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Key technologies

Know-how and applied methods:

Plasmid creation/optimization

To ensure optimal expression of target genes in mammalian and yeast cells our team relies on tailored plasmids generated using the combination of classical/combinatorial PCR reactions with homologous recombination in yeasts or in vitro.

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Liquid-Phase Yeast Two Hybrid assay

Our optimized protocol for liquid-phase Y2H in 96-well plates permits rapid screening of protein-protein interactions.

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Single-cell phenotyping

We perform single-cell studies with a variety of different technologies:

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Biomolecular computer simulations

To gains insight into the structure and (thermo)dynamics of proteins and their interactions we apply the following technologies:

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