Annuaire
Plasmid creation/optimization
To ensure optimal expression of target genes in mammalian and yeast cells our team relies on tailored plasmids generated using the combination of classical/combinatorial PCR reactions with homologous recombination in yeasts or in vitro.
The High Fidelity combinatorial PCR permits to quickly assemble different DNA sequences in order to generate a brand new expression cassette, or replace a specific domain of a protein by a random mutagenesis library.
The homologous recombination in yeast or in vitro, allows also to insert at the same time several DNA fragments within a plasmid of interest. The insertion is orientated even within a single restriction site.
The combination of these two technologies allows us to easily generate new generations of Yeast Two Hybrid or mammalian expression vectors with a high efficiency and low costs.