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You are here: Home / Teams / Chromatin Dynamics in Chromosome Architecture - P. Bernard / Publications / Hsk1-Dfp1 is required for heterochromatin-mediated cohesion at centromeres.

Hsk1-Dfp1 is required for heterochromatin-mediated cohesion at centromeres.

Julie M Bailis, Pascal Bernard, Richard Antonelli, Robin C Allshire, and Susan L Forsburg (2003)

Nat Cell Biol, 5(12):1111-6.

Heterochromatin performs a central role in chromosome segregation and stability by promoting cohesion at centromeres. Establishment of both heterochromatin-mediated silencing and cohesion requires passage through S phase, although the mechanism is unknown. Here we demonstrate that Schizosaccharomyces pombe Hsk1 (CDC7), a conserved Dbf4-dependent protein kinase (DDK) that regulates replication initiation, interacts with and phosphorylates the heterochromatin protein 1 (HP1) equivalent Swi6 (ref. 6). Hsk1 and its regulatory subunit Dfp1 function downstream of Swi6 localization to promote heterochromatin function andcohesion specifically at centromeres. This role for Hsk1-Dfp1 is separable from its replication initiation activity, providing a temporal link between S phase and centromere cohesion that is mediated by heterochromatin.

 
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