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SD+Dropout

(For 1L)

Difco Yeast Nitrogen Base w/o Amino Acids           6,7g

Glucose                                                               20g

Bacto Agar (if plates)                                           20g

Dropout (aa mix)                                                   2g

Distilled water                                                  qsp 1L

Add NaOH 1N to have pH=5,8 (about 1mL)

Autoclave at 0,5bar

S+Dropout

(without sugar) (For 500L)

Difco Yeast Nitrogen Base w/o Amino Acids            3,35g

Bacto Agar (if plates)                                              10g

Dropout (aa mix)                                                     1g

Distilled water                                              qsp 450mL

Add NaOH 1N to have pH=5,8 (about 500mL)

Autoclave at 0,5bar

YPD

(for 1L)

Bacto Peptone Difco                                                  10g

Bacto Yeast Extract Difco                                           10g

Glucose                                                                    20g

Bacto Agar (if plates)                                                 20g

Distilled water                                                           qsp 1L

Autoclave at 0,5bar

YPD+G418

(for 1L)

Same recipe as above.

After autoclavage let cool down until 55°C

Add G418 to have a final concentration of 200mg/L

(1 mL of 200mg/mL G418 in 1L of YPD)

YPD+NAT

(for 1L)

Same recipe as above.

After autoclavage let cool down until 55°C

Add NAT to have a final concentration of 60mg/L

(300uL of 200mg/mL NAT in 1L of YPD)

YP Gal/Raf

(for 1L

Bacto Peptone Difco                                                     10g

Bacto Yeast Extract Difco                                              10g

Galactose                                                                    20g

Raffinose                                                                     10g

Bacto Agar (if plates)                                                    20g

Distilled water                                                            qsp 1L

Autoclave at 0,5bar

Kac (for Sporulation)

(for 1L)

Potassium acetate                                                            20g

Bacto Agar                                                                       20g

Distilled water                                                            qsp 1L

Adjust to have pH=7

Autoclave at 0,5bar

5 FoA

(For 500mL)

Difco Yeast Nitrogen Base w/o Amino Acids           3.35g

Glucose                                                               10g

Bacto Agar                                                           10g

Dropout mix+ALL                                                   1g

Distilled water                                                  qsp 500mL

 

Autoclave at 0,5bar.

Dissolve 500mg of 5FoA.

 

DROPOUT

Add all the amino acids except 1 or more according to the expected mixture .

Adénine (Ad)  1 g

Uracile (U)  2 g

Alanine (A)  2 g

Arginine (R)  2 g

Aspartate (D)  2 g

Asparagine (N)  2 g

Cystéine (C)  2 g

Glutamate (E)  2 g

Glutamine (Q)  2 g

Glycine (G)  2 g

Histidine (H)  2 g

Isoleucine (I)  2 g

Leucine (L)  4 g

Lysine (K)  2 g

Méthionine (M)  2 g

Phénylalanine (F)  2 g

Proline (P)  2 g

Sérine (S)  2 g

Thréonine (T)  2 g

Tryptophane (W)  2 g

Tyrosine (Y)  2 g

Valine (V)  2 g

DRUGS

Kanamycin (G418 Sulfate)

P25-011, PAA

Stock solution : 200mg/mL ; Final concentration : 200mg/L (1mL in 1L)

NAT (Nourseothricin-sulfate or clonNAT)

5-1000, Gisela Werner

Stock solution : 200mg/mL ; Final concentration : 60mg/L (300uL in 1L)

Hygromycin B

843 555 1g (20mL) in PBS, Roche

Stock solution : 50mg/mL ; Final concentration : 200mg/L (4mL in 1L)

Doxycycline

D-9891, Sigma

Stock solution : 5mg/mL ; Final concentration : 10mg/L (2mL in 1L)

Dernière mise à jour : ( 11-03-2010 )

                

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