RESOURCES :
materials:
PIPline marker set
The PIPline is a multi-color and multi-affinity marker set that highlights phosphatidylinositol phosphate (PIP) associated with membranes in Arabidopsis thaliana.
ABRC stock set #CS2105604
NASC stock set # N21056604
All the sequence of the PIPline entry vectors in VNTI format > PIPline_entry_vectors.ma4
All the sequence of the PIPline destination vectors in VNTI format > PIPline_destination_vectors.ma4
All the sequence of the PIPline entry vectors in genebank format > PIPline_entry_vectors.zip
All the sequence of the PIPline destination vectors in genebank format > PIPline_destination_vectors.zip
Simon M*, Platre M*, Assil S, van Wijk R, Chen WC, Chory J , Dreux M, Munnik T and Jaillais Y. 2014. A multi-colour/multi-affinity marker set to visualize phosphoinositide dynamics in Arabidopsis. Plant Journal, Jan;77(2):322-37. doi: 10.1111/tpj.12358. PMID: 24147788
Cell Signaling lab
RDP - ENS Lyon - 46 Allee d’italie
69364 Lyon cedex 07 - France
Web Site : Yvon Jaillais
- Plant Biology - Cell Biology - Plant development - Cell Signaling - Signal transduction - Receptor Kinase - Plant adaptation - Endocytosis - Self Incompatibility - Plant Reproduction - Brassinosteroid - Plant hormones - hormone crosstalk - Intracellular trafficking - Live confocal imaging - BRI1- BKI1- SRK - SCR - Retromer - SNX - Sorting Nexin - Vacuolar Protein Sorting - VPS - RDP, ENS Lyon, Ecole Normale Superieure - Laboratoire de Reproduction et development des plantes - France -
PIPline #1 (P1Y)
ABRC# CS2105605 - NASC# N2105605
Target: PI(3)P
Domain: 1xFYVE(HRS)
Transgene resistance: Basta
Construct name: UBQ10prom::CITRINE-1xFYVE(HRS)/pB7m34GW
Sequence destination vector: P1Y.gb
Sequence entry vectors: UBQ10prom-pDONRP4-P1R.gb
PIPline #3 (P3Y)
ABRC# CS2105606 - NASC# N2105606
Target: PI(3)P
Domain: 1xPX(p40)
Transgene resistance: Basta
Construct name: UBQ10prom::1xPX(p40)-CITRINE/pB7m34GW
Sequence: P3Y.gb
Sequence entry vectors: UBQ10prom-pDONRP4-P1R.gb
PIPline #5 (P5Y)
ABRC# CS2105607 - NASC# N2105607
Target: PI(4)P
Domain: 1xPH(FAPP1)
Transgene resistance: Basta
Construct name: UBQ10prom::CITRINE-1xPH(FAPP1)/pB7m34GW
Sequence: P5Y.gb
Sequence entry vectors: UBQ10prom-pDONRP4-P1R.gb
PIPline #7 (P7Y)
ABRC# CS2105608 - NASC# N2105608
Target: PI(4)P
Domain: 1xPH(OSBP)
Transgene resistance: Basta
Construct name: UBQ10prom::CITRINE-1xPH(FAPP1)/pB7m34GW
Sequence: P7Y.gb
Sequence entry vectors: UBQ10prom-pDONRP4-P1R.gb
PIPline #14 (P14Y)
ABRC# CS2105609 - NASC# N2105609
Target: PI(4,5)P2
Domain: 1xPH(PLC)
Transgene resistance: Basta
Construct name: UBQ10prom::CITRINE-1xPH(PLC)/pB7m34GW
Sequence: P14Y.gb
Sequence entry vectors: UBQ10prom-pDONRP4-P1R.gb
PIPline #15 (P15Y)
ABRC# CS2105610 - NASC# N2105610
Target: PI(4,5)P2
Domain: 1xTUBBY-C(TUBBY)
Transgene resistance: Basta
Construct name: UBQ10prom::CITRINE-1xTUBBY-C(TUBBY)/pB7m34GW
Sequence: P15Y.gb
Sequence entry vectors: UBQ10prom-pDONRP4-P1R.gb
PIPline #18 (P18Y)
ABRC# CS2105611 - NASC# N2105611
Target: PI(3)P
Domain: 2xFYVE(HRS)
Transgene resistance: Basta
Construct name: UBQ10prom::CITRINE-2xFYVE(HRS)/pB7m34GW
Sequence destination vector: P18Y.gb
Sequence entry vectors: UBQ10prom-pDONRP4-P1R.gb
PIPline #21 (P21Y)
ABRC# CS2105612 - NASC# N2105612
Target: PI(4)P
Domain: 2xPH(FAPP1)
Transgene resistance: Basta
Construct name: UBQ10prom::CITRINE-2xPH(FAPP1)/pB7m34GW
Sequence: P21Y.gb
Sequence entry vectors: UBQ10prom-pDONRP4-P1R.gb
