The PIPline

The PIPline is a multicolor and multi affinity marker set that highlights membrane-associated phosphoinositides (PIPs) in Arabidopsis.

Seeds can be ordered as individual stocks or as a collection at ABCR (ABRC stock set #CS2105604) or NASC (NASC stock set # N21056604)

All the sequences of the PIPline entry vectors in genebank format can be found here :

All the sequences of the PIPline destination vectors in genebank format can be found here :

Please cite the following paper when using this material :
Simon M*, Platre M*, Assil S, van Wijk R, Chen WC, Chory J , Dreux M, Munnik T and Jaillais Y. 2014. A multi-colour/multi-affinity marker set to visualize phosphoinositide dynamics in Arabidopsis. Plant Journal, Jan ;77(2):322-37. doi : 10.1111/tpj.12358. PMID : 24147788

The Swelline

The SWELLine is a collection of markers expressed in specific root tissues that allow functional genomics in Arabidopsis thaliana, including tissue-specific gene induction, nuclei purification by the INTACT method, IRES-mediated bicistronic expression, and multicolor imaging.

Seeds and plasmids can be ordered as individual stocks or as a collection as detailed here (NASC).

SWELL line seeds :

The SWELL line collection is subdivided in four sets as follows :

•SAND lines : Cell type specific markers that label the cytoplasm (yellow) with a 4xYFP tag (Set N2106367).

•RED TIDE lines : Cell type-specific markers that label the nucleus (red) with an H2B-2xCherry tag (Set N2106368).

•BREAK lines : For cell type specific nucleus purification (green) with a NTF tag (INTACT). The BREAK lines are in a ACTIN2prom::BirA background (see Deal and Henikoff 2010 Dev Cell) (Set N2106369).

•LINE-UP lines : for cell type specific inducible gene expression

SWELL line DNA

The SWELL line DNA collection is subdivided in four sets as follows :

•Each SWELL promoter cloned into L4-L1R gateway compatible vectors (this set does not include the entry vectors for the following promoters : S4, S17, S18, S29, S32, IAA19, DR5, E47, UPB1 and Q12 that were provided by Philip Benfey (Duke University, NC, USA)). Entry vectors are kanamycin resistant in bacteria. (Set N2106366)

•The destination vectors corresponding to each SAND (Set N2106363) , RED TIDE (Set N2106364) , BREAK (Set N2106365) and LINE-UP constructs. They are spectinomycin resistant in bacteria.

•The various tag used in the Marquès-Bueno et al. paper cloned in their respective gateway compatible vectors (except the entry vectors for H2B that was kindly provided by Frederic Berger (GMI, Austria)).

• The promoter sequences (in .gb and pdf format) can be downloaded here :

•The sequences of the SWELL promoter collection in entry vector (genebank format) can be downloaded here :

•The sequenceq of the tags used for SWELLine constructs in entry vector (genebank format) can be downloaded here :

Please cite the following paper when using this material :
Marquès-Bueno MM*, Morao AK*, Cayrel A*, Platre MP, Barberon M, Caillieux E, Colot V, Jaillais Y#, Roudier F#, Vert G#. A versatile Multisite Gateway-compatible promoter and transgenic line collection for cell type-specific functional genomics in Arabidopsis. Plant Journal. 2016 Jan ;85(2):320-33. doi : 10.1111/tpj.13099.

Membrane surface charge sensors

The following reporter lines have been donated to the NASC (see individual NASC number)

– UBQ10prom::2xmCITRINE-8K-Farn (8+) (NASC #2017342)
– UBQ10prom::2xmCITRINE-4Q4K-Farn (4+) (NASC #2017343)
– UBQ10prom::2xmCITRINE-8Q-Farn (0+) (NASC #2017344)
– UBQ10prom::mCITRINE-KA1(MARK1) (NASC #2107345)
– UBQ10prom : :+ALPS(GCS1)-mCITRINE (basta R) (NASC #2107783)

– UBQ10prom::mCITRINE-P4M(SidM), PI4P sensor (NASC #2017346)

The sequences of the vectors used to construct the membrane charge sensors and the P4M PI4P reporter can be downloaded here :

Please cite the following paper when using this material :
Simon ML*, Platre MP*, Marquès-Bueno MM, Armengot L, Stanislas T, Bayle V, Caillaud MC#, Jaillais Y#. A PtdIns(4)P-driven electrostatic field controls cell membrane identity and signalling in plants. Nature Plants. 2016 Jun 20 ;2:16089. doi : 10.1038/nplants.2016.89. PMID : 27322096

Phosphatidylserine (PS) and phosphatidic acid (PA) sensors

The following reporter lines have been donated to the NASC (see individual NASC number)

PS sensors :

– UBQ10prom::mCITRINE-C2(Lact) (basta R) (NASC #2017347)
– UBQ10prom::2xmCHERRY-C2(Lact) (Hygro R) (NASC #2107778)
– UBQ10prom::mCITRINE-2xPH(EVECTIN2) (basta R) (NASC #2107779)
– UBQ10prom::tdTOMATO-2xPH(EVECTIN2) (hygro R) (NASC #2107780)

PA sensors

– UBQ10prom::mCITRINE-1xPASS (basta R) (NASC #2107781)
– UBQ10prom::mCITRINE-2xPASS (basta R) (NASC #2107782)

The sequences of the vectors used to construct the PS and PA sensors can be downloaded here :

Please cite the following paper when using this material :
Platre, M.P., Noack, L.C., Doumane, M., Bayle, V., Simon, M.L.A., Maneta-Peyret, L., Fouillen, L., Stanislas, T., Armengot, L., Pejchar, P., Caillaud, M.C., Potocky, M., Copic, A., Moreau, P., and Jaillais, Y.2018. A combinatorial lipid code shapes the electrostatic landscape of plant endomembranes. Dev Cell 21 ;45(5):645-480.e11

ROP6 lines and PS mutants

The following lines have been donated to the NASC (see individual NASC number)

– 2x35S::mEos-ROP6(CA) Col0 background N2109736 (bastaR)
– 2x35S::mEos-ROP6 line #1 Col0 background N2109737 (bastaR)
– 2x35S::mEos-ROP6 line #2 Col0 background N2109738 (bastaR)
– 2x35S::mEos-ROP6-7Q Col0 background N2109739 (bastaR)
– ROP6prom::mCITRINE-ROP6 in rescued rop6-2 background N2109740 (bastaR/kanR)
– ROP6prom::mCITRINE-ROP6-7Q in non-rescued rop6-2 background N2109741 bastaR/kanR)
– UBQ10prom::mCITRINE-ROP6-Cter Col0 background N2109742 (bastaR/kanR)
– UBQ10prom::mCITRINE-ROP6-Cter-3Q Col0 background N2109743 (bastaR/kanR)
– UBQ10prom::mCITRINE-ROP6-Cter-7Q Col0 background N2109744 (bastaR/kanR)
– PSS1-OX1 Col0 background N2109745 (bastaR)
– PSS1-OX2 Col0 background N2109746 (bastaR)
– ami PSS1 Col0 background N2109747 (kanaR)
– ami ! PSS1 Col0 background N2109748 (kanaR)

Please cite the following paper when using this material :
Platre, M.P., Bayle, V., Armengot, L., Bareille, J., Marques-Bueno, M.M., Creff, A., Maneta-Peyret, L., Fiche, J.B., Nolmann, M., Miège, C., Moreau, P., Martinière, A., and Jaillais, Y#. (2018). Developmental control of plant Rho GTPase nano-organization by the lipid phosphatidylserine. Science. 2019 Apr 5 ;364(6435):57-62. doi : 10.1126/science.aav9959.

PI4P and PI(4,5)P2 (iDEPP) depletion systems

The following marker lines have been deposited to the NASC :

– MAP-mCH-dOCRL Col0 background N2110785 (HygroR)
– MAP-mCH-dOCRLdead Col0 background N2110786 (HygroR)
– MAP-3xmCH Col0 background N2110787 (HygroR)
– MAP-mCH-dOCRL x mCit-2xPH(PLC) (P24Y) Col0 background N2110788 (HygroR + BastaR)
– MAP-mCH-dOCRLdead x mCit-2xPH(PLC) (P24Y) Col0 background N2110789 (HygroR + BastaR)
– MAP-3xmCH x mCit-2xPH(PLC) (P24Y) Col0 background N2110790 (HygroR + BastaR)
– MAP-mCH-dOCRL x mCit-TUBBY (P15Y) Col0 background N2110791 (HygroR + BastaR)
– MAP-mCH-dOCRLdead x mCit-TUBBY (P15Y) Col0 background N2110792 (HygroR + BastaR)
– MAP-3xmCH x mCit-TUBBY (P15Y) Col0 background N2110793 (HygroR + BastaR)
– MAP-mCH-dOCRL x mCit-P4M (PI4P marker) Col0 background N2110794 (HygroR + BastaR)
– MAP-mCH-dOCRLdead x mCit-P4M (PI4P marker) Col0 background N2110795 (HygroR + BastaR)
– MAP-3xmCH x mCit-P4M (PI4P marker) Col0 background N2110796 (HygroR + BastaR)

Please cite the following paper when using this material :
Doumane, M., A. Lebecq, L. Colin, A. Fangain, F. D. Stevens, J. Bareille, O. Hamant, Y. Belkhadir, T. Munnik, Y. Jaillais# and M.-C. Caillaud# (2021). "Inducible depletion of PI(4,5)P2 by the synthetic iDePP system in Arabidopsis." Nature Plants 7 587-597 (2021)

The sequences of the vectors used to construct the PI4P depletion system (MAP-SAC1, Platre et al., 2018 Dev Cell) and PI(4,5)P2, iDePP, Doumane et al., 2021 Nature Plants) can be downloaded here :