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Accueil du site > Animations Scientifiques > Séminaires 2013 > Virgile Adam - Phototransformable fluorescent proteins : new tools for cell biology
Virgile Adam - Phototransformable fluorescent proteins : new tools for cell biology
Speaker : Virgile Adam, Université Grenoble Alpes, Institut de Biologie Structurale (IBS), CNRS, CEA, DSV, 38027 Grenoble, France
E-mail : virgile.adam@ibs.fr
When : thursday 14th november at 11 am
Where : Amphi I ENS de Lyon
Title : Phototransformable fluorescent proteins : new tools for cell biology
Phototransformable fluorescent proteins (PTFPs) are powerful markers in cell imaging. They are homologous to the GFP (Green Fluorescent Protein) but their photochromic properties and/or of photoconversion offer new prospects to track molecules of interest in living cells, to develop super-resolution optical imaging or to create biophotonic systems. Apart from the photoactivation to a fluorescent form, one distinguishes two types of phototransformations : the reversible photocommutation between a fluorescent and a non-fluorescent form and the irreversible photoconversion from a color to another. We combined X-ray crystallography, modeling and in-crystallo spectroscopy in order to characterize the phototransformation mechanisms of IrisFP, first fluorescent protein capable of combining these two types of phototransformations. This protein and variants we have engineered open the door to new types of multicolored imaging with a sub-diffractionnal spatial resolution. Moreover, we have identified structural modifications related to photobleaching, a general problem of fluorescent proteins. Beyond the specific conclusions of these results, this work shows the importance of combining techniques to collect the maximum of mechanistic information about how biomolecules work.
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