Key technologies
Plasmid creation/optimization
To ensure optimal expression of target genes in mammalian and yeast cells our team relies on tailored plasmids generated using the combination of classical/combinatorial PCR reactions with homologous recombination in yeasts or in vitro.
Liquid-Phase Yeast Two Hybrid assay
Our optimized protocol for liquid-phase Y2H in 96-well plates permits rapid screening of protein-protein interactions.
Biomolecular computer simulations
To gains insight into the structure and (thermo)dynamics of proteins and their interactions we apply the following technologies: