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Polymers for Fluorescence Imaging in Infectiology. Project leader : Marie-Thérèse Charreyre.


Our project in Joliot-Curie Laboratory is dedicated to fluorescence imaging, more precisely to the design of original bio-imaging probes combining multifunctional polymers and chromophores, and to their application to reply to three specific biological questions :

  • the assembly and spreading of viral particles from the host cell,
  • the multi-labelling of various phagocyte sub-populations,
  • the study of new apoptosis pathways

The specific contribution of multifunctional polymers to the bio-imaging probes is based on immobilization of recognition moieties, labelling moieties and possibly therapeutic or cytotoxic moieties along the chain. The project objective appears relevant for many applications including ex vivo imaging of cells and in vivo diagnostic possibly combined with a local therapy (theranostic). It is based on a multidisciplinary approach that associates chemistry (molecular and macromolecular), spectrophysics and biology (virology and immuno-apoptosis).

This project is strongly based on our expertise in macromolecular chemistry applied to biology that includes several approaches :

  • kinetics and mechanistic studies of so-called “living” polymerization techniques (especially the RAFT process) providing very well-defined polymer chains.
  • synthesis of several kinds of macromolecular architectures, such as random copolymers, block copolymers, graft copolymers, end-functional copolymers (including reactive copolymers, glycopolymers and fluorescent polymers).
  • elaboration of polymer/biomolecule and polymer/chromophore conjugates via immobilization of biomolecules and chromophores by covalent bonds onto the various polymer architectures.

Team Leader ( Chef d’Equipe ) :

  • Marie-Thérèse CHARREYRE - Research Director, DR CNRS

Team Members ( Membres de l’Equipe ) :

  • Salim Adjili - PhD student
  • Cristina Cepraga - PhD student
  • Arnaud Favier - Research Associate Second Class
  • Sophie Marotte - Engineer in biology

Legend :

Synthesis of biotin end-functionalized glycopolymer by RAFT copolymerization.

Sélection de publications :

  • Maël Bathfield, Delphine Daviot, Franck D’Agosto, Roger Spitz, Catherine Ladavière, Marie-Thérèse Charreyre, Thierry Delair, Synthesis of Lipid-alpha-end-Functionalized Chains by RAFT polymerization. Stabilization of Lipid/Polymer Particle Assemblies, Macromolecules, 41, 8346-8353 (2008).
  • Arnaud Favier, Marie-Thérèse Charreyre, Bertrand de Lambert Toward New Materials Prepared via the RAFT Process : From Drug Delivery to Optoelectronics ? In Handbook of RAFT Polymerization, C ; Barner-Kowollik Ed., Wiley-VCH (2008).
  • Xiaoze Jiang, Abdelghani Housni, Guillaume Gody, Paul Boullanger, Marie-Thérèse Charreyre, Thierry Delair, Ravin Narain, Synthesis of Biotinylated α-D-Mannoside or N-Acetyl β-D-Glucosaminoside Decorated Gold Nanoparticles : Study of Their Biomolecular Recognition with Con A and WGA Lectins, Bioconjugate Chem. 21, 521–530 (2010).