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Vous êtes ici : Accueil / Équipes / Epigenetic regulation of cell identity and environmental stress responses - F. Palladino / Publications / Novel roles of Caenorhabditis elegans heterochromatin protein HP1 and linker histone in the regulation of innate immune gene expression.

Novel roles of Caenorhabditis elegans heterochromatin protein HP1 and linker histone in the regulation of innate immune gene expression.

Maja Studencka, Anne Konzer, Gael Moneron, Dirk Wenzel, Lennart Opitz, Gabriela Salinas-Riester, Cecile Bedet, Marcus Kruger, Stefan W Hell, Jacek R Wisniewski, Henning Schmidt, Francesca Palladino, Ekkehard Schulze, and Monika Jedrusik-Bode (2012)

Mol Cell Biol, 32(2):251-65.

Linker histone (H1) and heterochromatin protein 1 (HP1) are essential componentsof heterochromatin which contribute to the transcriptional repression of genes. It has been shown that the methylation mark of vertebrate histone H1 is specifically recognized by the chromodomain of HP1. However, the exact biological role of linker histone binding to HP1 has not been determined. Here, we investigate the function of the Caenorhabditis elegans H1 variant HIS-24 and theHP1-like proteins HPL-1 and HPL-2 in the cooperative transcriptional regulation of immune-relevant genes. We provide the first evidence that HPL-1 interacts with HIS-24 monomethylated at lysine 14 (HIS-24K14me1) and associates in vivo with promoters of genes involved in antimicrobial response. We also report an increase in overall cellular levels and alterations in the distribution of HIS-24K14me1 after infection with pathogenic bacteria. HIS-24K14me1 localization changes frombeing mostly nuclear to both nuclear and cytoplasmic in the intestinal cells of infected animals. Our results highlight an antimicrobial role of HIS-24K14me1 and suggest a functional link between epigenetic regulation by an HP1/H1 complex andthe innate immune system in C. elegans.

 
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