Annuaire
Haitham Shaban, EPFL - Lausanne
| Quand ? |
Le 04/03/2022, de 11:00 à 12:00 |
|---|---|
| S'adresser à | Salle des Thèses, Equipe Jost |
| Ajouter un événement au calendrier |
vCal
iCal
|
Contact : Geneviève Forel
Haitham Shaban, PhD
Institute of Bioengineering, Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland
Email: haitham.shaban@epfl.ch
The spatio-temporal organization of chromatin in the eukaryotic cell nucleus is of vital importance for transcription, DNA replication and genome maintenance. Each of these activities is tightly regulated in both time and space. The eukaryotic genome is hierarchically structured yet highly dynamic. Regulating transcription in this environment demands a high level of coordination to permit many proteins to interact with chromatin fiber at appropriate sites in a timely manner. In this seminar, I will introduce our recent quantitative imaging techniques for the visualization of chromatin, transcription factors, and RNA polymerase II dynamics at genome-scale with nanometer resolution in living cells. Also, I will discuss how advances of these techniques overcome caveats of sequencing-based methods (Hi-C and related) by enabling direct visualization of chromatin in space and time at high resolution. I will also discuss the contribution of our results in deciphering the principles underlying this coordination within the crowded nuclear space in living cells.
References
1. H. A. Shaban, R. Barth, K. Bystricky, Formation of correlated chromatin domains at nanoscale dynamic resolution during transcription. Nucleic Acids Res. 46 (2018), doi:10.1093/nar/gky269.
2. H. A. Shaban, R. Barth, L. Recoules, K. Bystricky, Hi-D: nanoscale mapping of nuclear dynamics in single living cells. Genome Biol. 21, 95 (2020).
3. R. Barth, K. Bystricky, H. A. Shaban, Coupling chromatin structure and dynamics by live super-resolution imaging. Sci. Adv. 6 (2020), doi:10.1126/sciadv.aaz2196.
4. H. A. Shaban, R. Barth, K. Bystricky, Navigating the crowd: visualizing coordination between genome dynamics, structure, and transcription. Genome Biol. (2020), doi:10.1186/s13059-020-02185-y.
5. R. Barth, G. Fourel, H. A. Shaban, Dynamics as a cause for the nanoscale organization of the genome. Nucleus. 11, 83–98 (2020).
6. R. Barth, H. A. Shaban, Spatially coherent diffusion of human RNA Pol II depends on transcriptional state rather than chromatin motion. bioRxiv (2022), doi:10.1101/2022.01.19.476954.
The spatio-temporal organization of chromatin in the eukaryotic cell nucleus is of vital importance for transcription, DNA replication and genome maintenance. Each of these activities is tightly regulated in both time and space. The eukaryotic genome is hierarchically structured yet highly dynamic. Regulating transcription in this environment demands a high level of coordination to permit many proteins to interact with chromatin fiber at appropriate sites in a timely manner. In this seminar, I will introduce our recent quantitative imaging techniques for the visualization of chromatin, transcription factors, and RNA polymerase II dynamics at genome-scale with nanometer resolution in living cells. Also, I will discuss how advances of these techniques overcome caveats of sequencing-based methods (Hi-C and related) by enabling direct visualization of chromatin in space and time at high resolution. I will also discuss the contribution of our results in deciphering the principles underlying this coordination within the crowded nuclear space in living cells.
References
1. H. A. Shaban, R. Barth, K. Bystricky, Formation of correlated chromatin domains at nanoscale dynamic resolution during transcription. Nucleic Acids Res. 46 (2018), doi:10.1093/nar/gky269.
2. H. A. Shaban, R. Barth, L. Recoules, K. Bystricky, Hi-D: nanoscale mapping of nuclear dynamics in single living cells. Genome Biol. 21, 95 (2020).
3. R. Barth, K. Bystricky, H. A. Shaban, Coupling chromatin structure and dynamics by live super-resolution imaging. Sci. Adv. 6 (2020), doi:10.1126/sciadv.aaz2196.
4. H. A. Shaban, R. Barth, K. Bystricky, Navigating the crowd: visualizing coordination between genome dynamics, structure, and transcription. Genome Biol. (2020), doi:10.1186/s13059-020-02185-y.
5. R. Barth, G. Fourel, H. A. Shaban, Dynamics as a cause for the nanoscale organization of the genome. Nucleus. 11, 83–98 (2020).
6. R. Barth, H. A. Shaban, Spatially coherent diffusion of human RNA Pol II depends on transcriptional state rather than chromatin motion. bioRxiv (2022), doi:10.1101/2022.01.19.476954.