cell wall stiffening thanks to perception of the tension induced in the seed coat by endosperm expansion.
Using experimental approaches and computational models developed in collaboration with Olivier Ali from the MOSAIC team (RDP), we are studying the mechanical regulation of seed growth by :
1. Investigating the contribution of endosperm turgor pressure to seed growth.
2. Studying how mechanical interactions between the endosperm and the seed coat regulate seed size and shape.
3. Charactering the genetic determinants controlling the mechanical properties and responses to forces of the different seed coat layers.
The Seed Mechanics Group is part of the Seed Development Team (SeedDev), which is led by Gwyneth Ingram at the Laboratory of Plant Development and Reproduction (RDP), To see the other activities of the SeedDev team : Click here
o Benoit Landrein, Permanent Researcher (CR-CN CNRS), CV
o Audrey Creff, Permanent Engineer (IE CNRS)
o Jeanne Braat, Post-Doc (CNRS, ANR MECHASEED)
o Runjue Yao, PhD Student (CNRS/University of Melbourne), co-supervised with Gwnyeth Ingram (CNRS) and John Golz (University of Melbourne)
Interested in joining the group ? Click here
o Camille Bied, PhD student (ENS)
o Amélie Bauer, PhD student (CNRS), co-supervised with Gwyneth Ingram (CNRS) and John Golz (University of Melbourne)
o Enzo Salou, M1 student (University of Montpellier)
o Jeanne Couderc, M1 student (ENSAIA)
o Adrien Delattre, M1 student (University Lyon I)
o Olivier Ali, MOSAIC team, RDP laboratory
o Gwyneth Ingram, Seed Development team, RDP laboratory
o John Golz, University of Melbourne (Australia)
o Duarte Figueiredo, MPIMP (Germany)
o Helene Robert, CEITEC (Czech Republic)
o Elena Baena Gonzalez, University of Oxford (UK)
o Bauer A., Bied C., Delattre A., Ingram G., Golz J.G., Landrein B (2024) Spatiotemporally distinct responses to mechanical forces shape the developing seed of Arabidopsis EmboJ. 43(13):2733-2758 https://doi.org/10.1038/s44318-024-00138-w
This work demonstrates that two distinct mechanical responses, triggered at different stages of seed development in each outer integument layer, modulate seed growth to determine both its final size and shape.
o Creff A., Ali O*, Bied C., Bayle V, Ingram GC*, Landrein B*. (2023) Evidence that endosperm turgor pressure both promotes and restricts seed growth and size. Nature Communications. 14(1):67 https://doi.org/10.1038/s41467-022-35542-5
This work, a product of collaboration with Olivier Ali, a physicist from the RDP institute, presents a model of seed morphogenesis based on the mechanical interaction between the endosperm and the seed coat. By combining experiments and modeling, this study demonstrates that endosperm pressure plays two antagonistic roles during seed development : it directly promotes growth but indirectly inhibits it by generating tension in the seed coat, which triggers mechanosensitive wall stiffening in one of the outer integument layers.
o Landrein B, Ingram G. 2019. Connected through the force : mechanical signals in plant development. Journal of Experimental Botany. https://doi.org/10.1093/jxb/erz103
o Lima RB, Pankaj R, Ehlert S, Finger P, Fröhlich A, Bayle V, Landrein B, Sampathkumar A, Figueiredo DD (2024) Seed coat-derived brassinosteroid signaling regulates endosperm development. Nature Communications. 15, 9352. https://doi.org/10.1038/s41467-024-53671-x
o Prabhullachandran U, Urbánková I, Medaglia-Mata A, Creff A, Voxeur A, Petřík I, Pěnčík A, Novák O, Landrein B, Hejátko J, Robert HS. (2023) Long-term high temperatures affect seed maturation and seed coat integrity in Brassica napus. BioRxiv. https://doi.org/10.1101/2024.11.27.625589
During my PhD and my Post-doc, I studied how mechanical and environmental signals affect the development of the shoot apical meristem, the structure generating all of the aerial organs of the plant. Here are a selection of publications related to this subject :
o Lopes L, Formosa-Jordan P, Malivert A, Margalha L, Confraria A, Feil R, Lunn J.E, Jönsson H*, Landrein B* and Baena-González E* (2024) Sugar signaling modulates SHOOT MERISTEMLESS expression and meristem function in Arabidopsis. PNAS. 121(37):e2408699121. https://www.pnas.org/doi/10.1073/pnas.2408699121
o Lopes FL, Galvan-Ampudia C, Landrein B. 2020. WUSCHEL in the shoot apical meristem : old player, new tricks. Journal of Experimental Botany. https://doi.org/10.1093/jxb/eraa572
o Landrein B, Formosa-Jordan P, Malivert A, Schuster C, Melnyk CW, Yang W, Turnbull C, Meyerowitz EM, Locke JCW, Jönsson H. 2018. Nitrate modulates stem cell dynamics in Arabidopsis shoot meristems through cytokinins. PNAS. doi : https://doi.org/10.1073/pnas.1718670115
o Gruel J, Landrein B, Tarr P, Schuster C, Refahi Y, Sampathkumar A, Hamant O, Meyerowitz EM, Jönsson H. 2016. An epidermis-driven mechanism positions and scales stem cell niches in plants. Science Advances. https://doi.org/10.1126/sciadv.1500989
o Landrein B, Kiss A, Sassi M, Chauvet A, Das P, Cortizo M, Laufs P, Takeda S, Aida M, Traas J, Vernoux T, Boudaoud A, Hamant O. 2015. Mechanical stress contributes to the expression of the STM homeobox gene in Arabidopsis shoot meristems. eLife. https://doi.org/10.7554/eLife.07811
o Landrein B, Refahi Y, Besnard F, Hervieux N, Mirabet V, Boudaoud A, Vernoux T, Hamant O. 2015. Meristem size contributes to the robustness of phyllotaxis in Arabidopsis. Journal of Experimental Botany. https://doi.org/10.1093/jxb/eru482
o Landrein B, Lathe R, Bringmann M, Vouillot C, Ivakov A, Boudaoud A, Persson S, Hamant O. 2013. Impaired cellulose synthase guidance leads to stem torsion and twists phyllotactic patterns in Arabidopsis. Current Biology. https://doi.org/10.1016/j.cub.2013.04.013
o Landrein B, Hamant O. 2013. How mechanical stress controls microtubule behavior and morphogenesis in plants : history, experiments and revisited theories. Plant Journal. https://doi.org/10.1111/tpj.12188
o Uyttewaal M, Burian A, Alim K, Landrein B, Borowska-Wykręt D, Dedieu A, Peaucelle A, Ludynia M, Traas J, Boudaoud A, Kwiatkowska D, Hamant O. 2012. Mechanical stress acts via katanin to amplify differences in growth rate between adjacent cells in Arabidopsis. Cell. https://doi.org/10.1016/j.cell.2012.02.048
Benoit Landrein (PhD) CNRS Researcher (CR-CN), Seed Mechanics Group Team Seed Development Laboratory of Plant Reproduction and Development (RDP), ENS de Lyon, France Google scholar ORCID
Employment History • Since 2018 CNRS Researcher Laboratory of Plant Reproduction and Development, ENS de (…)