PIPline #24 (P24Y)
ABRC# CS2105613 - NASC# N2105613
Target: PI(4,5)P2
Domain: 2xPH(PLC)
Transgene resistance: Basta
Construct name: UBQ10prom::CITRINE-2xPH(PLC)/pB7m34GW
Sequence: P24Y.gb
Sequence entry vectors: UBQ10prom-pDONRP4-P1R.gb
PIPline #1 (P1R)
ABRC# CS2105614 - NASC# N2105614
Target: PI(3)P
Domain: 1xFYVE(HRS)
Transgene resistance: Hygromycin
Construct name: UBQ10prom::2xCHERRY-1xFYVE(HRS)/pH7m34GW
Sequence destination vector: P1R.gb
Sequence entry vectors: UBQ10prom-pDONRP4-P1R.gb
PIPline #3 (P3R)
ABRC# CS2105615 - NASC# N2105615
Target: PI(3)P
Domain: 1xPX(p40)
Transgene resistance: Hygromycin
Construct name: UBQ10prom::1xPX(p40)-2xCHERRY/pH7m34GW
Sequence: P3R.gb
Sequence entry vectors: UBQ10prom-pDONRP4-P1R.gb
PIPline #5 (P5R)
ABRC# CS2105616 - NASC# N2105616
Target: PI(4)P
Domain: 1xPH(FAPP1)
Transgene resistance: Hygromycin
Construct name: UBQ10prom::2xCHERRY-1xPH(FAPP1)/pH7m34GW
Sequence: P5R.gb
Sequence entry vectors: UBQ10prom-pDONRP4-P1R.gb
PIPline #7 (P7R)
ABRC# CS2105617 - NASC# N2105617
Target: PI(4)P
Domain: 1xPH(OSBP)
Transgene resistance: Hygromycin
Construct name: UBQ10prom::2xCHERRY-1xPH(FAPP1)/pH7m34GW
Sequence: P5R.gb
Sequence entry vectors: UBQ10prom-pDONRP4-P1R.gb
PIPline #14 (P14R)
ABRC# CS2105618 - NASC# N2105618
Target: PI(4,5)P2
Domain: 1xPH(PLC)
Transgene resistance: Hygromycin
Construct name: UBQ10prom::2xCHERRY-1xPH(PLC)/pH7m34GW
Sequence: P14R.gb
Sequence entry vectors: UBQ10prom-pDONRP4-P1R.gb
PIPline #15 (P15R)
ABRC# CS2105619 - NASC# N2105619
Target: PI(4,5)P2
Domain: 1xTUBBY-C(TUBBY)
Transgene resistance: Hygromycin
Construct name: UBQ10prom::2xCHERRY-1xTUBBY-C(TUBBY)/pH7m34GW
Sequence: P15R.gb
Sequence entry vectors: UBQ10prom-pDONRP4-P1R.gb
PIPline #18 (P18R)
ABRC# CS2105620 - NASC# N2105620
Target: PI(3)P
Domain: 2xFYVE(HRS)
Transgene resistance: Hygromycin
Construct name: UBQ10prom::2xCHERRTY-2xFYVE(HRS)/pH7m34GW
Sequence destination vector: P18R.gb
Sequence entry vectors: UBQ10prom-pDONRP4-P1R.gb
PIPline #21 (P21R)
ABRC# CS2105621 - NASC# N2105621
Target: PI(4)P
Domain: 2xPH(FAPP1)
Transgene resistance: Hygromycin
Construct name: UBQ10prom::2xCHERRY-2xPH(FAPP1)/pH7m34GW
Sequence: P21R.gb
Sequence entry vectors: UBQ10prom-pDONRP4-P1R.gb
PIPline #24 (P24R)
ABRC# CS2105622 - NASC# N2105622
Target: PI(4,5)P2
Domain: 2xPH(PLC)
Transgene resistance: Hygromycin
Construct name: UBQ10prom::2xCHERRY-2xPH(PLC)/pB7m34GW
Sequence: P24R.gb
Sequence entry vectors: UBQ10prom-pDONRP4-P1R.gb
PIPline #3 (P3C)
ABRC# CS2105623 - NASC# N2105623
Target: PI(3)P
Domain: 1xPX(p40)
Transgene resistance: Kanamycin
Construct name: UBQ10prom::1xPX(p40)-2xCyPet/pK7m34GW
Sequence: P3C.gb
Sequence entry vectors: UBQ10prom-pDONRP4-P1R.gb
PIPline #5 (P5C)
ABRC# CS2105624 - NASC# N2105624
Target: PI(4)P
Domain: 1xPH(FAPP1)
Transgene resistance: Kanamycin
Construct name: UBQ10prom::2xCyPet-1xPH(FAPP1)/pK7m34GW
Sequence: P5C.gb
Sequence entry vectors: UBQ10prom-pDONRP4-P1R.gb
PIPline #14 (P14C)
ABRC# CS2105625 - NASC# N2105625
Target: PI(4,5)P2
Domain: 1xPH(PLC)
Transgene resistance: Kanamycin
Construct name: UBQ10prom::2xCyPet-1xPH(PLC)/pK7m34GW
Sequence: P14C.gb
Sequence entry vectors: UBQ10prom-pDONRP4-P1R.gb
PIPline #18 (P18C)
ABRC# CS2105626 - NASC# N2105626
Target: PI(3)P
Domain: 2xFYVE(HRS)
Transgene resistance: Kanamycin
Construct name: UBQ10prom::2xCyPet-2xFYVE(HRS)/pK7m34GW
Sequence destination vector: P18C.gb
Sequence entry vectors: UBQ10prom-pDONRP4-P1R.gb
