RT-PCR Using Kit from Applied Biosystems

Kit name : High Capacity RNA to cDNA Master Mix 

(Applied Biosystems,  ref : 4390778, 200 reactions)

IMPORTANT :

Work on ice

Work with gloves

Work in RNA dedicated area

Use RNAse-free pipets and filter tips.

RT PCR MIX:

RNA :                                     16uL                       

Complete Master Mix :               4uL

CYCLING PROGRAM : (on PCR machine)

25°C for 5 minutes

42°C for 30 minutes

85°C for 5 minutes

hold at 4°C